Restriction map for
pFN21AB6563
(Circular) (Six-base) MAPSORT of: pFN21AB6563.seq Check: 4801 from: 1 to: 5827
pFN21AB6563 5827 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2478 2478
Size: 5827
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2631 3637 278
Size: 53 83 186 936 1095 1006 2468
Fragments arranged by size:
2468 1095 1006 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2250 3170 1937
Size: 313 920 4594
Fragments arranged by size:
4594 920 313
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 3007 3890 5274 5542 1632
Size: 521 854 883 1384 268 1917
Fragments arranged by size:
1917 1384 883 854 521 268
AfeI AGC'GCT
Cuts at: 2393 2393
Size: 5827
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 2946 3035 4726 5703 1203
Size: 1743 89 1691 977 1327
Fragments arranged by size:
1743 1691 1327 977 89
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 4432
AlwNI CAG_nnn'CTG
Cuts at: 2416 2728 3570 5142 2416
Size: 312 842 1572 3101
Fragments arranged by size:
3101 1572 842 312
ApaI G_GGCC'C
Cuts at: 2153 2906 2153
Size: 753 5074
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 2462 3136 3362 3514 5623 1493
Size: 969 674 226 152 2109 1697
Fragments arranged by size:
2109 1697 969 674 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2324 2984 3152 1870
Size: 73 127 254 660 168 4545
Fragments arranged by size:
4545 660 254 168 127 73
BamHI G'GATC_C
Cuts at: 2094 2661 3157 3524 2094
Size: 567 496 367 4397
Fragments arranged by size:
4397 567 496 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2204 3148 4603 618
Size: 325 217 627 253 164 944 1455 1842
Fragments arranged by size:
1842 1455 944 627 325 253 217 164
BanII G_rGCy'C
Cuts at: 1391 2153 2906 3146 1391
Size: 762 753 240 4072
Fragments arranged by size:
4072 762 753 240
BbeI G_GCGC'C
Cuts at: 2208 2208
Size: 5827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3061 928
Size: 971 1162 3694
Fragments arranged by size:
3694 1162 971
BbvCI CC'TCA_GC
Cuts at: 2919 2919
Size: 5827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2988 4438 136
Size: 107 122 71 631 605 1316 1450 1525
Fragments arranged by size:
1525 1450 1316 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2153 2906 3888 5044 1790
Size: 363 753 982 1156 2573
Fragments arranged by size:
2573 1156 982 753 363
BmgBI CAC'GTC
Cuts at: 1318 2548 3079 1318
Size: 1230 531 4066
Fragments arranged by size:
4066 1230 531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2886 3558 4516
Size: 416 53 83 399 24 1469 672 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1469 958 672 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2346 1056
Size: 1290 4537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 2750 2870 4487 1239
Size: 1286 225 120 1617 2579
Fragments arranged by size:
2579 1617 1286 225 120
Bpu10I CC'TnA_GC
Cuts at: 2541 2907 2919 2541
Size: 366 12 5449
Fragments arranged by size:
5449 366 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 2192 5821 2192
Size: 3629 2198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 2628 3016 3634
Size: 53 83 186 936 672 423 388 618
Cuts at: 3634 4016 4651 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 672 635 618 423 388 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4260 4658 4932 5079 5490 4260
Size: 398 274 147 411 4597
Fragments arranged by size:
4597 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 4396
Fragments arranged by size:
4396 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2237 2534 2747 2867 1186
Size: 1048 3 297 213 120 4146
Fragments arranged by size:
4146 1048 297 213 120 3
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2621 1163
Size: 573 885 4369
Fragments arranged by size:
4369 885 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2174 2553 3096 3888 3973 5044 2174
Size: 379 543 792 85 1071 2957
Fragments arranged by size:
2957 1071 792 543 379 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2174 2553 2906 3096 3146 3888
Size: 399 363 21 379 353 190 50 742
Cuts at: 3888 3973 5044 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 363 353 190
85 50 21
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 2352 3716 5446 2352
Size: 1364 1730 2733
Fragments arranged by size:
2733 1730 1364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 2622 3129 3182 844
Size: 1634 144 507 53 3489
Fragments arranged by size:
3489 1634 507 144 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2163 2299 3714 1394
Size: 429 190 150 136 1415 3507
Fragments arranged by size:
3507 1415 429 190 150 136
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 2429 4471 4658 1700
Size: 110 397 222 2042 187 2869
Fragments arranged by size:
2869 2042 397 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 2949 2961 96
Size: 2853 12 2962
Fragments arranged by size:
2962 2853 12
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 2953 3887 4899 2953
Size: 934 1012 3881
Fragments arranged by size:
3881 1012 934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstYI r'GATC_y
Cuts at: 1972 2094 2661 3157 3524 3909 3926 5378 5464
Size: 122 567 496 367 385 17 1452 86
Cuts at: 5464 5476 5822 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 567 496 385 367 346 122
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2122 2200 2292 513
Size: 552 939 118 78 92 4048
Fragments arranged by size:
4048 939 552 118 92 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2417 3343 4137 4157 2417
Size: 926 794 20 4087
Fragments arranged by size:
4087 926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DraIII CAC_nnn'GTG
Cuts at: 2711 2970 2711
Size: 259 5568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 3274 4163
Size: 54 1425 50 275 18 573 871 889
Cuts at: 4163 5792 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 871 573 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 3026 3757 1353
Size: 809 864 731 3423
Fragments arranged by size:
3423 864 809 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2525 2750 2870 3007 3890 4487
Size: 393 521 372 225 120 137 883 597
Cuts at: 4487 5274 5542 1239
Size: 787 268 1524
Fragments arranged by size:
1524 883 787 597 521 393 372 268
225 137 120
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 5827
EcoO109I rG'GnC_Cy
Cuts at: 2362 2902 2903 2362
Size: 540 1 5286
Fragments arranged by size:
5286 540 1
EcoRI G'AATT_C
Cuts at: 2462 3136 3514 2462
Size: 674 378 4775
Fragments arranged by size:
4775 674 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 5827
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2029 2208 2395 4974 1678
Size: 351 179 187 2579 2531
Fragments arranged by size:
2579 2531 351 187 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373
Size: 32 135 32 2896 32 42 32 2626
Fragments arranged by size:
2896 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2204 2204
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2957 3785 4144 4303 518
Size: 648 1098 693 828 359 159 2042
Fragments arranged by size:
2042 1098 828 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2124 3243 3920 5068 5313 1606
Size: 518 1119 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 518 245
NaeI GCC'GGC
Cuts at: 2209 2431 2209
Size: 222 5605
NarI GG'CG_CC
Cuts at: 2205 2205
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2004 2292 513
Size: 552 939 288 4048
Fragments arranged by size:
4048 939 552 288
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2207 2429 2207
Size: 222 5605
NheI G'CTAG_C
Cuts at: 1052 2342 1052
Size: 1290 4537
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 2566 2950 3039 3185 4730 1132
Size: 97 1337 384 89 146 1545 2229
Fragments arranged by size:
2229 1545 1337 384 146 97 89
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 2946 3035 4726 2946
Size: 89 1691 4047
Fragments arranged by size:
4047 1691 89
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2412 2728 1108
Size: 1304 316 4207
Fragments arranged by size:
4207 1304 316
PfoI T'CCnGG_A
Cuts at: 2787 2847 2940 2787
Size: 60 93 5674
Fragments arranged by size:
5674 93 60
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2149 2902 2149
Size: 753 5074
PstI C_TGCA'G
Cuts at: 830 2307 3179 830
Size: 1477 872 3478
Fragments arranged by size:
3478 1477 872
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SacII CC_GC'GG
Cuts at: 2125 2125
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SapI GCTCTTCn'nnn_
Cuts at: 2162 2162
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 2588 4075 5787 2588
Size: 1487 1712 2628
Fragments arranged by size:
2628 1712 1487
SfcI C'TryA_G
Cuts at: 826 1045 2303 3175 4310 4991 5182 826
Size: 219 1258 872 1135 681 191 1471
Fragments arranged by size:
1471 1258 1135 872 681 219 191
SfoI GGC'GCC
Cuts at: 2206 2206
Size: 5827
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 2072 3154 1872
Size: 200 1082 4545
Fragments arranged by size:
4545 1082 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 2566 3185 1132
Size: 1434 619 3774
Fragments arranged by size:
3774 1434 619
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 3266 513
Size: 552 939 288 974 3074
Fragments arranged by size:
3074 974 939 552 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 2586 2949
Size: 274 80 33 51 1443 510 99 363
Cuts at: 2949 2961 4073 5785 96
Size: 12 1112 1712 138
Fragments arranged by size:
1712 1443 1112 510 363 274 138 99
80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3042 3074 5787 5819 3042
Size: 32 2713 32 3050
Fragments arranged by size:
3050 2713 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2872 2872
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 2070 3152 1870
Size: 200 1082 4545
Fragments arranged by size:
4545 1082 200
XmnI GAAnn'nnTTC
Cuts at: 2261 3956 2261
Size: 1695 4132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2629 3635 276
Size: 53 83 186 936 1095 1006 2468
Fragments arranged by size:
2468 1095 1006 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SanDI SexAI SfiI SrfI SwaI