Restriction map for
pFN21AB6561
(Circular) (Six-base) MAPSORT of: pFN21AB6561.seq Check: 7069 from: 1 to: 5827
pFN21AB6561 5827 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3890 5274 5542 1632
Size: 2258 1384 268 1917
Fragments arranged by size:
2258 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2813 820
Size: 197 1796 3834
Fragments arranged by size:
3834 1796 197
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2726 1184
Size: 1542 4285
AlwNI CAG_nnn'CTG
Cuts at: 2313 3570 5142 2313
Size: 1257 1572 2998
Fragments arranged by size:
2998 1572 1257
ApaLI G'TGCA_C
Cuts at: 3098 3884 5040 3098
Size: 786 1156 3885
Fragments arranged by size:
3885 1156 786
ApoI r'AATT_y
Cuts at: 1493 2452 3136 3362 3514 5623 1493
Size: 959 684 226 152 2109 1697
Fragments arranged by size:
2109 1697 959 684 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 3152 1870
Size: 73 1209 4545
Fragments arranged by size:
4545 1209 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2256 2495 3148 4603 618
Size: 325 217 627 469 239 653 1455 1842
Fragments arranged by size:
1842 1455 653 627 469 325 239 217
BanII G_rGCy'C
Cuts at: 1391 3114 3146 1391
Size: 1723 32 4072
Fragments arranged by size:
4072 1723 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 2769 3039 928
Size: 971 436 434 270 3716
Fragments arranged by size:
3716 971 436 434 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 2073 1548
Size: 525 5302
BfrBI ATG'CAT
Cuts at: 2591 2591
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 3102 3888 5044 1790
Size: 1312 786 1156 2573
Fragments arranged by size:
2573 1312 1156 786
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4516 442
Size: 416 53 83 399 24 2141 958 1753
Fragments arranged by size:
2141 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 2562 2633 2837 4487 1239
Size: 1078 245 71 204 1650 2579
Fragments arranged by size:
2579 1650 1078 245 204 71
Bpu10I CC'TnA_GC
Cuts at: 2509 2633 2509
Size: 124 5703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2389 3952 4817 5115 2389
Size: 1563 865 298 3101
Fragments arranged by size:
3101 1563 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 2335 4490 882
Size: 617 774 62 2155 2219
Fragments arranged by size:
2219 2155 774 617 62
BsaAI yAC'GTr
Cuts at: 493 1204 2945 3618 493
Size: 711 1741 673 2702
Fragments arranged by size:
2702 1741 711 673
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3634 4016 4651 275
Size: 53 83 186 936 2101 382 635 1451
Fragments arranged by size:
2101 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3102 4260 4658 4932 5079 5490 3102
Size: 1158 398 274 147 411 3439
Fragments arranged by size:
3439 1158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5602
Fragments arranged by size:
5602 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5827
BseYI C'CCAG_C
Cuts at: 2207 5030 5526 2207
Size: 2823 496 2508
Fragments arranged by size:
2823 2508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 3102 3888 3973 5044 2142
Size: 960 786 85 1071 2925
Fragments arranged by size:
2925 1071 960 786 85
BsmI GAATG_Cn'
Cuts at: 2593 3338 3431 2593
Size: 745 93 4989
Fragments arranged by size:
4989 745 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 3102 3114 3146 3888 3973 5044
Size: 399 352 960 12 32 742 85 1071
Cuts at: 5044 1391
Size: 2174
Fragments arranged by size:
2174 1071 960 742 399 352 85 32
12
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3001 3716 5446 3001
Size: 715 1730 3382
Fragments arranged by size:
3382 1730 715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2317 3182 844
Size: 1473 865 3489
Fragments arranged by size:
3489 1473 865
BsrBI CCG'CTC
Cuts at: 1394 1823 3714 1394
Size: 429 1891 3507
Fragments arranged by size:
3507 1891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2503 4322 4496 65
Size: 1183 1255 1819 174 1396
Fragments arranged by size:
1819 1396 1255 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4471 4658 1700
Size: 110 2661 187 2869
Fragments arranged by size:
2869 2661 187 110
BsrGI T'GTAC_A
Cuts at: 96 2400 96
Size: 2304 3523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 2626 1184
Size: 1442 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 2150 2296 2150
Size: 146 5681
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2090 3123 513
Size: 552 1025 1033 3217
Fragments arranged by size:
3217 1033 1025 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2510 487
Size: 683 56 1284 3804
Fragments arranged by size:
3804 1284 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2904 3343 4137 4157 2076
Size: 828 439 794 20 3746
Fragments arranged by size:
3746 828 794 439 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2224 2259 529
Size: 35 1660 35 4097
Fragments arranged by size:
4097 1660 35 35
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3274 4163 5792
Size: 54 1425 50 275 18 1444 889 1629
Cuts at: 5792 8
Size: 43
Fragments arranged by size:
1629 1444 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2752 3045 3757 1353
Size: 1399 293 712 3423
Fragments arranged by size:
3423 1399 712 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2562 2633 2837 3890 4487 5274
Size: 393 685 245 71 204 1053 597 787
Cuts at: 5274 5542 1239
Size: 268 1524
Fragments arranged by size:
1524 1053 787 685 597 393 268 245
204 71
EcoRI G'AATT_C
Cuts at: 2452 3136 3514 2452
Size: 684 378 4765
Fragments arranged by size:
4765 684 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5827
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 4974 1678
Size: 3296 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373
Size: 32 135 32 2896 32 42 32 2626
Fragments arranged by size:
2896 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 4941 5125 1305
Size: 1506 2130 184 2007
Fragments arranged by size:
2130 2007 1506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4005
Fragments arranged by size:
4005 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3785 4144 4303 518
Size: 648 2619 359 159 2042
Fragments arranged by size:
2619 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2296 3085 3243 3920 5068 5313 1606
Size: 690 789 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 789 690 677 245 158
NcoI C'CATG_G
Cuts at: 513 1065 2090 513
Size: 552 1025 4250
Fragments arranged by size:
4250 1025 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NsiI A_TGCA'T
Cuts at: 2593 2593
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3115 1348
Size: 1767 4060
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2045 1108
Size: 937 4890
PfoI T'CCnGG_A
Cuts at: 2610 2610
Size: 5827
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 3896 3928 5409 5441 2714
Size: 32 1150 32 1481 32 3100
Fragments arranged by size:
3100 1481 1150 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2328 3090 3179 830
Size: 1498 762 89 3478
Fragments arranged by size:
3478 1498 762 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2296 3085 2296
Size: 789 5038
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SapI GCTCTTCn'nnn_
Cuts at: 3045 3045
Size: 5827
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2212 2324 3086 3175 4310 4991 5182
Size: 219 1167 112 762 89 1135 681 191
Cuts at: 5182 826
Size: 1471
Fragments arranged by size:
1471 1167 1135 762 681 219 191 112
89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2368 2813 3931 4832 5094 820
Size: 197 926 425 445 1118 901 262 1553
Fragments arranged by size:
1553 1118 926 901 445 425 262 197
SnaBI TAC'GTA
Cuts at: 493 2945 3618 493
Size: 2452 673 2702
Fragments arranged by size:
2702 2452 673
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 2665 1517
Size: 1148 4679
StyI C'CwwG_G
Cuts at: 513 1065 2090 3266 513
Size: 552 1025 1176 3074
Fragments arranged by size:
3074 1176 1025 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2923 3854 3871 4024 4209 4654 325
Size: 1255 1343 931 17 153 185 445 1498
Fragments arranged by size:
1498 1343 1255 931 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 4073 5785
Size: 274 80 33 51 1443 423 1673 1712
Cuts at: 5785 96
Size: 138
Fragments arranged by size:
1712 1673 1443 423 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2868 2868
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI