Restriction map for
pFN21AB6559
(Circular) (Six-base) MAPSORT of: pFN21AB6559.seq Check: 1901 from: 1 to: 5824
pFN21AB6559 5824 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2651 2651
Size: 5824
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2445 3887 5271 5539 1632
Size: 744 69 1442 1384 268 1917
Fragments arranged by size:
1917 1442 1384 744 268 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2807 4723 5700 1203
Size: 1604 1916 977 1327
Fragments arranged by size:
1916 1604 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 2462 4553 2462
Size: 2091 3733
AleI CACnn'nnGTG
Cuts at: 1166 2810 1166
Size: 1644 4180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2634 1184
Size: 1450 4374
AlwNI CAG_nnn'CTG
Cuts at: 2190 2522 2919 3567 5139 2190
Size: 332 397 648 1572 2875
Fragments arranged by size:
2875 1572 648 397 332
ApaI G_GGCC'C
Cuts at: 2776 2776
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 2897 3133 3359 3511 5620 1493
Size: 1404 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1404 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2580 2940 3149 1870
Size: 73 216 421 360 209 4545
Fragments arranged by size:
4545 421 360 216 209 73
BamHI G'GATC_C
Cuts at: 3021 3154 3521 3021
Size: 133 367 5324
Fragments arranged by size:
5324 367 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3145 4600 618
Size: 325 217 627 269 1089 1455 1842
Fragments arranged by size:
1842 1455 1089 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2776 3042 3143 1391
Size: 1385 266 101 4072
Fragments arranged by size:
4072 1385 266 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 928
Size: 971 260 4593
Fragments arranged by size:
4593 971 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2679 2713 4015 4049 1903
Size: 34 742 34 1302 34 3678
Fragments arranged by size:
3678 1302 742 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BfrBI ATG'CAT
Cuts at: 2973 2973
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2776 3885 5041 1790
Size: 986 1109 1156 2573
Fragments arranged by size:
2573 1156 1109 986
BmgBI CAC'GTC
Cuts at: 1318 2293 1318
Size: 975 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2617 3555 4513
Size: 416 53 83 399 24 1200 938 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1200 958 938 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 2744 4484 1239
Size: 1083 422 1740 2579
Fragments arranged by size:
2579 1740 1083 422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2634 2808 3615 493
Size: 711 1430 174 807 2702
Fragments arranged by size:
2702 1430 807 711 174
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5599
Fragments arranged by size:
5599 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2259 2303 2375 1186
Size: 1073 44 72 4635
Fragments arranged by size:
4635 1073 72 44
BseYI C'CCAG_C
Cuts at: 2183 2446 2660 5027 5523 2183
Size: 263 214 2367 496 2484
Fragments arranged by size:
2484 2367 496 263 214
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2214 2396 1163
Size: 573 478 182 4591
Fragments arranged by size:
4591 573 478 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3885 3970 5041 3885
Size: 85 1071 4668
Fragments arranged by size:
4668 1071 85
BsmI GAATG_Cn'
Cuts at: 2975 3335 3428 2975
Size: 360 93 5371
Fragments arranged by size:
5371 360 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2776 3042 3143 3885 3970 5041 1391
Size: 399 986 266 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 986 742 399 266 101 85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2651 2825 3179 844
Size: 1807 174 354 3489
Fragments arranged by size:
3489 1807 354 174
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2391 4319 4493 65
Size: 1183 1143 1928 174 1396
Fragments arranged by size:
1928 1396 1183 1143 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2224 4468 4655 1700
Size: 110 414 2244 187 2869
Fragments arranged by size:
2869 2244 414 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3090 794
Size: 2296 3528
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2660 2684 2660
Size: 24 5800
BstYI r'GATC_y
Cuts at: 1972 3021 3154 3521 3906 3923 5375 5461 5473
Size: 1049 133 367 385 17 1452 86 12
Cuts at: 5473 5819 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1049 385 367 346 133 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2480 2480
Size: 5824
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2487 3042 513
Size: 552 939 75 408 555 3295
Fragments arranged by size:
3295 939 555 552 408 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2085 487
Size: 683 56 859 4226
Fragments arranged by size:
4226 859 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 4134 4154 3340
Size: 794 20 5010
Fragments arranged by size:
5010 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2122 3271
Size: 54 1425 50 275 18 246 46 1149
Cuts at: 3271 4160 5789 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1149 889 275 246 54 50
46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2249 2352 2490 2600 3083 3754 1353
Size: 665 231 103 138 110 483 671 3423
Fragments arranged by size:
3423 671 665 483 231 138 110 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 4411 4785 4931 387
Size: 1064 138 510 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 510 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2322 2376 2445 2744 3887 4484 5271
Size: 393 690 54 69 299 1143 597 787
Cuts at: 5271 5539 1239
Size: 268 1524
Fragments arranged by size:
1524 1143 787 690 597 393 299 268
69 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2780 2780
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 2382 2443 2456 2772 2773 2382
Size: 61 13 316 1 5433
Fragments arranged by size:
5433 316 61 13 1
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 4971 1678
Size: 3293 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2448 2480 4465 4497 4539
Size: 32 135 32 876 32 1985 32 42
Cuts at: 4539 4571 1373
Size: 32 2626
Fragments arranged by size:
2626 1985 876 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4002
Fragments arranged by size:
4002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3782 4141 4300 518
Size: 648 1644 972 359 159 2042
Fragments arranged by size:
2042 1644 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2410 2450 2656 3240 3917 5065 5310 1606
Size: 804 40 206 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 677 584 245 206 40
NaeI GCC'GGC
Cuts at: 2226 2226
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 3850
Fragments arranged by size:
3850 939 552 483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2224 2224
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NsiI A_TGCA'T
Cuts at: 2975 2975
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2130 2776 2851 1348
Size: 782 646 75 4321
Fragments arranged by size:
4321 782 646 75
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 1108
Size: 1082 4742
PfoI T'CCnGG_A
Cuts at: 2277 2754 2277
Size: 477 5347
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PmlI CAC'GTG
Cuts at: 2634 2808 2634
Size: 174 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 2456
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2772 2772
Size: 5824
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 5792
PstI C_TGCA'G
Cuts at: 830 2215 2915 3020 3176 830
Size: 1385 700 105 156 3478
Fragments arranged by size:
3478 1385 700 156 105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2410 2656 2410
Size: 246 5578
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SapI GCTCTTCn'nnn_
Cuts at: 2249 2490 2249
Size: 241 5583
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2211 2890 2911 3001 3016 3172 4307
Size: 219 1166 679 21 90 15 156 1135
Cuts at: 4307 4988 5179 826
Size: 681 191 1471
Fragments arranged by size:
1471 1166 1135 681 679 219 191 156
90 21 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2786 1517
Size: 1269 4555
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 3049 3263 513
Size: 552 939 483 562 214 3074
Fragments arranged by size:
3074 939 562 552 483 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 4070 5782
Size: 274 80 33 51 1443 894 1199 1712
Cuts at: 5782 96
Size: 138
Fragments arranged by size:
1712 1443 1199 894 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI PacI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI