(Circular) (Six-base) MAPSORT of: pFN21AB6554.seq Check: 4584 from: 1 to: 5878
pFN21AB6554 5878 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2616 3221 1937
Size: 679 605 4594
Fragments arranged by size:
4594 679 605
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 3057 3941 5325 5593 1632
Size: 815 610 884 1384 268 1917
Fragments arranged by size:
1917 1384 884 815 610 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 2089 2347 2412 4777 5754 1203
Size: 886 258 65 2365 977 1327
Fragments arranged by size:
2365 1327 977 886 258 65
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 2317 2932 4607 2317
Size: 615 1675 3588
Fragments arranged by size:
3588 1675 615
AleI CACnn'nnGTG
Cuts at: 1166 2984 1166
Size: 1818 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2841 2873 2841
Size: 32 5846
AlwNI CAG_nnn'CTG
Cuts at: 3621 5193 3621
Size: 1572 4306
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2208 2263 3187 3413 3565 5674 1493
Size: 715 55 924 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 715 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3203 1870
Size: 73 121 1139 4545
Fragments arranged by size:
4545 1139 121 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3199 4654 618
Size: 325 217 627 1412 1455 1842
Fragments arranged by size:
1842 1455 1412 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3197 1391
Size: 1806 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 2497 2722 1548
Size: 949 225 4704
Fragments arranged by size:
4704 949 225
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3171 3295 3171
Size: 124 5754
Bme1580I G_kGCm'C
Cuts at: 1790 3939 5095 1790
Size: 2149 1156 2573
Fragments arranged by size:
2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2539 3609
Size: 416 53 83 399 24 666 456 1070
Cuts at: 3609 4567 442
Size: 958 1753
Fragments arranged by size:
1753 1070 958 666 456 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4538 1239
Size: 3299 2579
Bpu10I CC'TnA_GC
Cuts at: 2604 2604
Size: 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 4003 4868 5166 2198
Size: 1805 865 298 2910
Fragments arranged by size:
2910 1805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2350 3669 493
Size: 711 1146 1319 2702
Fragments arranged by size:
2702 1319 1146 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2644 2674 1178
Size: 30 165 30 1241 30 4382
Fragments arranged by size:
4382 1241 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2286 1186
Size: 1100 4778
BseYI C'CCAG_C
Cuts at: 5081 5577 5081
Size: 496 5382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2967 3939 4024 5095 2508
Size: 459 972 85 1071 3291
Fragments arranged by size:
3291 1071 972 459 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2508 2967 3197 3939 4024 5095 1391
Size: 399 718 459 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 718 459 399 230 85
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 2490 2500 3767 5497 2490
Size: 10 1267 1730 2871
Fragments arranged by size:
2871 1730 1267 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3166 3233 844
Size: 2322 67 3489
Fragments arranged by size:
3489 2322 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2964 4373 4547 65
Size: 1183 1716 1409 174 1396
Fragments arranged by size:
1716 1409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 2131 4682 2131
Size: 2551 3327
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2749
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2800 3208 3575 3960 3977 5429 5515 5527
Size: 828 408 367 385 17 1452 86 12
Cuts at: 5527 5873 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 828 408 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2889 2889
Size: 5878
BtgI C'CryG_G
Cuts at: 513 1065 2704 513
Size: 552 1639 3687
Fragments arranged by size:
3687 1639 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2857 487
Size: 683 56 1631 3508
Fragments arranged by size:
3508 1631 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 4188 4208 3394
Size: 794 20 5064
Fragments arranged by size:
5064 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2557 3325 4214
Size: 54 1425 50 275 18 727 768 889
Cuts at: 4214 5843 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 768 727 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 3057 3941 4538 5325 5593 1239
Size: 393 815 610 884 597 787 268 1524
Fragments arranged by size:
1524 884 815 787 610 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3105 3105
Size: 5878
EcoO109I rG'GnC_Cy
Cuts at: 2012 2276 2431 2600 2012
Size: 264 155 169 5290
Fragments arranged by size:
5290 264 169 155
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
EcoRV GAT'ATC
Cuts at: 2818 2818
Size: 5878
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 3051 3171 5025 1678
Size: 1373 120 1854 2531
Fragments arranged by size:
2531 1854 1373 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2260 2292 2303
Size: 32 135 32 497 32 159 32 11
Cuts at: 2303 2335 2918 2950 4519 4551 4593 4625 1373
Size: 32 583 32 1569 32 42 32 2626
Fragments arranged by size:
2626 1569 583 497 159 135 42 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 3222 3468 669
Size: 988 281 450 834 246 3079
Fragments arranged by size:
3079 988 834 450 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 2388 3468 2388
Size: 1080 4798
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 2372 3477 2372
Size: 1105 4773
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4992 5176 1305
Size: 3687 184 2007
Fragments arranged by size:
3687 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2559 10
Size: 54 1425 343 727 3329
Fragments arranged by size:
3329 1425 727 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 2709 2984 3836 4195 4354 518
Size: 648 1303 240 275 852 359 159 2042
Fragments arranged by size:
2042 1303 852 648 359 275 240 159
MspA1I CmG'CkG
Cuts at: 1606 2125 3158 3164 3294 3971 5119 5364 1606
Size: 519 1033 6 130 677 1148 245 2120
Fragments arranged by size:
2120 1148 1033 677 519 245 130 6
NcoI C'CATG_G
Cuts at: 513 1065 2704 513
Size: 552 1639 3687
Fragments arranged by size:
3687 1639 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2093 2416 3022 3236 4781 1132
Size: 97 864 323 606 214 1545 2229
Fragments arranged by size:
2229 1545 864 606 323 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 2089 2412 4777 2089
Size: 323 2365 3190
Fragments arranged by size:
3190 2365 323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2012 2276 2600 2012
Size: 264 324 5290
Fragments arranged by size:
5290 324 264
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3230 830
Size: 2400 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2125 3158 2125
Size: 1033 4845
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SanDI GG'GwC_CC
Cuts at: 2012 2012
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 3226 4361 5042 5233 826
Size: 219 2181 1135 681 191 1471
Fragments arranged by size:
2181 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2064 2213 3982 4883 5145 820
Size: 197 926 121 149 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197 149 121
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3022 3236 1132
Size: 1890 214 3774
Fragments arranged by size:
3774 1890 214
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 2894 1517
Size: 1377 4501
StyI C'CwwG_G
Cuts at: 513 1065 2334 2704 2742 2895 3094 3139 3317
Size: 552 1269 370 38 153 199 45 178
Cuts at: 3317 513
Size: 3074
Fragments arranged by size:
3074 1269 552 370 199 178 153 45
38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3905 3922 4075 4260 4705 325
Size: 1255 2325 17 153 185 445 1498
Fragments arranged by size:
2325 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4124 5836 96
Size: 274 80 33 51 1443 2147 1712 138
Fragments arranged by size:
2147 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 2064 1943
Size: 121 5757
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 3007 4007 3007
Size: 1000 4878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI