Restriction map for
pFN21AB6553
(Circular) (Six-base) MAPSORT of: pFN21AB6553.seq Check: 5093 from: 1 to: 5818
pFN21AB6553 5818 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2227 2787 4717 5694 1203
Size: 1024 560 1930 977 1327
Fragments arranged by size:
1930 1327 1024 977 560
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2798 3561 5133 2798
Size: 763 1572 3483
Fragments arranged by size:
3483 1572 763
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 3127 3353 3505 5614 1493
Size: 1634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2115 3033 3143 1870
Size: 73 154 18 918 110 4545
Fragments arranged by size:
4545 918 154 110 73 18
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 2650 2713 2742 2857
Size: 325 217 627 431 432 63 29 115
Cuts at: 2857 2902 2917 3139 4594 618
Size: 45 15 222 1455 1842
Fragments arranged by size:
1842 1455 627 432 431 325 222 217
115 63 45 29 15
BanII G_rGCy'C
Cuts at: 1391 2467 2601 2785 3137 1391
Size: 1076 134 184 352 4072
Fragments arranged by size:
4072 1076 352 184 134
BbeI G_GCGC'C
Cuts at: 2746 2921 2746
Size: 175 5643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 4522
Fragments arranged by size:
4522 971 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4009 4043 1903
Size: 34 50 34 1988 34 3678
Fragments arranged by size:
3678 1988 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BfrBI ATG'CAT
Cuts at: 2981 2981
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2208 2261 2303
Size: 107 122 71 631 605 536 53 42
Cuts at: 2303 2740 2763 4429 136
Size: 437 23 1666 1525
Fragments arranged by size:
1666 1525 631 605 536 437 122 107
71 53 42 23
BglII A'GATC_T
Cuts at: 5813 5813
Size: 5818
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2860 3034 3879 5035 1790
Size: 431 639 174 845 1156 2573
Fragments arranged by size:
2573 1156 845 639 431 174
BmgBI CAC'GTC
Cuts at: 1318 2230 1318
Size: 912 4906
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2224 3549 4507
Size: 416 53 83 399 24 807 1325 958
Cuts at: 4507 442
Size: 1753
Fragments arranged by size:
1753 1325 958 807 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2114 1056
Size: 1058 4760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4478 1239
Size: 3239 2579
Bpu10I CC'TnA_GC
Cuts at: 2162 2162
Size: 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2788 3609 493
Size: 711 1584 821 2702
Fragments arranged by size:
2702 1584 821 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2743 2755 2918
Size: 53 83 186 936 482 728 12 163
Cuts at: 2918 3625 4007 4642 275
Size: 707 382 635 1451
Fragments arranged by size:
1451 936 728 707 635 482 382 186
163 83 53 12
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2772 2802 1178
Size: 30 165 30 1369 30 4194
Fragments arranged by size:
4194 1369 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2371 3082 1186
Size: 1185 711 3922
Fragments arranged by size:
3922 1185 711
BseYI C'CCAG_C
Cuts at: 2054 2063 2289 2523 2541 2580 2853 3050 5021
Size: 9 226 234 18 39 273 197 1971
Cuts at: 5021 5517 2054
Size: 496 2355
Fragments arranged by size:
2355 1971 496 273 234 226 197 39
18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2894 2944 3061 1163
Size: 573 1158 50 117 3920
Fragments arranged by size:
3920 1158 573 117 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2314 2480 3268 4029 5057 1540
Size: 275 186 313 166 788 761 1028 2301
Fragments arranged by size:
2301 1028 788 761 313 275 186 166
BsiHKAI G_wGCw'C
Cuts at: 2685 3879 3964 5035 2685
Size: 1194 85 1071 3468
Fragments arranged by size:
3468 1194 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2467 2601 2685 2785 2860 3034
Size: 399 431 246 134 84 100 75 174
Cuts at: 3034 3137 3879 3964 5035 1391
Size: 103 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 431 399 246 174 134
103 100 85 84 75
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 844
Size: 2329 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2173 4313 4487 65
Size: 1183 925 2140 174 1396
Fragments arranged by size:
2140 1396 1183 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2202 2475 2479 4462 4649 1700
Size: 110 392 273 4 1983 187 2869
Fragments arranged by size:
2869 1983 392 273 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2407 2459 2011
Size: 396 52 5370
Fragments arranged by size:
5370 396 52
BstYI r'GATC_y
Cuts at: 1972 2973 3148 3515 3900 3917 5369 5455 5467
Size: 1001 175 367 385 17 1452 86 12
Cuts at: 5467 5813 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 385 367 346 175 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2265 2320 2452 513
Size: 552 1200 55 132 3879
Fragments arranged by size:
3879 1200 552 132 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2253 2262
Size: 54 1425 50 275 18 409 14 9
Cuts at: 2262 2397 2477 2802 2943 3099 3265 4154 5783
Size: 135 80 325 141 156 166 889 1629
Cuts at: 5783 8
Size: 43
Fragments arranged by size:
1629 1425 889 409 325 275 166 156
141 135 80 54 50 43 18 14
9
EagI C'GGCC_G
Cuts at: 1537 1812 2477 3265 1537
Size: 275 665 788 4090
Fragments arranged by size:
4090 788 665 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 3748 1353
Size: 1181 1214 3423
Fragments arranged by size:
3423 1214 1181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 4405 4779 4925 387
Size: 1064 138 546 2270 374 146 1280
Fragments arranged by size:
2270 1280 1064 546 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3881 4478 5265 5533 1239
Size: 393 2249 597 787 268 1524
Fragments arranged by size:
2249 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2910 3101 2910
Size: 191 5627
EcoO109I rG'GnC_Cy
Cuts at: 2101 2893 3059 2101
Size: 792 166 4860
Fragments arranged by size:
4860 792 166
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2132 2746 2921 4965 1678
Size: 454 614 175 2044 2531
Fragments arranged by size:
2531 2044 614 454 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3098 3130 4459 4491 4533
Size: 32 135 32 1526 32 1329 32 42
Cuts at: 4533 4565 1373
Size: 32 2626
Fragments arranged by size:
2626 1526 1329 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3016 748
Size: 2268 3550
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KasI G'GCGC_C
Cuts at: 2742 2917 2742
Size: 175 5643
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4932 5116 1305
Size: 3627 184 2007
Fragments arranged by size:
3627 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 2399 2804 2945 3101
Size: 54 1425 343 409 158 405 141 156
Cuts at: 3101 10
Size: 2727
Fragments arranged by size:
2727 1425 409 405 343 158 156 141
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2457 3776 4135 4294 518
Size: 648 843 448 1319 359 159 2042
Fragments arranged by size:
2042 1319 843 648 448 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2107 2293 2565 2798 2878 2958 3234
Size: 424 77 186 272 233 80 80 276
Cuts at: 3234 3911 5059 5304 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 424 276 272 245 233
186 80 80 77
NaeI GCC'GGC
Cuts at: 2204 2204
Size: 5818
NarI GG'CG_CC
Cuts at: 2743 2918 2743
Size: 175 5643
NcoI C'CATG_G
Cuts at: 513 1065 2452 513
Size: 552 1387 3879
Fragments arranged by size:
3879 1387 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 2202 2202
Size: 5818
NheI G'CTAG_C
Cuts at: 1052 2110 1052
Size: 1058 4760
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NsiI A_TGCA'T
Cuts at: 2983 2983
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 2374 3176 4721 1132
Size: 97 1145 802 1545 2229
Fragments arranged by size:
2229 1545 1145 802 97
PasI CC'CwG_GG
Cuts at: 1348 2601 2659 1348
Size: 1253 58 4507
Fragments arranged by size:
4507 1253 58
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2970 1108
Size: 1862 3956
PfoI T'CCnGG_A
Cuts at: 2691 2691
Size: 5818
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PmlI CAC'GTG
Cuts at: 2788 2788
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2893 3059 2101
Size: 792 166 4860
Fragments arranged by size:
4860 792 166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PstI C_TGCA'G
Cuts at: 830 2361 3170 830
Size: 1531 809 3478
Fragments arranged by size:
3478 1531 809
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 2293 2798 2878 2958 2293
Size: 505 80 80 5153
Fragments arranged by size:
5153 505 80 80
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SanDI GG'GwC_CC
Cuts at: 2101 2893 3059 2101
Size: 792 166 4860
Fragments arranged by size:
4860 792 166
SapI GCTCTTCn'nnn_
Cuts at: 2534 2534
Size: 5818
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 2357 3166 4301 4982 5173 826
Size: 219 1312 809 1135 681 191 1471
Fragments arranged by size:
1471 1312 1135 809 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2208 2261 2303 2208
Size: 53 42 5723
Fragments arranged by size:
5723 53 42
SfoI GGC'GCC
Cuts at: 2744 2919 2744
Size: 175 5643
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 2099 2117 3035 3145 1872
Size: 227 18 918 110 4545
Fragments arranged by size:
4545 918 227 110 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3922 4823 5085 820
Size: 197 926 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SrfI GCCC'GGGC
Cuts at: 2117 2117
Size: 5818
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 2734 1517
Size: 1217 4601
StyI C'CwwG_G
Cuts at: 513 1065 2452 3102 3257 513
Size: 552 1387 650 155 3074
Fragments arranged by size:
3074 1387 650 552 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 3064 3845 3862 4015 4200 4645
Size: 1255 656 828 781 17 153 185 445
Cuts at: 4645 325
Size: 1498
Fragments arranged by size:
1498 1255 828 781 656 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4064 5776 96
Size: 274 80 33 51 1443 2087 1712 138
Fragments arranged by size:
2087 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2772 2804 2850 2882 5778 5810 2772
Size: 32 46 32 2896 32 2780
Fragments arranged by size:
2896 2780 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 3094 2146
Size: 948 4870
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5818
XmaI C'CCGG_G
Cuts at: 1870 2097 2115 3033 3143 1870
Size: 227 18 918 110 4545
Fragments arranged by size:
4545 918 227 110 18
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI PacI PspOMI PspXI PsrI
RsrII SacI SacII SexAI SwaI