Restriction map for
pFN21AB6545
(Circular) (Six-base) MAPSORT of: pFN21AB6545.seq Check: 8513 from: 1 to: 5800
pFN21AB6545 5800 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 3863 5247 5515 1632
Size: 467 1764 1384 268 1917
Fragments arranged by size:
1917 1764 1384 467 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2660 2786 3109 3335 3487 5596 1493
Size: 1167 126 323 226 152 2109 1697
Fragments arranged by size:
2109 1697 1167 323 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 2562 2945 4354 160
Size: 872 1530 383 1409 1606
Fragments arranged by size:
1606 1530 1409 872 383
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 3125 1870
Size: 73 158 1024 4545
Fragments arranged by size:
4545 1024 158 73
BamHI G'GATC_C
Cuts at: 2969 3130 3497 2969
Size: 161 367 5272
Fragments arranged by size:
5272 367 161
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2717 3121 4576 618
Size: 325 217 627 930 404 1455 1842
Fragments arranged by size:
1842 1455 930 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 2892 928
Size: 971 371 622 3836
Fragments arranged by size:
3836 971 622 371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3991 4025 1903
Size: 34 50 34 1970 34 3678
Fragments arranged by size:
3678 1970 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2685 1548
Size: 1137 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2295 3217 2295
Size: 922 4878
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 2913 1318
Size: 1595 4205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2890 3531 4489
Size: 416 53 83 399 24 1473 641 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1473 958 641 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2452 2880 3098 4460 1239
Size: 1106 107 428 218 1362 2579
Fragments arranged by size:
2579 1362 1106 428 218 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2293 3925 4790 5088 2030
Size: 263 1632 865 298 2742
Fragments arranged by size:
2742 1632 865 298 263
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2470 2765 4463 882
Size: 617 971 295 1698 2219
Fragments arranged by size:
2219 1698 971 617 295
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2750 2780 1178
Size: 30 165 30 1347 30 4198
Fragments arranged by size:
4198 1347 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2033 2564 2758 1186
Size: 838 9 531 194 4228
Fragments arranged by size:
4228 838 531 194 9
BseYI C'CCAG_C
Cuts at: 2008 2607 5003 5499 2008
Size: 599 2396 496 2309
Fragments arranged by size:
2396 2309 599 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2873 3861 3946 5017 2873
Size: 988 85 1071 3656
Fragments arranged by size:
3656 1071 988 85
BsmI GAATG_Cn'
Cuts at: 2653 3311 3404 2653
Size: 658 93 5049
Fragments arranged by size:
5049 658 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2873 3119 3861 3946 5017 1391
Size: 399 1083 246 742 85 1071 2174
Fragments arranged by size:
2174 1083 1071 742 399 246 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2591 2932 3155 844
Size: 1747 341 223 3489
Fragments arranged by size:
3489 1747 341 223
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2154 2485 2593 4295 4469 65
Size: 1183 906 331 108 1702 174 1396
Fragments arranged by size:
1702 1396 1183 906 331 174 108
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2837 3860 4872 2837
Size: 1023 1012 3765
Fragments arranged by size:
3765 1023 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2067 794
Size: 1273 4527
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 2020 2329 1184
Size: 836 309 4655
Fragments arranged by size:
4655 836 309
BstYI r'GATC_y
Cuts at: 1972 2421 2815 2969 3130 3497 3882 3899 5351
Size: 449 394 154 161 367 385 17 1452
Cuts at: 5351 5437 5449 5795 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 449 394 385 367 346 161
154 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2672 3247 4136
Size: 54 1425 50 275 18 842 575 889
Cuts at: 4136 5765 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 842 575 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2972 3730 1353
Size: 765 854 758 3423
Fragments arranged by size:
3423 854 765 758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2345 2452 2880 3098 3863 4460
Size: 393 467 246 107 428 218 765 597
Cuts at: 4460 5247 5515 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 765 597 467 428 393 268
246 218 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2546 2546
Size: 5800
EcoRI G'AATT_C
Cuts at: 2660 3109 3487 2660
Size: 449 378 4973
Fragments arranged by size:
4973 449 378
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 4441 4473 4515
Size: 32 135 32 1178 32 1659 32 42
Cuts at: 4515 4547 1373
Size: 32 2626
Fragments arranged by size:
2626 1659 1178 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2149 2557 3035 3144 3390 669
Size: 988 281 211 408 478 109 246 3079
Fragments arranged by size:
3079 988 478 408 281 246 211 109
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3758 4117 4276 518
Size: 648 2592 359 159 2042
Fragments arranged by size:
2592 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 3216 3893 5041 5286 1606
Size: 475 1135 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 677 475 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PfoI T'CCnGG_A
Cuts at: 2424 2857 2957 2972 2424
Size: 433 100 15 5252
Fragments arranged by size:
5252 433 100 15
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2522 2554 2522
Size: 32 5768
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2081 2081
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2405 3148 4283 4964 5155 826
Size: 219 1360 743 1135 681 191 1471
Fragments arranged by size:
1471 1360 1135 743 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2272 3904 4805 5067 820
Size: 197 926 102 227 1632 901 262 1553
Fragments arranged by size:
1632 1553 926 901 262 227 197 102
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2713 2781 3239 513
Size: 552 1648 68 458 3074
Fragments arranged by size:
3074 1648 552 458 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2978 3010 5760 5792 2978
Size: 32 2750 32 2986
Fragments arranged by size:
2986 2750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI