(Circular) (Six-base) MAPSORT of: pFN21AB6542.seq Check: 1305 from: 1 to: 5878 pFN21AB6542 5878 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2442 3221 1937 Size: 505 779 4594 Fragments arranged by size: 4594 779 505 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3941 5325 5593 1632 Size: 2309 1384 268 1917 Fragments arranged by size: 2309 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 2256 1166 Size: 1090 4788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 3621 5193 3621 Size: 1572 4306 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2290 2576 2659 3187 3413 3565 5674 1493 Size: 797 286 83 528 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 528 286 226 152 83 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2265 3203 1870 Size: 73 322 938 4545 Fragments arranged by size: 4545 938 322 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3199 4654 618 Size: 325 217 627 1412 1455 1842 Fragments arranged by size: 1842 1455 1412 627 325 217 BanII G_rGCy'C Cuts at: 1391 2100 2418 2832 3197 1391 Size: 709 318 414 365 4072 Fragments arranged by size: 4072 709 414 365 318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BbvCI CC'TCA_GC Cuts at: 2409 2409 Size: 5878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2366 2400 4069 4103 1903 Size: 34 429 34 1669 34 3678 Fragments arranged by size: 3678 1669 429 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2206 3770 4980 2206 Size: 1564 1210 3104 Fragments arranged by size: 3104 1564 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 2821 3939 5095 1790 Size: 1031 1118 1156 2573 Fragments arranged by size: 2573 1156 1118 1031 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2535 2535 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2111 2543 2952 4538 1239 Size: 818 54 432 409 1586 2579 Fragments arranged by size: 2579 1586 818 432 409 54 Bpu10I CC'TnA_GC Cuts at: 2409 2409 Size: 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 4003 4868 5166 2105 Size: 1898 865 298 2817 Fragments arranged by size: 2817 1898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2506 4541 882 Size: 617 1007 2035 2219 Fragments arranged by size: 2219 2035 1007 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 3042 5872 3042 Size: 2830 3048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2426 1186 Size: 922 318 4638 Fragments arranged by size: 4638 922 318 BseYI C'CCAG_C Cuts at: 5081 5577 5081 Size: 496 5382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 1163 Size: 573 297 5008 Fragments arranged by size: 5008 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2379 2418 3939 4024 5095 2100 Size: 279 39 1521 85 1071 2883 Fragments arranged by size: 2883 1521 1071 279 85 39 BsmI GAATG_Cn' Cuts at: 2400 3389 3482 2400 Size: 989 93 4796 Fragments arranged by size: 4796 989 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2379 2418 2821 2832 3197 3939 Size: 399 310 279 39 403 11 365 742 Cuts at: 3939 4024 5095 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 365 310 279 85 39 11 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2524 2858 3233 844 Size: 1680 334 375 3489 Fragments arranged by size: 3489 1680 375 334 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 4522 4709 1700 Size: 110 301 2411 187 2869 Fragments arranged by size: 2869 2411 301 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 2126 3938 4950 2126 Size: 1812 1012 3054 Fragments arranged by size: 3054 1812 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 3058 3058 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2130 3208 3575 3960 3977 5429 5515 5527 Size: 158 1078 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1078 385 367 346 158 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2143 2835 513 Size: 552 939 139 692 3556 Fragments arranged by size: 3556 939 692 552 139 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 2263 2317 2538 2930 3394 4188 4208 2263 Size: 54 221 392 464 794 20 3933 Fragments arranged by size: 3933 794 464 392 221 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 3325 4214 Size: 54 1425 50 275 18 316 1179 889 Cuts at: 4214 5843 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1179 889 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2098 2416 2098 Size: 318 5560 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2111 2543 2952 3941 4538 5325 Size: 393 425 54 432 409 989 597 787 Cuts at: 5325 5593 1239 Size: 268 1524 Fragments arranged by size: 1524 989 787 597 432 425 409 393 268 54 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 EcoRV GAT'ATC Cuts at: 2788 2788 Size: 5878 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2990 2990 Size: 5878 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2882 5025 1678 Size: 1204 2143 2531 Fragments arranged by size: 2531 2143 1204 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373 Size: 32 135 32 2947 32 42 32 2626 Fragments arranged by size: 2947 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2251 2259 4992 5176 1305 Size: 946 8 2733 184 2007 Fragments arranged by size: 2733 2007 946 184 8 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 3836 4195 4354 518 Size: 648 1090 1580 359 159 2042 Fragments arranged by size: 2042 1580 1090 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 3294 3971 5119 5364 1606 Size: 468 1220 677 1148 245 2120 Fragments arranged by size: 2120 1220 1148 677 468 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 NdeI CA'TA_TG Cuts at: 387 2752 387 Size: 2365 3513 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 3236 4781 1132 Size: 97 2007 1545 2229 Fragments arranged by size: 2229 2007 1545 97 PacI TTA_AT'TAA Cuts at: 2895 2895 Size: 5878 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 3151 3151 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2448 1613 Size: 835 5043 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 2139 3230 830 Size: 1309 1091 3478 Fragments arranged by size: 3478 1309 1091 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5878 SacI G_AGCT'C Cuts at: 2100 2418 2100 Size: 318 5560 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 2135 2439 3226 4361 5042 5233 826 Size: 219 1090 304 787 1135 681 191 1471 Fragments arranged by size: 1471 1135 1090 787 681 304 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 2111 1700 Size: 411 5467 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 3982 4883 5145 820 Size: 197 926 177 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 177 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 2484 3802 5 Size: 47 2432 1318 2081 Fragments arranged by size: 2432 2081 1318 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2004 2571 2974 3317 513 Size: 552 939 567 403 343 3074 Fragments arranged by size: 3074 939 567 552 403 343 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2606 3064 3905 3922 4075 4260 4705 Size: 1255 1026 458 841 17 153 185 445 Cuts at: 4705 325 Size: 1498 Fragments arranged by size: 1498 1255 1026 841 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2927 4124 5836 Size: 274 80 33 51 1443 950 1197 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1712 1443 1197 950 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2703 2735 5838 5870 2703 Size: 32 3103 32 2711 Fragments arranged by size: 3103 2711 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI