Restriction map for
pFN21AB6542
(Circular) (Six-base) MAPSORT of: pFN21AB6542.seq Check: 1305 from: 1 to: 5878
pFN21AB6542 5878 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2442 3221 1937
Size: 505 779 4594
Fragments arranged by size:
4594 779 505
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3941 5325 5593 1632
Size: 2309 1384 268 1917
Fragments arranged by size:
2309 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 2256 1166
Size: 1090 4788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 3621 5193 3621
Size: 1572 4306
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2290 2576 2659 3187 3413 3565 5674 1493
Size: 797 286 83 528 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 528 286 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 3203 1870
Size: 73 322 938 4545
Fragments arranged by size:
4545 938 322 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3199 4654 618
Size: 325 217 627 1412 1455 1842
Fragments arranged by size:
1842 1455 1412 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 2418 2832 3197 1391
Size: 709 318 414 365 4072
Fragments arranged by size:
4072 709 414 365 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BbvCI CC'TCA_GC
Cuts at: 2409 2409
Size: 5878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2366 2400 4069 4103 1903
Size: 34 429 34 1669 34 3678
Fragments arranged by size:
3678 1669 429 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2206 3770 4980 2206
Size: 1564 1210 3104
Fragments arranged by size:
3104 1564 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 2821 3939 5095 1790
Size: 1031 1118 1156 2573
Fragments arranged by size:
2573 1156 1118 1031
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2535 2535
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2111 2543 2952 4538 1239
Size: 818 54 432 409 1586 2579
Fragments arranged by size:
2579 1586 818 432 409 54
Bpu10I CC'TnA_GC
Cuts at: 2409 2409
Size: 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 4003 4868 5166 2105
Size: 1898 865 298 2817
Fragments arranged by size:
2817 1898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2506 4541 882
Size: 617 1007 2035 2219
Fragments arranged by size:
2219 2035 1007 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 3042 5872 3042
Size: 2830 3048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2426 1186
Size: 922 318 4638
Fragments arranged by size:
4638 922 318
BseYI C'CCAG_C
Cuts at: 5081 5577 5081
Size: 496 5382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 1163
Size: 573 297 5008
Fragments arranged by size:
5008 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2379 2418 3939 4024 5095 2100
Size: 279 39 1521 85 1071 2883
Fragments arranged by size:
2883 1521 1071 279 85 39
BsmI GAATG_Cn'
Cuts at: 2400 3389 3482 2400
Size: 989 93 4796
Fragments arranged by size:
4796 989 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2379 2418 2821 2832 3197 3939
Size: 399 310 279 39 403 11 365 742
Cuts at: 3939 4024 5095 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 365 310 279
85 39 11
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2524 2858 3233 844
Size: 1680 334 375 3489
Fragments arranged by size:
3489 1680 375 334
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 4522 4709 1700
Size: 110 301 2411 187 2869
Fragments arranged by size:
2869 2411 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 2126 3938 4950 2126
Size: 1812 1012 3054
Fragments arranged by size:
3054 1812 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 3058 3058
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2130 3208 3575 3960 3977 5429 5515 5527
Size: 158 1078 367 385 17 1452 86 12
Cuts at: 5527 5873 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1078 385 367 346 158 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2143 2835 513
Size: 552 939 139 692 3556
Fragments arranged by size:
3556 939 692 552 139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 2263 2317 2538 2930 3394 4188 4208 2263
Size: 54 221 392 464 794 20 3933
Fragments arranged by size:
3933 794 464 392 221 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 3325 4214
Size: 54 1425 50 275 18 316 1179 889
Cuts at: 4214 5843 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1179 889 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3808 1353
Size: 2455 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 2416 2098
Size: 318 5560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2111 2543 2952 3941 4538 5325
Size: 393 425 54 432 409 989 597 787
Cuts at: 5325 5593 1239
Size: 268 1524
Fragments arranged by size:
1524 989 787 597 432 425 409 393
268 54
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
EcoRV GAT'ATC
Cuts at: 2788 2788
Size: 5878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2990 2990
Size: 5878
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2882 5025 1678
Size: 1204 2143 2531
Fragments arranged by size:
2531 2143 1204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373
Size: 32 135 32 2947 32 42 32 2626
Fragments arranged by size:
2947 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2251 2259 4992 5176 1305
Size: 946 8 2733 184 2007
Fragments arranged by size:
2733 2007 946 184 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 3836 4195 4354 518
Size: 648 1090 1580 359 159 2042
Fragments arranged by size:
2042 1580 1090 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 3294 3971 5119 5364 1606
Size: 468 1220 677 1148 245 2120
Fragments arranged by size:
2120 1220 1148 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4387
Fragments arranged by size:
4387 939 552
NdeI CA'TA_TG
Cuts at: 387 2752 387
Size: 2365 3513
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 3236 4781 1132
Size: 97 2007 1545 2229
Fragments arranged by size:
2229 2007 1545 97
PacI TTA_AT'TAA
Cuts at: 2895 2895
Size: 5878
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 3151 3151
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2448 1613
Size: 835 5043
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 2139 3230 830
Size: 1309 1091 3478
Fragments arranged by size:
3478 1309 1091
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5878
SacI G_AGCT'C
Cuts at: 2100 2418 2100
Size: 318 5560
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 2135 2439 3226 4361 5042 5233 826
Size: 219 1090 304 787 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 787 681 304 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 2111 1700
Size: 411 5467
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 3982 4883 5145 820
Size: 197 926 177 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 2484 3802 5
Size: 47 2432 1318 2081
Fragments arranged by size:
2432 2081 1318 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2004 2571 2974 3317 513
Size: 552 939 567 403 343 3074
Fragments arranged by size:
3074 939 567 552 403 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2606 3064 3905 3922 4075 4260 4705
Size: 1255 1026 458 841 17 153 185 445
Cuts at: 4705 325
Size: 1498
Fragments arranged by size:
1498 1255 1026 841 458 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2927 4124 5836
Size: 274 80 33 51 1443 950 1197 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
1712 1443 1197 950 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2703 2735 5838 5870 2703
Size: 32 3103 32 2711
Fragments arranged by size:
3103 2711 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI