Restriction map for
pFN21AB6541
(Circular) (Six-base) MAPSORT of: pFN21AB6541.seq Check: 1223 from: 1 to: 5788
pFN21AB6541 5788 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2659 2810 3131 1937
Size: 722 151 321 4594
Fragments arranged by size:
4594 722 321 151
Acc65I G'GTAC_C
Cuts at: 2394 3109 2394
Size: 715 5073
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 2246 2805 4687 5664 1203
Size: 1043 559 1882 977 1327
Fragments arranged by size:
1882 1327 1043 977 559
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2259 1166
Size: 1093 4695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaI G_GGCC'C
Cuts at: 2867 2867
Size: 5788
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2962 3031 3097 3323 3475 5584 1493
Size: 1469 69 66 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152 69 66
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 3113 1870
Size: 73 115 1055 4545
Fragments arranged by size:
4545 1055 115 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2378 2411 2378
Size: 33 5755
BamHI G'GATC_C
Cuts at: 2485 3118 3485 2485
Size: 633 367 4788
Fragments arranged by size:
4788 633 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2394 2557 3109 4564 618
Size: 325 217 627 607 163 552 1455 1842
Fragments arranged by size:
1842 1455 627 607 552 325 217 163
BanII G_rGCy'C
Cuts at: 1391 2110 2867 3107 1391
Size: 719 757 240 4072
Fragments arranged by size:
4072 757 719 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BbvCI CC'TCA_GC
Cuts at: 2704 2704
Size: 5788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3680 4890 2418
Size: 1262 1210 3316
Fragments arranged by size:
3316 1262 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2562 2867 3002 3849 5005 1790
Size: 772 305 135 847 1156 2573
Fragments arranged by size:
2573 1156 847 772 305 135
BmgBI CAC'GTC
Cuts at: 1318 2240 1318
Size: 922 4866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2298 2469 3519
Size: 416 53 83 399 24 881 171 1050
Cuts at: 3519 4477 442
Size: 958 1753
Fragments arranged by size:
1753 1050 958 881 416 399 171 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2556 3079 2556
Size: 523 5265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 2421 2718 4448 1239
Size: 975 207 297 1730 2579
Fragments arranged by size:
2579 1730 975 297 207
Bpu10I CC'TnA_GC
Cuts at: 2704 2935 2704
Size: 231 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2242 3913 4778 5076 2242
Size: 1671 865 298 2954
Fragments arranged by size:
2954 1671 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2587 4451 882
Size: 617 1088 1864 2219
Fragments arranged by size:
2219 1864 1088 617
BsaAI yAC'GTr
Cuts at: 493 1204 2080 3579 493
Size: 711 876 1499 2702
Fragments arranged by size:
2702 1499 876 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2532 2692 1186
Size: 1346 160 4282
Fragments arranged by size:
4282 1346 160
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2244 1163
Size: 573 508 4707
Fragments arranged by size:
4707 573 508
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2110 3849 3934 5005 2110
Size: 1739 85 1071 2893
Fragments arranged by size:
2893 1739 1071 85
BsmI GAATG_Cn'
Cuts at: 2611 2631 2827 3299 3392 2611
Size: 20 196 472 93 5007
Fragments arranged by size:
5007 472 196 93 20
BsmBI CGTCTCn'nnnn_
Cuts at: 2230 2230
Size: 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2562 2867 3002 3107 3849 3934
Size: 399 320 452 305 135 105 742 85
Cuts at: 3934 5005 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 320 305 135
105 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 844
Size: 2299 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2595 4283 4457 65
Size: 1183 1347 1688 174 1396
Fragments arranged by size:
1688 1396 1347 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 2482
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2212 2485 3118 3485 3870 3887 5339 5425
Size: 240 273 633 367 385 17 1452 86
Cuts at: 5425 5437 5783 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 633 385 367 346 273 240
86 17 12
BstZ17I GTA'TAC
Cuts at: 2811 2811
Size: 5788
Bsu36I CC'TnA_GG
Cuts at: 2563 2563
Size: 5788
BtgI C'CryG_G
Cuts at: 513 1065 2883 513
Size: 552 1818 3418
Fragments arranged by size:
3418 1818 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3066 3101 529
Size: 35 2502 35 3216
Fragments arranged by size:
3216 2502 35 35
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2666 3235 4124
Size: 54 1425 50 275 18 836 569 889
Cuts at: 4124 5753 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 836 569 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3718 1353
Size: 2365 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2108 2108
Size: 5788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2421 2718 3851 4448 5235 5503
Size: 393 582 207 297 1133 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 1133 787 597 582 393 297 268
207
EcoNI CCTnn'n_nnAGG
Cuts at: 2547 2736 2547
Size: 189 5599
EcoO109I rG'GnC_Cy
Cuts at: 2351 2412 2729 2864 2351
Size: 61 317 135 5275
Fragments arranged by size:
5275 317 135 61
EcoRI G'AATT_C
Cuts at: 2962 3097 3475 2962
Size: 135 378 5275
Fragments arranged by size:
5275 378 135
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2037 4935 1678
Size: 359 2898 2531
Fragments arranged by size:
2898 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2416 2448 4429 4461 4503
Size: 32 135 32 844 32 1981 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 1981 844 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 2398 3113 2398
Size: 715 5073
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 2513 4902 5086 1305
Size: 765 443 2389 184 2007
Fragments arranged by size:
2389 2007 765 443 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2668 10
Size: 54 1425 343 836 3130
Fragments arranged by size:
3130 1425 836 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 2919 3077 3746 4105 4264 518
Size: 648 1093 660 158 669 359 159 2042
Fragments arranged by size:
2042 1093 669 660 648 359 159 158
MspA1I CmG'CkG
Cuts at: 1606 3204 3881 5029 5274 1606
Size: 1598 677 1148 245 2120
Fragments arranged by size:
2120 1598 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2883 513
Size: 552 1818 3418
Fragments arranged by size:
3418 1818 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2250 2344 2809 3146 4691 1132
Size: 97 1021 94 465 337 1545 2229
Fragments arranged by size:
2229 1545 1021 465 337 97 94
PasI CC'CwG_GG
Cuts at: 1348 2867 1348
Size: 1519 4269
PciI A'CATG_T
Cuts at: 2246 2805 4687 2246
Size: 559 1882 3347
Fragments arranged by size:
3347 1882 559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2336 2654 2741 1108
Size: 1228 318 87 4155
Fragments arranged by size:
4155 1228 318 87
PfoI T'CCnGG_A
Cuts at: 2734 2833 2734
Size: 99 5689
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2351 2729 2351
Size: 378 5410
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2863 2863
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2296 3140 830
Size: 1466 844 3478
Fragments arranged by size:
3478 1466 844
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
SacI G_AGCT'C
Cuts at: 2110 2110
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2292 3136 4271 4952 5143 826
Size: 219 1247 844 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 844 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 2060 3115 1872
Size: 188 1055 4545
Fragments arranged by size:
4545 1055 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2257 3892 4793 5055 820
Size: 197 926 314 1635 901 262 1553
Fragments arranged by size:
1635 1553 926 901 314 262 197
SnaBI TAC'GTA
Cuts at: 493 2080 3579 493
Size: 1587 1499 2702
Fragments arranged by size:
2702 1587 1499
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 2344 3146 1132
Size: 1212 802 3774
Fragments arranged by size:
3774 1212 802
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2408 2663 2883 3005 3227 513
Size: 552 1343 255 220 122 222 3074
Fragments arranged by size:
3074 1343 552 255 222 220 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 2776 2876 3815 3832 3985 4170
Size: 1255 927 269 100 939 17 153 185
Cuts at: 4170 4615 325
Size: 445 1498
Fragments arranged by size:
1498 1255 939 927 445 269 185 153
100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2058 3113 1870
Size: 188 1055 4545
Fragments arranged by size:
4545 1055 188
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI