Restriction map for
pFN21AB6540
(Circular) (Six-base) MAPSORT of: pFN21AB6540.seq Check: 8118 from: 1 to: 5878
pFN21AB6540 5878 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2235 2914 3941 5325 5593 1632
Size: 449 154 679 1027 1384 268 1917
Fragments arranged by size:
1917 1384 1027 679 449 268 154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2756 1184
Size: 1572 4306
AlwNI CAG_nnn'CTG
Cuts at: 3020 3621 5193 3020
Size: 601 1572 3705
Fragments arranged by size:
3705 1572 601
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2603 2697 3187 3413 3565 5674 1493
Size: 1110 94 490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1110 490 226 152 94
AseI AT'TA_AT
Cuts at: 160 1032 2448 4432 160
Size: 872 1416 1984 1606
Fragments arranged by size:
1984 1606 1416 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2953 3203 1870
Size: 73 196 814 250 4545
Fragments arranged by size:
4545 814 250 196 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 3029 3199 4654 618
Size: 325 217 627 231 1011 170 1455 1842
Fragments arranged by size:
1842 1455 1011 627 325 231 217 170
BanII G_rGCy'C
Cuts at: 1391 2201 3197 1391
Size: 810 996 4072
Fragments arranged by size:
4072 996 810
BbeI G_GCGC'C
Cuts at: 2022 2022
Size: 5878
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BfrBI ATG'CAT
Cuts at: 2393 2393
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 3939 5095 1790
Size: 2149 1156 2573
Fragments arranged by size:
2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 3609 4567
Size: 416 53 83 399 24 735 1457 958
Cuts at: 4567 442
Size: 1753
Fragments arranged by size:
1753 1457 958 735 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 2789 4538 1239
Size: 1460 90 1749 2579
Fragments arranged by size:
2579 1749 1460 90
Bpu10I CC'TnA_GC
Cuts at: 2729 2729
Size: 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 4003 4868 5166 2185
Size: 1818 865 298 2897
Fragments arranged by size:
2897 1818 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 3685 4067 4702
Size: 53 83 186 936 486 1666 382 635
Cuts at: 4702 275
Size: 1451
Fragments arranged by size:
1666 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2186 1178
Size: 30 165 30 753 30 4870
Fragments arranged by size:
4870 753 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2157 2180 1186
Size: 971 23 4884
Fragments arranged by size:
4884 971 23
BseYI C'CCAG_C
Cuts at: 5081 5577 5081
Size: 496 5382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2837 2981 1163
Size: 573 1101 144 4060
Fragments arranged by size:
4060 1101 573 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2201 2624 2940 3939 4024 5095 2201
Size: 423 316 999 85 1071 2984
Fragments arranged by size:
2984 1071 999 423 316 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
BsmBI CGTCTCn'nnnn_
Cuts at: 2183 2183
Size: 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2624 2940 3197 3939 4024 5095
Size: 399 411 423 316 257 742 85 1071
Cuts at: 5095 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 423 411 399 316 257
85
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 3085 3767 5497 3085
Size: 682 1730 3466
Fragments arranged by size:
3466 1730 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3233 844
Size: 2389 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2567 4373 4547 65
Size: 1183 1319 1806 174 1396
Fragments arranged by size:
1806 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 2635 96
Size: 2539 3339
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2917 2917
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873
Size: 1236 367 385 17 1452 86 12 346
Cuts at: 5873 1972
Size: 1977
Fragments arranged by size:
1977 1452 1236 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 3481
Fragments arranged by size:
3481 939 906 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2937 487
Size: 683 56 1711 3428
Fragments arranged by size:
3428 1711 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3394 4188 4208 3097
Size: 297 794 20 4767
Fragments arranged by size:
4767 794 297 20
ClaI AT'CG_AT
Cuts at: 1554 2760 1554
Size: 1206 4672
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 2554 3182 2554
Size: 628 5250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3325 4214 5843
Size: 54 1425 50 275 18 1495 889 1629
Cuts at: 5843 8
Size: 43
Fragments arranged by size:
1629 1495 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 2354 2592 3158 3808 1353
Size: 836 165 238 566 650 3423
Fragments arranged by size:
3423 836 650 566 238 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3064 4465 4839 4985 387
Size: 1064 138 1475 1401 374 146 1280
Fragments arranged by size:
1475 1401 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2199 2199
Size: 5878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2235 2699 2789 2914 3941 4538
Size: 393 449 154 464 90 125 1027 597
Cuts at: 4538 5325 5593 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1027 787 597 464 449 393 268
154 125 90
EcoO109I rG'GnC_Cy
Cuts at: 2136 2136
Size: 5878
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2022 5025 1678
Size: 344 3003 2531
Fragments arranged by size:
3003 2531 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373
Size: 32 135 32 2947 32 42 32 2626
Fragments arranged by size:
2947 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KasI G'GCGC_C
Cuts at: 2018 2018
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 2115 3477 2115
Size: 1362 4516
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 3165 4992 5176 1305
Size: 837 1023 1827 184 2007
Fragments arranged by size:
2007 1827 1023 837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 2915 3090 3836 4195 4354 518
Size: 648 1572 177 175 746 359 159 2042
Fragments arranged by size:
2042 1572 746 648 359 177 175 159
MspA1I CmG'CkG
Cuts at: 1606 3044 3294 3971 5119 5364 1606
Size: 1438 250 677 1148 245 2120
Fragments arranged by size:
2120 1438 1148 677 250 245
NarI GG'CG_CC
Cuts at: 2019 2019
Size: 5878
NcoI C'CATG_G
Cuts at: 513 1065 2004 2910 513
Size: 552 939 906 3481
Fragments arranged by size:
3481 939 906 552
NdeI CA'TA_TG
Cuts at: 387 2829 387
Size: 2442 3436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NsiI A_TGCA'T
Cuts at: 2395 2395
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2737 3236 4781 1132
Size: 97 1508 499 1545 2229
Fragments arranged by size:
2229 1545 1508 499 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2676 2676
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2136 2136
Size: 5878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3043 3230 830
Size: 2213 187 3478
Fragments arranged by size:
3478 2213 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 3044 3044
Size: 5878
SacI G_AGCT'C
Cuts at: 2201 2201
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SanDI GG'GwC_CC
Cuts at: 2136 2136
Size: 5878
SapI GCTCTTCn'nnn_
Cuts at: 2189 2189
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 3039 3226 4361 5042 5233 826
Size: 219 1994 187 1135 681 191 1471
Fragments arranged by size:
1994 1471 1135 681 219 191 187
SfoI GGC'GCC
Cuts at: 2020 2020
Size: 5878
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2200 3982 4883 5145 820
Size: 197 926 257 1782 901 262 1553
Fragments arranged by size:
1782 1553 926 901 262 257 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 2816 152
Size: 2664 3214
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 2384 3802 5
Size: 47 2332 1418 2081
Fragments arranged by size:
2332 2081 1418 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2004 2910 3317 513
Size: 552 939 906 407 3074
Fragments arranged by size:
3074 939 906 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3905 3922 4075 4260 4705 325
Size: 1255 2325 17 153 185 445 1498
Fragments arranged by size:
2325 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 2635 4124
Size: 274 80 33 51 1443 271 387 1489
Cuts at: 4124 5836 96
Size: 1712 138
Fragments arranged by size:
1712 1489 1443 387 274 271 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3122 3154 5838 5870 3122
Size: 32 2684 32 3130
Fragments arranged by size:
3130 2684 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 2890 3214 2890
Size: 324 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 3093 4007 3093
Size: 914 4964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI