Restriction map for
pFN21AB6537
(Circular) (Six-base) MAPSORT of: pFN21AB6537.seq Check: 1208 from: 1 to: 5806
pFN21AB6537 5806 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3869 5253 5521 1632
Size: 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 268
AfeI AGC'GCT
Cuts at: 2111 2399 2987 2111
Size: 288 588 4930
Fragments arranged by size:
4930 588 288
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2130 3087 1184
Size: 946 957 3903
Fragments arranged by size:
3903 957 946
AlwNI CAG_nnn'CTG
Cuts at: 3549 5121 3549
Size: 1572 4234
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 2789 2855 3115 3341 3493 5602 1493
Size: 1296 66 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 1296 260 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 2468 2850 3131 1870
Size: 73 340 185 382 281 4545
Fragments arranged by size:
4545 382 340 281 185 73
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3127 4582 618
Size: 325 217 627 269 1071 1455 1842
Fragments arranged by size:
1842 1455 1071 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2290 2355 2694 2963 3125 1391
Size: 899 65 339 269 162 4072
Fragments arranged by size:
4072 899 339 269 162 65
BbeI G_GCGC'C
Cuts at: 2060 2060
Size: 5806
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3108 928
Size: 971 1209 3626
Fragments arranged by size:
3626 1209 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4417 136
Size: 107 122 71 631 605 2745 1525
Fragments arranged by size:
2745 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 3867 5023 1790
Size: 2077 1156 2573
Fragments arranged by size:
2573 2077 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3043 4466 1239
Size: 1804 1423 2579
Fragments arranged by size:
2579 1804 1423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2322 2428 3597 493
Size: 711 1118 106 1169 2702
Fragments arranged by size:
2702 1169 1118 711 106
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 3613 3995 4630
Size: 53 83 186 936 524 1556 382 635
Cuts at: 4630 275
Size: 1451
Fragments arranged by size:
1556 1451 936 635 524 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2487 2887 4239 4637 4911 5058 5469 2487
Size: 400 1352 398 274 147 411 2824
Fragments arranged by size:
2824 1352 411 400 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 2831 2861 1178
Size: 30 165 30 1362 30 36 30 4123
Fragments arranged by size:
4123 1362 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2855 1186
Size: 873 796 4137
Fragments arranged by size:
4137 873 796
BseYI C'CCAG_C
Cuts at: 2963 3046 5009 5505 2963
Size: 83 1963 496 3264
Fragments arranged by size:
3264 1963 496 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2136 1163
Size: 573 390 10 4833
Fragments arranged by size:
4833 573 390 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2290 2355 2694 2899 3867 3952 5023 2290
Size: 65 339 205 968 85 1071 3073
Fragments arranged by size:
3073 1071 968 339 205 85 65
BsmI GAATG_Cn'
Cuts at: 2452 3317 3410 2452
Size: 865 93 4848
Fragments arranged by size:
4848 865 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2290 2355 2694 2899 2963 3125 3867
Size: 399 500 65 339 205 64 162 742
Cuts at: 3867 3952 5023 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 399 339 205 162
85 65 64
BspEI T'CCGG_A
Cuts at: 2887 5469 2887
Size: 2582 3224
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 3161 844
Size: 1829 488 3489
Fragments arranged by size:
3489 1829 488
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4450 4637 1700
Size: 110 2640 187 2869
Fragments arranged by size:
2869 2640 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801
Size: 1164 367 385 17 1452 86 12 346
Cuts at: 5801 1972
Size: 1977
Fragments arranged by size:
1977 1452 1164 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2350 2416 2542 3322 4116 4136 2124
Size: 226 66 126 780 794 20 3794
Fragments arranged by size:
3794 794 780 226 126 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2478 3253 4142
Size: 54 1425 50 275 18 648 775 889
Cuts at: 4142 5771 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 775 648 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 3736 1353
Size: 683 1700 3423
Fragments arranged by size:
3423 1700 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2674 4393 4767 4913 387
Size: 1064 138 1085 1719 374 146 1280
Fragments arranged by size:
1719 1280 1085 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2288 2353 2692 2288
Size: 65 339 5402
Fragments arranged by size:
5402 339 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3043 3869 4466 5253 5521 1239
Size: 393 1411 826 597 787 268 1524
Fragments arranged by size:
1524 1411 826 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2182 2182
Size: 5806
EcoRI G'AATT_C
Cuts at: 2855 3115 3493 2855
Size: 260 378 5168
Fragments arranged by size:
5168 378 260
EcoRV GAT'ATC
Cuts at: 3085 3085
Size: 5806
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2060 2113 2401 2989 4953 1678
Size: 382 53 288 588 1964 2531
Fragments arranged by size:
2531 1964 588 382 288 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2765 2797 2831 2863 4447
Size: 32 135 32 1193 32 34 32 1584
Cuts at: 4447 4479 4521 4553 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 1193 135 42 34 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2743 3150 3396 669
Size: 988 281 226 579 407 246 3079
Fragments arranged by size:
3079 988 579 407 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 2056 2056
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2606 4920 5104 1305
Size: 1301 2314 184 2007
Fragments arranged by size:
2314 2007 1301 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3764 4123 4282 518
Size: 648 1215 1383 359 159 2042
Fragments arranged by size:
2042 1383 1215 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3222 3899 5047 5292 1606
Size: 1616 677 1148 245 2120
Fragments arranged by size:
2120 1616 1148 677 245
NarI GG'CG_CC
Cuts at: 2057 2057
Size: 5806
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
NdeI CA'TA_TG
Cuts at: 387 2907 387
Size: 2520 3286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NruI TCG'CGA
Cuts at: 2982 2982
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2575 3164 4709 1132
Size: 97 1346 589 1545 2229
Fragments arranged by size:
2229 1545 1346 589 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5806
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 2550 3376 2550
Size: 826 4980
PstI C_TGCA'G
Cuts at: 830 2670 2940 3076 3158 830
Size: 1840 270 136 82 3478
Fragments arranged by size:
3478 1840 270 136 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
SacI G_AGCT'C
Cuts at: 2290 2355 2694 2290
Size: 65 339 5402
Fragments arranged by size:
5402 339 65
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2666 2936 3072 3154 4289 4970 5161
Size: 219 1621 270 136 82 1135 681 191
Cuts at: 5161 826
Size: 1471
Fragments arranged by size:
1621 1471 1135 681 270 219 191 136
82
SfoI GGC'GCC
Cuts at: 2058 2058
Size: 5806
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 3133 1872
Size: 1261 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 4811 5073 820
Size: 197 926 1967 901 262 1553
Fragments arranged by size:
1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2322 3597 493
Size: 1829 1275 2702
Fragments arranged by size:
2702 1829 1275
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 2256 3730 5
Size: 47 2204 1474 2081
Fragments arranged by size:
2204 2081 1474 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 3245 513
Size: 552 939 21 1220 3074
Fragments arranged by size:
3074 1220 939 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2457 2489 5766 5798 2000
Size: 32 425 32 3277 32 2008
Fragments arranged by size:
3277 2008 425 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2408 1316
Size: 1092 4714
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 3131 1870
Size: 1261 4545
XmnI GAAnn'nnTTC
Cuts at: 2309 2793 3935 2309
Size: 484 1142 4180
Fragments arranged by size:
4180 1142 484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I FalI FseI FspAI NaeI NgoMIV NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI