Restriction map for
pFN21AB6535
(Circular) (Six-base) MAPSORT of: pFN21AB6535.seq Check: 4711 from: 1 to: 5806
pFN21AB6535 5806 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2276 2276
Size: 5806
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 3149 1937
Size: 451 761 4594
Fragments arranged by size:
4594 761 451
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 3869 5253 5521 1632
Size: 707 1530 1384 268 1917
Fragments arranged by size:
1917 1530 1384 707 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 2291 2480 2816 3029 1166
Size: 1125 189 336 213 3943
Fragments arranged by size:
3943 1125 336 213 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2758 3047 1184
Size: 1574 289 3943
Fragments arranged by size:
3943 1574 289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 5774
AlwNI CAG_nnn'CTG
Cuts at: 2114 2615 3549 5121 2114
Size: 501 934 1572 2799
Fragments arranged by size:
2799 1572 934 501
ApaLI G'TGCA_C
Cuts at: 2486 3863 5019 2486
Size: 1377 1156 3273
Fragments arranged by size:
3273 1377 1156
ApoI r'AATT_y
Cuts at: 1493 2556 3115 3341 3493 5602 1493
Size: 1063 559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1063 559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2562 3131 1870
Size: 73 619 569 4545
Fragments arranged by size:
4545 619 569 73
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2792 3127 4582 618
Size: 325 217 627 1005 335 1455 1842
Fragments arranged by size:
1842 1455 1005 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2569 2774 3125 1391
Size: 1178 205 351 4072
Fragments arranged by size:
4072 1178 351 205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BbvCI CC'TCA_GC
Cuts at: 2214 2214
Size: 5806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2162 3698 4908 2162
Size: 1536 1210 3060
Fragments arranged by size:
3060 1536 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2648 4417 136
Size: 107 122 71 631 605 976 1769 1525
Fragments arranged by size:
1769 1525 976 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 2037 2568 3223 2037
Size: 531 655 4620
Fragments arranged by size:
4620 655 531
Bme1580I G_kGCm'C
Cuts at: 1790 2490 2580 3867 5023 1790
Size: 700 90 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 700 90
BmgBI CAC'GTC
Cuts at: 1318 2428 1318
Size: 1110 4696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2856 2901 4466 1239
Size: 1331 286 45 1565 2579
Fragments arranged by size:
2579 1565 1331 286 45
Bpu10I CC'TnA_GC
Cuts at: 2214 2214
Size: 5806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2980 4469 882
Size: 617 1481 1489 2219
Fragments arranged by size:
2219 1489 1481 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3613 3995 4630 275
Size: 53 83 186 936 2080 382 635 1451
Fragments arranged by size:
2080 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2225 4239 4637 4911 5058 5469 2225
Size: 2014 398 274 147 411 2562
Fragments arranged by size:
2562 2014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 2850 2880 1178
Size: 30 165 30 1116 30 301 30 4104
Fragments arranged by size:
4104 1116 301 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2904 1186
Size: 1718 4088
BseYI C'CCAG_C
Cuts at: 3056 5009 5505 3056
Size: 1953 496 3357
Fragments arranged by size:
3357 1953 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3053 1163
Size: 573 1317 3916
Fragments arranged by size:
3916 1317 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2490 2774 3867 3952 5023 2490
Size: 284 1093 85 1071 3273
Fragments arranged by size:
3273 1093 1071 284 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 2569 2580 2774 3125 3867 3952
Size: 399 700 79 11 194 351 742 85
Cuts at: 3952 5023 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 700 399 351 194 85
79 11
BspEI T'CCGG_A
Cuts at: 2225 5469 2225
Size: 3244 2562
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2276 2673 2750 2958 3161 844
Size: 1432 397 77 208 203 3489
Fragments arranged by size:
3489 1432 397 208 203 77
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4450 4637 1700
Size: 110 2640 187 2869
Fragments arranged by size:
2869 2640 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2439 2732 794
Size: 1645 293 3868
Fragments arranged by size:
3868 1645 293
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 2985 1184
Size: 1801 4005
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2818 2293
Size: 525 5281
BstYI r'GATC_y
Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801
Size: 1164 367 385 17 1452 86 12 346
Cuts at: 5801 1972
Size: 1977
Fragments arranged by size:
1977 1452 1164 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2389 2389
Size: 5806
Bsu36I CC'TnA_GG
Cuts at: 2992 2992
Size: 5806
BtgI C'CryG_G
Cuts at: 513 1065 2382 2817 513
Size: 552 1317 435 3502
Fragments arranged by size:
3502 1317 552 435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2115 487
Size: 683 56 889 4178
Fragments arranged by size:
4178 889 683 56
BtsI GCAGTG_nn'
Cuts at: 2742 3322 4116 4136 2742
Size: 580 794 20 4412
Fragments arranged by size:
4412 794 580 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DraIII CAC_nnn'GTG
Cuts at: 2708 2708
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 3253 4142
Size: 54 1425 50 275 18 678 745 889
Cuts at: 4142 5771 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 745 678 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3736 1353
Size: 2383 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2772 2772
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2570 2856 2901 3869 4466 5253
Size: 393 707 231 286 45 968 597 787
Cuts at: 5253 5521 1239
Size: 268 1524
Fragments arranged by size:
1524 968 787 707 597 393 286 268
231 45
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 2432 4312 2432
Size: 1880 3926
HaeII r_GCGC'y
Cuts at: 1678 4953 1678
Size: 3275 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4447 4479 4521 4553 1373
Size: 32 135 32 2875 32 42 32 2626
Fragments arranged by size:
2875 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2714 748
Size: 1966 3840
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2480 2816 2963 2969 3029 3764
Size: 648 1125 189 336 147 6 60 735
Cuts at: 3764 4123 4282 518
Size: 359 159 2042
Fragments arranged by size:
2042 1125 735 648 359 336 189 159
147 60 6
MspA1I CmG'CkG
Cuts at: 1606 2317 2356 2467 2548 2876 3222 3899 5047
Size: 711 39 111 81 328 346 677 1148
Cuts at: 5047 5292 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 711 677 346 328 245 111
81 39
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2330 3164 4709 1132
Size: 97 1101 834 1545 2229
Fragments arranged by size:
2229 1545 1101 834 97
PasI CC'CwG_GG
Cuts at: 1348 2072 1348
Size: 724 5082
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2278 1108
Size: 1170 4636
PfoI T'CCnGG_A
Cuts at: 2917 2917
Size: 5806
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2551 3875 3907 5388 5420 2519
Size: 32 1324 32 1481 32 2905
Fragments arranged by size:
2905 1481 1324 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PstI C_TGCA'G
Cuts at: 830 3158 830
Size: 2328 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2317 2356 2548 2876 2317
Size: 39 192 328 5247
Fragments arranged by size:
5247 328 192 39
SacI G_AGCT'C
Cuts at: 2774 2774
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2136 3154 4289 4970 5161 826
Size: 219 1091 1018 1135 681 191 1471
Fragments arranged by size:
1471 1135 1091 1018 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2564 3133 1872
Size: 692 569 4545
Fragments arranged by size:
4545 692 569
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 4811 5073 820
Size: 197 926 1967 901 262 1553
Fragments arranged by size:
1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 2330 3164 1132
Size: 1198 834 3774
Fragments arranged by size:
3774 1198 834
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2162 3245 513
Size: 552 1097 1083 3074
Fragments arranged by size:
3074 1097 1083 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2957 3833 3850 4003 4188 4633 325
Size: 1255 1377 876 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 876 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2850 2882 5766 5798 2850
Size: 32 2884 32 2858
Fragments arranged by size:
2884 2858 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2545 2818 2545
Size: 273 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2562 3131 1870
Size: 692 569 4545
Fragments arranged by size:
4545 692 569
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI