Restriction map for
pFN21AB6533
(Circular) (Six-base) MAPSORT of: pFN21AB6533.seq Check: 3087 from: 1 to: 5806
pFN21AB6533 5806 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2423 3149 1937
Size: 486 726 4594
Fragments arranged by size:
4594 726 486
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2884 3059 3869 5253 5521 1632
Size: 499 753 175 810 1384 268 1917
Fragments arranged by size:
1917 1384 810 753 499 268 175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 2156 3014 1166
Size: 990 858 3958
Fragments arranged by size:
3958 990 858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2174 2600 1184
Size: 990 426 4390
Fragments arranged by size:
4390 990 426
AlwNI CAG_nnn'CTG
Cuts at: 2493 2709 2920 2941 2966 3549 5121 2493
Size: 216 211 21 25 583 1572 3178
Fragments arranged by size:
3178 1572 583 216 211 25 21
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 3131 1870
Size: 73 1188 4545
Fragments arranged by size:
4545 1188 73
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2163 2177 2237 2288
Size: 325 217 627 234 142 14 60 51
Cuts at: 2288 2303 2999 3127 4582 618
Size: 15 696 128 1455 1842
Fragments arranged by size:
1842 1455 696 627 325 234 217 142
128 60 51 15 14
BanII G_rGCy'C
Cuts at: 1391 2217 2439 2975 3083 3125 1391
Size: 826 222 536 108 42 4072
Fragments arranged by size:
4072 826 536 222 108 42
BbeI G_GCGC'C
Cuts at: 2167 2181 2241 2292 2167
Size: 14 60 51 5681
Fragments arranged by size:
5681 60 51 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 928
Size: 971 451 4384
Fragments arranged by size:
4384 971 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2369 3698 4908 2369
Size: 1329 1210 3267
Fragments arranged by size:
3267 1329 1210
BclI T'GATC_A
Cuts at: 1548 2613 1548
Size: 1065 4741
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 2784 4417
Size: 107 122 71 631 605 646 466 1633
Cuts at: 4417 136
Size: 1525
Fragments arranged by size:
1633 1525 646 631 605 466 122 107
71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 2438 3065 3223 2438
Size: 627 158 5021
Fragments arranged by size:
5021 627 158
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2205 3867 5023 1790
Size: 234 181 1662 1156 2573
Fragments arranged by size:
2573 1662 1156 234 181
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2210 2775 4466 1239
Size: 816 155 565 1691 2579
Fragments arranged by size:
2579 1691 816 565 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2164 2178 2238 2289
Size: 53 83 186 936 631 14 60 51
Cuts at: 2289 3613 3995 4630 275
Size: 1324 382 635 1451
Fragments arranged by size:
1451 1324 936 635 631 382 186 83
60 53 51 14
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 2690 2720 2748
Size: 30 165 30 675 30 582 30 28
Cuts at: 2748 2778 3079 3109 1178
Size: 30 301 30 3875
Fragments arranged by size:
3875 675 582 301 165 30 30 30
30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5806
BseYI C'CCAG_C
Cuts at: 2053 2966 3098 5009 5505 2053
Size: 913 132 1911 496 2354
Fragments arranged by size:
2354 1911 913 496 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2431 2506 1163
Size: 573 695 75 4463
Fragments arranged by size:
4463 695 573 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 2322 2995 3256 4017 5045
Size: 275 186 223 98 673 261 761 1028
Cuts at: 5045 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 673 275 261 223 186
98
BsiHKAI G_wGCw'C
Cuts at: 2196 2217 2991 3867 3952 5023 2196
Size: 21 774 876 85 1071 2979
Fragments arranged by size:
2979 1071 876 774 85 21
BsmI GAATG_Cn'
Cuts at: 2755 3317 3410 2755
Size: 562 93 5151
Fragments arranged by size:
5151 562 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2626 3037 2626
Size: 411 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2196 2205 2217 2439 2975 2991
Size: 399 234 172 9 12 222 536 16
Cuts at: 2991 3083 3125 3867 3952 5023 1391
Size: 92 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 536 399 234 222 172
92 85 42 16 12 9
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2066 2094 3693 1394
Size: 429 243 28 1599 3507
Fragments arranged by size:
3507 1599 429 243 28
BsrDI GCAATG_nn'
Cuts at: 65 1248 2078 4301 4475 65
Size: 1183 830 2223 174 1396
Fragments arranged by size:
2223 1396 1183 830 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2126 2234 2321 4450 4637 1700
Size: 110 304 12 108 87 2129 187 2869
Fragments arranged by size:
2869 2129 304 187 110 108 87 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 2325 2342 2344 1925
Size: 400 17 2 5387
Fragments arranged by size:
5387 400 17 2
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 2200 2493 794
Size: 1283 123 293 4107
Fragments arranged by size:
4107 1283 293 123
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2188 2011
Size: 177 5629
BstYI r'GATC_y
Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801
Size: 1164 367 385 17 1452 86 12 346
Cuts at: 5801 1972
Size: 1977
Fragments arranged by size:
1977 1452 1164 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2218 2250 2430 2979 513
Size: 552 939 177 37 32 180 549 3340
Fragments arranged by size:
3340 939 552 549 180 177 37 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2107 487
Size: 683 56 881 4186
Fragments arranged by size:
4186 881 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 2773 3322 4116 4136 2563
Size: 210 549 794 20 4233
Fragments arranged by size:
4233 794 549 210 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DraIII CAC_nnn'GTG
Cuts at: 2200 2251 2200
Size: 51 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2221 2232
Size: 54 1425 50 275 18 286 105 11
Cuts at: 2232 2319 3253 4142 5771 8
Size: 87 934 889 1629 43
Fragments arranged by size:
1629 1425 934 889 286 275 105 87
54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2221 2319 3253 1537
Size: 275 409 98 934 4090
Fragments arranged by size:
4090 934 409 275 98
EarI CTCTTCn'nnn_
Cuts at: 1353 3736 1353
Size: 2383 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2131 2210 2775 2884 3059 3869
Size: 393 423 76 79 565 109 175 810
Cuts at: 3869 4466 5253 5521 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 565 423 393 268
175 109 79 76
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2051 2167 2181 2241 2277 2292 4953 1678
Size: 373 116 14 60 36 15 2661 2531
Fragments arranged by size:
2661 2531 373 116 60 36 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2690 2722 2818
Size: 32 135 32 506 32 580 32 96
Cuts at: 2818 2850 4447 4479 4521 4553 1373
Size: 32 1597 32 42 32 2626
Fragments arranged by size:
2626 1597 580 506 135 96 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 2424 3150 3396 669
Size: 988 281 199 287 726 246 3079
Fragments arranged by size:
3079 988 726 287 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 2163 2177 2237 2288 2163
Size: 14 60 51 5681
Fragments arranged by size:
5681 60 51 14
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 2629 3405 2629
Size: 776 5030
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2015 2156 2675 2984 3014 3764
Size: 648 843 6 141 519 309 30 750
Cuts at: 3764 4123 4282 518
Size: 359 159 2042
Fragments arranged by size:
2042 843 750 648 519 359 309 159
141 30 6
MspA1I CmG'CkG
Cuts at: 1606 2220 2917 3102 3222 3899 5047 5292 1606
Size: 614 697 185 120 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 614 245 185 120
NaeI GCC'GGC
Cuts at: 2116 2128 2236 2323 2116
Size: 12 108 87 5599
Fragments arranged by size:
5599 108 87 12
NarI GG'CG_CC
Cuts at: 2164 2178 2238 2289 2164
Size: 14 60 51 5681
Fragments arranged by size:
5681 60 51 14
NcoI C'CATG_G
Cuts at: 513 1065 2004 2181 2430 2979 513
Size: 552 939 177 249 549 3340
Fragments arranged by size:
3340 939 552 549 249 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 2114 2126 2234 2321 2114
Size: 12 108 87 5599
Fragments arranged by size:
5599 108 87 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 2221 3253 2221
Size: 1032 4774
NspI r_CATG'y
Cuts at: 1132 1229 2587 3164 4709 1132
Size: 97 1358 577 1545 2229
Fragments arranged by size:
2229 1545 1358 577 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 2941 1108
Size: 1143 690 3973
Fragments arranged by size:
3973 1143 690
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2498 2925 3158 830
Size: 1668 427 233 3478
Fragments arranged by size:
3478 1668 427 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2917 3102 2917
Size: 185 5621
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 5806
SacII CC_GC'GG
Cuts at: 2221 2221
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 2422 3148 1936
Size: 486 726 4594
Fragments arranged by size:
4594 726 486
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 2455 4054 5766 2455
Size: 1599 1712 2495
Fragments arranged by size:
2495 1712 1599
SfcI C'TryA_G
Cuts at: 826 1045 2494 2921 3154 4289 4970 5161 826
Size: 219 1449 427 233 1135 681 191 1471
Fragments arranged by size:
1471 1449 1135 681 427 233 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2318 2318
Size: 5806
SfoI GGC'GCC
Cuts at: 2165 2179 2239 2290 2165
Size: 14 60 51 5681
Fragments arranged by size:
5681 60 51 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 3133 1872
Size: 1261 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 4811 5073 820
Size: 197 926 1967 901 262 1553
Fragments arranged by size:
1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 2040 2855 1517
Size: 523 815 4468
Fragments arranged by size:
4468 815 523
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2181 2430 2595 2979 3245
Size: 552 939 168 9 249 165 384 266
Cuts at: 3245 513
Size: 3074
Fragments arranged by size:
3074 939 552 384 266 249 168 165
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2472 3009 3833 3850 4003 4188 4633
Size: 1255 892 537 824 17 153 185 445
Cuts at: 4633 325
Size: 1498
Fragments arranged by size:
1498 1255 892 824 537 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2453 2661 4052
Size: 274 80 33 51 1443 476 208 1391
Cuts at: 4052 5764 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1391 476 274 208 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 2748 2780 5766 5798 2468
Size: 32 248 32 2986 32 2476
Fragments arranged by size:
2986 2476 248 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2179
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 3131 1870
Size: 1261 4545
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI
SexAI SrfI SwaI