Restriction map for
pFN21AB6532
(Circular) (Six-base) MAPSORT of: pFN21AB6532.seq Check: 8092 from: 1 to: 5872
pFN21AB6532 5872 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2257 2616 3215 1937
Size: 320 359 599 4594
Fragments arranged by size:
4594 599 359 320
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 2771 3999 4372 1563
Size: 1208 1228 373 3063
Fragments arranged by size:
3063 1228 1208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2015 2165 2220 2300 2730 3069 3935 5319
Size: 383 150 55 80 430 339 866 1384
Cuts at: 5319 5587 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 866 430 383 339 268 150
80 55
AflII C'TTAA_G
Cuts at: 820 1017 2081 820
Size: 197 1064 4611
Fragments arranged by size:
4611 1064 197
AflIII A'CryG_T
Cuts at: 1203 2367 4771 5748 1203
Size: 1164 2404 977 1327
Fragments arranged by size:
2404 1327 1164 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 1184
Size: 882 4990
AlwNI CAG_nnn'CTG
Cuts at: 3615 5187 3615
Size: 1572 4300
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2207 3181 3407 3559 5668 1493
Size: 714 974 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 714 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 3197 1870
Size: 73 1254 4545
Fragments arranged by size:
4545 1254 73
BamHI G'GATC_C
Cuts at: 2350 2860 3202 3569 2350
Size: 510 342 367 4653
Fragments arranged by size:
4653 510 367 342
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 4648 618
Size: 325 217 627 1406 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3191 1391
Size: 1800 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3105 928
Size: 971 1206 3695
Fragments arranged by size:
3695 1206 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 2646 2829 3076 1548
Size: 1098 183 247 4344
Fragments arranged by size:
4344 1098 247 183
BfrBI ATG'CAT
Cuts at: 2688 2688
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3028 5867 3028
Size: 2839 3033
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 3933 5089 1790
Size: 2143 1156 2573
Fragments arranged by size:
2573 2143 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2571 1056
Size: 1515 4357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3164 4532 1239
Size: 1925 1368 2579
Fragments arranged by size:
2579 1925 1368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4862 5160 3997
Size: 865 298 4709
Fragments arranged by size:
4709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5647
Fragments arranged by size:
5647 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5872
BseYI C'CCAG_C
Cuts at: 5075 5571 5075
Size: 496 5376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 3322 4083 5111 1540
Size: 275 186 76 1245 761 1028 2301
Fragments arranged by size:
2301 1245 1028 761 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 2641 3933 4018 5089 2641
Size: 1292 85 1071 3424
Fragments arranged by size:
3424 1292 1071 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2641 3191 3933 4018 5089 1391
Size: 399 851 550 742 85 1071 2174
Fragments arranged by size:
2174 1071 851 742 550 399 85
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 2325 2374 3079 3105 3761 5491 2325
Size: 49 705 26 656 1730 2706
Fragments arranged by size:
2706 1730 705 656 49 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 3044 3227 844
Size: 2101 99 183 3489
Fragments arranged by size:
3489 2101 183 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4367 4541 65
Size: 1183 3119 174 1396
Fragments arranged by size:
3119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 2556 3932 4944 2556
Size: 1376 1012 3484
Fragments arranged by size:
3484 1376 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3071 794
Size: 2277 3595
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 2350 2427 2860 3028 3202 3569 3954 3971
Size: 378 77 433 168 174 367 385 17
Cuts at: 3971 5423 5509 5521 5867 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 433 385 378 367 346 174
168 86 77 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5133
Fragments arranged by size:
5133 683 56
BtsI GCAGTG_nn'
Cuts at: 3388 4182 4202 3388
Size: 794 20 5058
Fragments arranged by size:
5058 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 3319 4208
Size: 54 1425 50 275 18 244 1245 889
Cuts at: 4208 5837 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1245 889 275 244 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2074 3319 1537
Size: 275 262 1245 4090
Fragments arranged by size:
4090 1245 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 3802 1353
Size: 2449 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4459 4833 4979 387
Size: 1064 138 435 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2165 2220 2300 2730 3069 3164
Size: 393 383 150 55 80 430 339 95
Cuts at: 3164 3935 4532 5319 5587 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 597 430 393 383 339
268 150 95 80 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2505 2505
Size: 5872
EcoO109I rG'GnC_Cy
Cuts at: 2149 2380 2957 2149
Size: 231 577 5064
Fragments arranged by size:
5064 577 231
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2316 2316
Size: 5872
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 2030 5019 1678
Size: 352 2989 2531
Fragments arranged by size:
2989 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373
Size: 32 135 32 2941 32 42 32 2626
Fragments arranged by size:
2941 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2196 2258 3216 3462 669
Size: 988 281 258 62 958 246 3079
Fragments arranged by size:
3079 988 958 281 258 246 62
HindIII A'AGCT_T
Cuts at: 748 3002 748
Size: 2254 3618
HpaI GTT'AAC
Cuts at: 2196 3462 2196
Size: 1266 4606
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 2994 3471 2994
Size: 477 5395
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4986 5170 1305
Size: 3681 184 2007
Fragments arranged by size:
3681 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 3830 4189 4348 518
Size: 648 1530 1134 359 159 2042
Fragments arranged by size:
2042 1530 1134 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2575 2750 3288 3965 5113 5358 1606
Size: 969 175 538 677 1148 245 2120
Fragments arranged by size:
2120 1148 969 677 538 245 175
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 2567 1052
Size: 1515 4357
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NsiI A_TGCA'T
Cuts at: 2690 2690
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2371 3230 4775 1132
Size: 97 1142 859 1545 2229
Fragments arranged by size:
2229 1545 1142 859 97
PasI CC'CwG_GG
Cuts at: 1348 2384 1348
Size: 1036 4836
PciI A'CATG_T
Cuts at: 2367 4771 2367
Size: 2404 3468
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PfoI T'CCnGG_A
Cuts at: 2430 2878 2430
Size: 448 5424
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2957 2380
Size: 577 5295
PshAI GACnn'nnGTC
Cuts at: 1613 2076 1613
Size: 463 5409
PsiI TTA'TAA
Cuts at: 2364 3442 2364
Size: 1078 4794
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2575 2750 2575
Size: 175 5697
SalI G'TCGA_C
Cuts at: 1936 2256 3214 1936
Size: 320 958 4594
Fragments arranged by size:
4594 958 320
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SexAI A'CCwGG_T
Cuts at: 2530 2530
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 2153 3220 4355 5036 5227 826
Size: 219 1108 1067 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 1067 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 3976 4877 5139 820
Size: 197 926 138 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 197 138
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2004 3311 513
Size: 552 939 1307 3074
Fragments arranged by size:
3074 1307 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 3899 3916 4069 4254 4699 325
Size: 1255 585 1734 17 153 185 445 1498
Fragments arranged by size:
1734 1498 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 3044 3091
Size: 274 80 33 51 1443 645 422 47
Cuts at: 3091 4118 5830 96
Size: 1027 1712 138
Fragments arranged by size:
1712 1443 1027 645 422 274 138 80
51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5832 5864 5832
Size: 32 5840
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI