Restriction map for
pFN21AB6531
(Circular) (Six-base) MAPSORT of: pFN21AB6531.seq Check: 3266 from: 1 to: 5803
pFN21AB6531 5803 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3866 5250 5518 1632
Size: 2234 1384 268 1917
Fragments arranged by size:
2234 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AlwNI CAG_nnn'CTG
Cuts at: 2393 2503 2980 3546 5118 2393
Size: 110 477 566 1572 3078
Fragments arranged by size:
3078 1572 566 477 110
ApaI G_GGCC'C
Cuts at: 2237 2786 2879 2237
Size: 549 93 5161
Fragments arranged by size:
5161 549 93
ApaLI G'TGCA_C
Cuts at: 2678 3860 5016 2678
Size: 1182 1156 3465
Fragments arranged by size:
3465 1182 1156
ApoI r'AATT_y
Cuts at: 1493 2576 2812 3112 3338 3490 5599 1493
Size: 1083 236 300 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 300 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2700 3128 1870
Size: 73 757 428 4545
Fragments arranged by size:
4545 757 428 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2686 2867 3124 4579 618
Size: 325 217 627 899 181 257 1455 1842
Fragments arranged by size:
1842 1455 899 627 325 257 217 181
BanII G_rGCy'C
Cuts at: 1391 2237 2432 2756 2786 2861 2879 2907 3122
Size: 846 195 324 30 75 18 28 215
Cuts at: 3122 1391
Size: 4072
Fragments arranged by size:
4072 846 324 215 195 75 30 28
18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2141 2762 928
Size: 971 242 621 3969
Fragments arranged by size:
3969 971 621 242
BbvCI CC'TCA_GC
Cuts at: 2268 2463 2268
Size: 195 5608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 2954 3220 2954
Size: 266 5537
Bme1580I G_kGCm'C
Cuts at: 1790 2237 2682 2786 2872 2879 3070 3864 5020
Size: 447 445 104 86 7 191 794 1156
Cuts at: 5020 1790
Size: 2573
Fragments arranged by size:
2573 1156 794 447 445 191 104 86
7
BmgBI CAC'GTC
Cuts at: 1318 2929 1318
Size: 1611 4192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2396 3534 4492
Size: 416 53 83 399 24 979 1138 958
Cuts at: 4492 442
Size: 1753
Fragments arranged by size:
1753 1138 979 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2339 4463 1239
Size: 1088 12 2124 2579
Fragments arranged by size:
2579 2124 1088 12
Bpu10I CC'TnA_GC
Cuts at: 2268 2463 2268
Size: 195 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3928 4793 5091 3065
Size: 863 865 298 3777
Fragments arranged by size:
3777 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2138 3610 3992 4627
Size: 53 83 186 936 605 1472 382 635
Cuts at: 4627 275
Size: 1451
Fragments arranged by size:
1472 1451 936 635 605 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2371 4236 4634 4908 5055 5466 2371
Size: 1865 398 274 147 411 2708
Fragments arranged by size:
2708 1865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 1178
Size: 30 165 30 1320 30 4228
Fragments arranged by size:
4228 1320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2927 2963 1186
Size: 844 897 36 4026
Fragments arranged by size:
4026 897 844 36
BseYI C'CCAG_C
Cuts at: 2237 2997 5006 5502 2237
Size: 760 2009 496 2538
Fragments arranged by size:
2538 2009 760 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2206 2531 1163
Size: 573 470 325 4435
Fragments arranged by size:
4435 573 470 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2494 2682 2907 3864 3949 5020 2494
Size: 188 225 957 85 1071 3277
Fragments arranged by size:
3277 1071 957 225 188 85
BsmI GAATG_Cn'
Cuts at: 2656 3314 3407 2656
Size: 658 93 5052
Fragments arranged by size:
5052 658 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2432 2494 2682 2756 2786 2861
Size: 399 447 195 62 188 74 30 75
Cuts at: 2861 2872 2879 2907 3070 3122 3864 3949 5020
Size: 11 7 28 163 52 742 85 1071
Cuts at: 5020 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 447 399 195 188 163
85 75 74 62 52 30 28 11
7
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 844
Size: 2314 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2716 4298 4472 65
Size: 1183 1468 1582 174 1396
Fragments arranged by size:
1582 1468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2311 3001 4447 4634 1700
Size: 110 501 690 1446 187 2869
Fragments arranged by size:
2869 1446 690 501 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2393 2686 794
Size: 1599 293 3911
Fragments arranged by size:
3911 1599 293
BstBI TT'CG_AA
Cuts at: 2773 4607 2773
Size: 1834 3969
BstEII G'GTnAC_C
Cuts at: 1184 2200 1184
Size: 1016 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 2555 2638 2749 2555
Size: 83 111 5609
Fragments arranged by size:
5609 111 83
BstYI r'GATC_y
Cuts at: 1972 2829 3133 3500 3885 3902 5354 5440 5452
Size: 857 304 367 385 17 1452 86 12
Cuts at: 5452 5798 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 857 385 367 346 304 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2361 2361
Size: 5803
BtgI C'CryG_G
Cuts at: 513 1065 2037 2436 3046 513
Size: 552 972 399 610 3270
Fragments arranged by size:
3270 972 610 552 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 2354 3319 4113 4133 2354
Size: 965 794 20 4024
Fragments arranged by size:
4024 965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DraIII CAC_nnn'GTG
Cuts at: 2686 3012 2686
Size: 326 5477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2551 4810 809
Size: 507 1235 2259 1802
Fragments arranged by size:
2259 1802 1235 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2658 3003
Size: 54 1425 50 275 18 243 585 345
Cuts at: 3003 3250 4139 5768 8
Size: 247 889 1629 43
Fragments arranged by size:
1629 1425 889 585 345 275 247 243
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2356 3733 1353
Size: 654 349 1377 3423
Fragments arranged by size:
3423 1377 654 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2905 2905
Size: 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2339 3866 4463 5250 5518 1239
Size: 393 695 12 1527 597 787 268 1524
Fragments arranged by size:
1527 1524 787 695 597 393 268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 2108
Size: 5803
EcoO109I rG'GnC_Cy
Cuts at: 2233 2234 2782 2876 2233
Size: 1 548 94 5160
Fragments arranged by size:
5160 548 94 1
EcoRI G'AATT_C
Cuts at: 2576 3112 3490 2576
Size: 536 378 4889
Fragments arranged by size:
4889 536 378
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 2814 2846 4444
Size: 32 135 32 1151 32 59 32 1598
Cuts at: 4444 4476 4518 4550 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1598 1151 135 59 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 3761 4120 4279 518
Size: 648 1581 1014 359 159 2042
Fragments arranged by size:
2042 1581 1014 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 2267 2393 3219 3896 5044 5289 1606
Size: 614 47 126 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 677 614 245 126 47
NaeI GCC'GGC
Cuts at: 2313 3003 2313
Size: 690 5113
NcoI C'CATG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 3880
Fragments arranged by size:
3880 1371 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NgoMIV G'CCGG_C
Cuts at: 2311 3001 2311
Size: 690 5113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2305 3161 4706 1132
Size: 97 1076 856 1545 2229
Fragments arranged by size:
2229 1545 1076 856 97
PasI CC'CwG_GG
Cuts at: 1348 2854 2863 2872 1348
Size: 1506 9 9 4279
Fragments arranged by size:
4279 1506 9 9
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2980 1108
Size: 1872 3931
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2233 2782 2875 2233
Size: 549 93 5161
Fragments arranged by size:
5161 549 93
PstI C_TGCA'G
Cuts at: 830 2112 2389 2448 2499 3075 3155 830
Size: 1282 277 59 51 576 80 3478
Fragments arranged by size:
3478 1282 576 277 80 59 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2220 2267 2393 2220
Size: 47 126 5630
Fragments arranged by size:
5630 126 47
SacI G_AGCT'C
Cuts at: 2907 2907
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2108 2385 2412 2444 2495 3071 3151
Size: 219 1063 277 27 32 51 576 80
Cuts at: 3151 4286 4967 5158 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1063 681 576 277 219 191
80 51 32 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 2702 3130 1872
Size: 830 428 4545
Fragments arranged by size:
4545 830 428
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3080 3907 4808 5070 820
Size: 197 926 1137 827 901 262 1553
Fragments arranged by size:
1553 1137 926 901 827 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 2305 3161 1132
Size: 1173 856 3774
Fragments arranged by size:
3774 1173 856
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 2921 1517
Size: 1404 4399
StyI C'CwwG_G
Cuts at: 513 1065 2436 3242 513
Size: 552 1371 806 3074
Fragments arranged by size:
3074 1371 806 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2855 3011 3830 3847 4000 4185 4630
Size: 1255 1275 156 819 17 153 185 445
Cuts at: 4630 325
Size: 1498
Fragments arranged by size:
1498 1275 1255 819 445 185 156 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 4049 5761
Size: 274 80 33 51 1443 558 1514 1712
Cuts at: 5761 96
Size: 138
Fragments arranged by size:
1712 1514 1443 558 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5763 5795 5763
Size: 32 5771
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2741 2793 2741
Size: 52 5751
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 2700 3128 1870
Size: 830 428 4545
Fragments arranged by size:
4545 830 428
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI