Restriction map for
pFN21AB6530
(Circular) (Six-base) MAPSORT of: pFN21AB6530.seq Check: 4277 from: 1 to: 5542
pFN21AB6530 5542 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2129 2129
Size: 5542
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 278
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 1937
Size: 948 4594
Acc65I G'GTAC_C
Cuts at: 2336 2863 2336
Size: 527 5015
AclI AA'CG_TT
Cuts at: 1563 3669 4042 1563
Size: 2106 373 3063
Fragments arranged by size:
3063 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3605 4989 5257 1632
Size: 1973 1384 268 1917
Fragments arranged by size:
1973 1917 1384 268
AfeI AGC'GCT
Cuts at: 2015 2600 2015
Size: 585 4957
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5345
AflIII A'CryG_T
Cuts at: 1203 4441 5418 1203
Size: 3238 977 1327
Fragments arranged by size:
3238 1327 977
AgeI A'CCGG_T
Cuts at: 4373 4373
Size: 5542
AhdI GACnn_n'nnGTC
Cuts at: 4271 4271
Size: 5542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5542
AlwNI CAG_nnn'CTG
Cuts at: 2367 3285 4857 2367
Size: 918 1572 3052
Fragments arranged by size:
3052 1572 918
ApaLI G'TGCA_C
Cuts at: 2566 2674 3599 4755 2566
Size: 108 925 1156 3353
Fragments arranged by size:
3353 1156 925 108
ApoI r'AATT_y
Cuts at: 1493 2446 2851 3077 3229 5338 1493
Size: 953 405 226 152 2109 1697
Fragments arranged by size:
2109 1697 953 405 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4096 160
Size: 872 3064 1606
Fragments arranged by size:
3064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 1870
Size: 73 924 4545
Fragments arranged by size:
4545 924 73
BamHI G'GATC_C
Cuts at: 2872 3239 2872
Size: 367 5175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 2863 4318 618
Size: 325 217 627 549 527 1455 1842
Fragments arranged by size:
1842 1455 627 549 527 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2861 1391
Size: 690 780 4072
Fragments arranged by size:
4072 780 690
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 928
Size: 971 428 4143
Fragments arranged by size:
4143 971 428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2603 2637 3733 3767 1903
Size: 34 666 34 1096 34 3678
Fragments arranged by size:
3678 1096 666 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3434 4644 3434
Size: 1210 4332
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4153 136
Size: 107 122 71 631 605 2481 1525
Fragments arranged by size:
2481 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5537 5537
Size: 5542
BlpI GC'TnA_GC
Cuts at: 2959 2959
Size: 5542
Bme1580I G_kGCm'C
Cuts at: 1790 2460 2570 2678 2822 3603 4759 1790
Size: 670 110 108 144 781 1156 2573
Fragments arranged by size:
2573 1156 781 670 144 110 108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3273 4231 442
Size: 416 53 83 399 24 1856 958 1753
Fragments arranged by size:
1856 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4202 1239
Size: 2963 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3667 4532 4830 3667
Size: 865 298 4379
Fragments arranged by size:
4379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 2369 4205 882
Size: 617 597 273 1836 2219
Fragments arranged by size:
2219 1836 617 597 273
BsaAI yAC'GTr
Cuts at: 493 1204 3333 493
Size: 711 2129 2702
Fragments arranged by size:
2702 2129 711
BsaBI GATnn'nnATC
Cuts at: 5536 5536
Size: 5542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3349 3731 4366 275
Size: 53 83 186 936 1816 382 635 1451
Fragments arranged by size:
1816 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3975 4373 4647 4794 5205 3975
Size: 398 274 147 411 4312
Fragments arranged by size:
4312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5317
Fragments arranged by size:
5317 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2141 1186
Size: 901 54 4587
Fragments arranged by size:
4587 901 54
BseYI C'CCAG_C
Cuts at: 2669 4745 5241 2669
Size: 2076 496 2970
Fragments arranged by size:
2970 2076 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2236 1163
Size: 573 500 4469
Fragments arranged by size:
4469 573 500
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2992 3753 4781 1540
Size: 275 186 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2570 2678 2768 3603 3688 4759 2570
Size: 108 90 835 85 1071 3353
Fragments arranged by size:
3353 1071 835 108 90 85
BsmI GAATG_Cn'
Cuts at: 2318 3053 3146 2318
Size: 735 93 4714
Fragments arranged by size:
4714 735 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2460 2570 2678 2768 2822 2861
Size: 399 291 379 110 108 90 54 39
Cuts at: 2861 3603 3688 4759 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 291 110 108
90 85 54 39
BspEI T'CCGG_A
Cuts at: 5205 5205
Size: 5542
BspHI T'CATG_A
Cuts at: 3431 5161 3431
Size: 1730 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2129 2387 2897 844
Size: 1285 258 510 3489
Fragments arranged by size:
3489 1285 510 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3429 1394
Size: 429 1606 3507
Fragments arranged by size:
3507 1606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2029 4037 4211 65
Size: 1183 781 2008 174 1396
Fragments arranged by size:
2008 1396 1183 781 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 4186 4373 1700
Size: 110 368 2008 187 2869
Fragments arranged by size:
2869 2008 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 2270 96
Size: 2174 3368
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5542
BssSI C'ACGA_G
Cuts at: 3602 4614 3602
Size: 1012 4530
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2574 794
Size: 1780 3762
BstBI TT'CG_AA
Cuts at: 4346 4346
Size: 5542
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5542
BstYI r'GATC_y
Cuts at: 1972 2872 3239 3624 3641 5093 5179 5191 5537
Size: 900 367 385 17 1452 86 12 346
Cuts at: 5537 1972
Size: 1977
Fragments arranged by size:
1977 1452 900 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 3541
Fragments arranged by size:
3541 1449 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2628 487
Size: 683 56 1402 3401
Fragments arranged by size:
3401 1402 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2219 2492 2738 3058 3852 3872 2203
Size: 16 273 246 320 794 20 3873
Fragments arranged by size:
3873 794 320 273 246 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5507
DraI TTT'AAA
Cuts at: 2846 2846
Size: 5542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4549 809
Size: 507 3233 1802
Fragments arranged by size:
3233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2989 3878 5507
Size: 54 1425 50 275 18 1159 889 1629
Cuts at: 5507 8
Size: 43
Fragments arranged by size:
1629 1425 1159 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2989 1537
Size: 275 1177 4090
Fragments arranged by size:
4090 1177 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3472 1353
Size: 2119 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4129 4503 4649 387
Size: 1064 138 2540 374 146 1280
Fragments arranged by size:
2540 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3605 4202 4989 5257 1239
Size: 393 1973 597 787 268 1524
Fragments arranged by size:
1973 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2069
Size: 5542
EcoRI G'AATT_C
Cuts at: 2851 3229 2851
Size: 378 5164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 5542
FspI TGC'GCA
Cuts at: 4048 4048
Size: 5542
HaeII r_GCGC'y
Cuts at: 1678 2017 2602 4689 1678
Size: 339 585 2087 2531
Fragments arranged by size:
2531 2087 585 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 4183 4215 4257
Size: 32 135 32 710 32 1869 32 42
Cuts at: 4257 4289 1373
Size: 32 2626
Fragments arranged by size:
2626 1869 710 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2886 3132 669
Size: 988 281 948 246 3079
Fragments arranged by size:
3079 988 948 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5542
HpaI GTT'AAC
Cuts at: 3132 3132
Size: 5542
KpnI G_GTAC'C
Cuts at: 2340 2867 2340
Size: 527 5015
MfeI C'AATT_G
Cuts at: 2409 3141 2409
Size: 732 4810
MluI A'CGCG_T
Cuts at: 5418 5418
Size: 5542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 4656 4840 1305
Size: 1005 2346 184 2007
Fragments arranged by size:
2346 2007 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3720
Fragments arranged by size:
3720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2302 3500 3859 4018 518
Size: 648 1021 115 1198 359 159 2042
Fragments arranged by size:
2042 1198 1021 648 359 159 115
MspA1I CmG'CkG
Cuts at: 1606 2263 2367 2397 2587 2691 2958 3635 4783
Size: 657 104 30 190 104 267 677 1148
Cuts at: 4783 5028 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 657 267 245 190 104
104 30
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4990
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5542
NotI GC'GGCC_GC
Cuts at: 2989 2989
Size: 5542
NruI TCG'CGA
Cuts at: 2613 2613
Size: 5542
NspI r_CATG'y
Cuts at: 1132 1229 2480 2900 4445 1132
Size: 97 1251 420 1545 2229
Fragments arranged by size:
2229 1545 1251 420 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5542
PciI A'CATG_T
Cuts at: 4441 4441
Size: 5542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5542
PmeI GTTT'AAAC
Cuts at: 2846 2846
Size: 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2125 3611 3643 5124 5156 2093
Size: 32 1486 32 1481 32 2479
Fragments arranged by size:
2479 1486 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5542
PsiI TTA'TAA
Cuts at: 3112 3112
Size: 5542
PstI C_TGCA'G
Cuts at: 830 2579 2894 830
Size: 1749 315 3478
Fragments arranged by size:
3478 1749 315
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5542
PvuII CAG'CTG
Cuts at: 2263 2367 2587 2263
Size: 104 220 5218
Fragments arranged by size:
5218 220 104
SalI G'TCGA_C
Cuts at: 1936 2884 1936
Size: 948 4594
SbfI CC_TGCA'GG
Cuts at: 2579 2894 2579
Size: 315 5227
ScaI AGT'ACT
Cuts at: 2294 3790 5502 2294
Size: 1496 1712 2334
Fragments arranged by size:
2334 1712 1496
SfcI C'TryA_G
Cuts at: 826 1045 2575 2890 4025 4706 4897 826
Size: 219 1530 315 1135 681 191 1471
Fragments arranged by size:
1530 1471 1135 681 315 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5542
SmaI CCC'GGG
Cuts at: 1872 2869 1872
Size: 997 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3646 4547 4809 820
Size: 197 926 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3333 493
Size: 2840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5542
SphI G_CATG'C
Cuts at: 1132 2900 1132
Size: 1768 3774
SspI AAT'ATT
Cuts at: 5 52 3466 5
Size: 47 3414 2081
Fragments arranged by size:
3414 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5542
StyI C'CwwG_G
Cuts at: 513 1065 2250 2981 513
Size: 552 1185 731 3074
Fragments arranged by size:
3074 1185 731 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2661 3569 3586 3739 3924 4369 325
Size: 1255 1081 908 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1081 908 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2270 2292
Size: 274 80 33 51 1443 81 212 22
Cuts at: 2292 2352 3788 5500 96
Size: 60 1436 1712 138
Fragments arranged by size:
1712 1443 1436 274 212 138 81 80
60 51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2717 2749 5502 5534 2717
Size: 32 2753 32 2725
Fragments arranged by size:
2753 2725 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5542
XbaI T'CTAG_A
Cuts at: 2878 2878
Size: 5542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2408 2408
Size: 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5542
XmaI C'CCGG_G
Cuts at: 1870 2867 1870
Size: 997 4545
XmnI GAAnn'nnTTC
Cuts at: 2639 3671 2639
Size: 1032 4510
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 276
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI