Restriction map for
pFN21AB6529
(Circular) (Six-base) MAPSORT of: pFN21AB6529.seq Check: 1412 from: 1 to: 5800
pFN21AB6529 5800 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2046 3610 278
Size: 53 83 186 936 510 1564 2468
Fragments arranged by size:
2468 1564 936 510 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 2565 3121 2565
Size: 556 5244
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2477 2516 3034 3863 5247 5515 1632
Size: 620 225 39 518 829 1384 268 1917
Fragments arranged by size:
1917 1384 829 620 518 268 225 39
AflII C'TTAA_G
Cuts at: 820 1017 2417 820
Size: 197 1400 4203
Fragments arranged by size:
4203 1400 197
AflIII A'CryG_T
Cuts at: 1203 2079 4699 5676 1203
Size: 876 2620 977 1327
Fragments arranged by size:
2620 1327 977 876
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 2140 2623 4529 2140
Size: 483 1906 3411
Fragments arranged by size:
3411 1906 483
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2392 2710 2752 2788 3543 5115 2392
Size: 318 42 36 755 1572 3077
Fragments arranged by size:
3077 1572 755 318 42 36
ApaLI G'TGCA_C
Cuts at: 2444 3857 5013 2444
Size: 1413 1156 3231
Fragments arranged by size:
3231 1413 1156
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2576 3125 1870
Size: 73 633 549 4545
Fragments arranged by size:
4545 633 549 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2565 2725 3078 3121 4576
Size: 325 217 627 778 160 353 43 1455
Cuts at: 4576 618
Size: 1842
Fragments arranged by size:
1842 1455 778 627 353 325 217 160
43
BanII G_rGCy'C
Cuts at: 1391 2265 2575 3119 1391
Size: 874 310 544 4072
Fragments arranged by size:
4072 874 544 310
BbeI G_GCGC'C
Cuts at: 3082 3082
Size: 5800
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 928
Size: 971 367 4462
Fragments arranged by size:
4462 971 367
BbvCI CC'TCA_GC
Cuts at: 2588 2588
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2906 3087 4411
Size: 107 122 71 631 605 1234 181 1324
Cuts at: 4411 136
Size: 1525
Fragments arranged by size:
1525 1324 1234 631 605 181 122 107
71
BglII A'GATC_T
Cuts at: 2193 5795 2193
Size: 3602 2198
BlpI GC'TnA_GC
Cuts at: 2171 3217 2171
Size: 1046 4754
Bme1580I G_kGCm'C
Cuts at: 1790 2448 3861 5017 1790
Size: 658 1413 1156 2573
Fragments arranged by size:
2573 1413 1156 658
BmgBI CAC'GTC
Cuts at: 1318 2095 2881 1318
Size: 777 786 4237
Fragments arranged by size:
4237 786 777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2879 2891 4460 1239
Size: 1640 12 1569 2579
Fragments arranged by size:
2579 1640 1569 12
Bpu10I CC'TnA_GC
Cuts at: 2588 2588
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2080 2551 3591 493
Size: 711 876 471 1040 2702
Fragments arranged by size:
2702 1040 876 711 471
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2532 3079 3607
Size: 53 83 186 936 510 489 547 528
Cuts at: 3607 3989 4624 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 547 528 510 489 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2016 4233 4631 4905 5052 5463 2016
Size: 2217 398 274 147 411 2353
Fragments arranged by size:
2353 2217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2278 2390 1186
Size: 1092 112 4596
Fragments arranged by size:
4596 1092 112
BseYI C'CCAG_C
Cuts at: 2281 2435 2964 5003 5499 2281
Size: 154 529 2039 496 2582
Fragments arranged by size:
2582 2039 529 496 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2765 1163
Size: 573 1029 4198
Fragments arranged by size:
4198 1029 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2901 3250 4011 5039 1540
Size: 275 186 900 349 761 1028 2301
Fragments arranged by size:
2301 1028 900 761 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2448 3861 3946 5017 2126
Size: 322 1413 85 1071 2909
Fragments arranged by size:
2909 1413 1071 322 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
BsmBI CGTCTCn'nnnn_
Cuts at: 2948 2948
Size: 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2265 2448 2575 3119 3861 3946
Size: 399 336 139 183 127 544 742 85
Cuts at: 3946 5017 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 544 399 336 183 139
127 85
BspEI T'CCGG_A
Cuts at: 2016 5463 2016
Size: 3447 2353
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2862 3155 844
Size: 2018 293 3489
Fragments arranged by size:
3489 2018 293
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3057 4295 4469 65
Size: 1183 1809 1238 174 1396
Fragments arranged by size:
1809 1396 1238 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 2206 1925
Size: 281 5519
BssSI C'ACGA_G
Cuts at: 2993 3860 4872 2993
Size: 867 1012 3921
Fragments arranged by size:
3921 1012 867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 2554 1184
Size: 1370 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 2435 2435
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2193 2341 2382 2706 3130 3497 3882 3899
Size: 221 148 41 324 424 367 385 17
Cuts at: 3899 5351 5437 5449 5795 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 424 385 367 346 324 221
148 86 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2946 2946
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2828 513
Size: 552 1763 3485
Fragments arranged by size:
3485 1763 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2080 4807 809
Size: 507 764 2727 1802
Fragments arranged by size:
2727 1802 764 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2157 2766
Size: 54 1425 50 275 18 297 30 609
Cuts at: 2766 2898 3247 4136 5765 8
Size: 132 349 889 1629 43
Fragments arranged by size:
1629 1425 889 609 349 297 275 132
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2898 3247 1537
Size: 275 1086 349 4090
Fragments arranged by size:
4090 1086 349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 3730 1353
Size: 1593 784 3423
Fragments arranged by size:
3423 1593 784
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2477 2516 2879 2891 3034 3863
Size: 393 620 225 39 363 12 143 829
Cuts at: 3863 4460 5247 5515 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 829 787 620 597 393 363 268
225 143 39 12
EcoO109I rG'GnC_Cy
Cuts at: 2106 2452 2908 2941 2106
Size: 346 456 33 4965
Fragments arranged by size:
4965 456 346 33
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 2297 4306 2297
Size: 2009 3791
HaeII r_GCGC'y
Cuts at: 1678 2893 3082 4947 1678
Size: 1215 189 1865 2531
Fragments arranged by size:
2531 1865 1215 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2609 2641 4441
Size: 32 135 32 554 32 451 32 1800
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1800 554 451 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2047 3144 3390 669
Size: 988 281 109 1097 246 3079
Fragments arranged by size:
3079 1097 988 281 246 109
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 3078 3078
Size: 5800
KpnI G_GTAC'C
Cuts at: 2569 3125 2569
Size: 556 5244
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2159 10
Size: 54 1425 343 327 3651
Fragments arranged by size:
3651 1425 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 3758 4117 4276 518
Size: 648 1194 1398 359 159 2042
Fragments arranged by size:
2042 1398 1194 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2562 2592 2698 2821 3216 3893 5041
Size: 497 459 30 106 123 395 677 1148
Cuts at: 5041 5286 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 497 459 395 245 123
106 30
NarI GG'CG_CC
Cuts at: 3079 3079
Size: 5800
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2242 2528 3158 4703 1132
Size: 97 1013 286 630 1545 2229
Fragments arranged by size:
2229 1545 1013 630 286 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 2614 1108
Size: 1284 222 4294
Fragments arranged by size:
4294 1284 222
PfoI T'CCnGG_A
Cuts at: 2265 2937 2265
Size: 672 5128
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2941 2452
Size: 489 5311
PshAI GACnn'nnGTC
Cuts at: 1613 2093 1613
Size: 480 5320
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2349 3152 830
Size: 1519 803 3478
Fragments arranged by size:
3478 1519 803
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2592 2698 2821 2592
Size: 106 123 5571
Fragments arranged by size:
5571 123 106
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SanDI GG'GwC_CC
Cuts at: 2941 2941
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SexAI A'CCwGG_T
Cuts at: 2316 2391 2316
Size: 75 5725
SfcI C'TryA_G
Cuts at: 826 1045 2345 3148 4283 4964 5155 826
Size: 219 1300 803 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 803 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2906 2906
Size: 5800
SfoI GGC'GCC
Cuts at: 3080 3080
Size: 5800
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2417 3904 4805 5067 820
Size: 197 926 474 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 474 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2415 1517
Size: 898 4902
StyI C'CwwG_G
Cuts at: 513 1065 3239 513
Size: 552 2174 3074
Fragments arranged by size:
3074 2174 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 3827 3844 3997 4182 4627 325
Size: 1255 1350 897 17 153 185 445 1498
Fragments arranged by size:
1498 1350 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 4046 5758
Size: 274 80 33 51 1443 444 1625 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1625 1443 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 2810 3929 2810
Size: 1119 4681
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2044 3608 276
Size: 53 83 186 936 510 1564 2468
Fragments arranged by size:
2468 1564 936 510 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SrfI
SwaI XcmI