Restriction map for
pFN21AB6528
(Circular) (Six-base) MAPSORT of: pFN21AB6528.seq Check: 1752 from: 1 to: 5869
pFN21AB6528 5869 bp
With 182 enzymes: SgfI *
September 11, 2009 17:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2436 2436
Size: 5869
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5316 5584 1632
Size: 2300 1384 268 1917
Fragments arranged by size:
2300 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 2477 2518 4768 5745 1203
Size: 1274 41 2250 977 1327
Fragments arranged by size:
2250 1327 1274 977 41
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 2519 1166
Size: 1353 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3165 1184
Size: 1981 3888
AlwNI CAG_nnn'CTG
Cuts at: 2347 3612 5184 2347
Size: 1265 1572 3032
Fragments arranged by size:
3032 1572 1265
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 2594 2990 3178 3404 3556 5665 1493
Size: 1101 396 188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 396 226 188 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 2354 3194 1870
Size: 73 411 840 4545
Fragments arranged by size:
4545 840 411 73
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 2496 3190 4645 618
Size: 325 217 627 630 79 694 1455 1842
Fragments arranged by size:
1842 1455 694 630 627 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2094 3188 1391
Size: 703 1094 4072
Fragments arranged by size:
4072 1094 703
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2941 2975 4060 4094 1903
Size: 34 1004 34 1085 34 3678
Fragments arranged by size:
3678 1085 1004 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3761 4971 2598
Size: 1163 1210 3496
Fragments arranged by size:
3496 1210 1163
BclI T'GATC_A
Cuts at: 1548 3012 1548
Size: 1464 4405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 4480 136
Size: 107 122 71 631 605 355 2453 1525
Fragments arranged by size:
2453 1525 631 605 355 122 107 71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 3286 3286
Size: 5869
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2501 3930 5086 1790
Size: 564 147 1429 1156 2573
Fragments arranged by size:
2573 1429 1156 564 147
BmgBI CAC'GTC
Cuts at: 1318 2478 1318
Size: 1160 4709
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2788 3600 4558
Size: 416 53 83 399 24 1371 812 958
Cuts at: 4558 442
Size: 1753
Fragments arranged by size:
1753 1371 958 812 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4529 1239
Size: 3290 2579
Bpu10I CC'TnA_GC
Cuts at: 2283 2283
Size: 5869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 3994 4859 5157 2630
Size: 1364 865 298 3342
Fragments arranged by size:
3342 1364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2901 4532 882
Size: 617 1402 1631 2219
Fragments arranged by size:
2219 1631 1402 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3660 493
Size: 711 1317 1139 2702
Fragments arranged by size:
2702 1317 1139 711
BsaBI GATnn'nnATC
Cuts at: 3017 5863 3017
Size: 2846 3023
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3676 4058 4693 275
Size: 53 83 186 936 2143 382 635 1451
Fragments arranged by size:
2143 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2247 4302 4700 4974 5121 5532 2247
Size: 2055 398 274 147 411 2584
Fragments arranged by size:
2584 2055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5644
Fragments arranged by size:
5644 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5869
BseYI C'CCAG_C
Cuts at: 5072 5568 5072
Size: 496 5373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3054 3319 4080 5108 1540
Size: 275 186 1053 265 761 1028 2301
Fragments arranged by size:
2301 1053 1028 761 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2862 3930 4015 5086 2070
Size: 792 1068 85 1071 2853
Fragments arranged by size:
2853 1071 1068 792 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2094 2354 2501 2862 3188 3930
Size: 399 280 24 260 147 361 326 742
Cuts at: 3930 4015 5086 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 326 280 260
147 85 24
BspEI T'CCGG_A
Cuts at: 2247 5532 2247
Size: 3285 2584
BspHI T'CATG_A
Cuts at: 2094 2307 3758 5488 2094
Size: 213 1451 1730 2475
Fragments arranged by size:
2475 1730 1451 213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2436 2539 3224 844
Size: 1592 103 685 3489
Fragments arranged by size:
3489 1592 685 103
BsrBI CCG'CTC
Cuts at: 1394 1823 3756 1394
Size: 429 1933 3507
Fragments arranged by size:
3507 1933 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4364 4538 65
Size: 1183 3116 174 1396
Fragments arranged by size:
3116 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2405 2414 4513 4700 1700
Size: 110 595 9 2099 187 2869
Fragments arranged by size:
2869 2099 595 187 110 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5869
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 2250 2674 3199 3566 3951 3968 5420 5506
Size: 278 424 525 367 385 17 1452 86
Cuts at: 5506 5518 5864 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 525 424 385 367 346 278
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2745 513
Size: 552 939 177 564 3637
Fragments arranged by size:
3637 939 564 552 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5130
Fragments arranged by size:
5130 683 56
BtsI GCAGTG_nn'
Cuts at: 3035 3385 4179 4199 3035
Size: 350 794 20 4705
Fragments arranged by size:
4705 794 350 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 2541 3173 2541
Size: 632 5237
DraIII CAC_nnn'GTG
Cuts at: 2379 2379
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2183 3071 4876 809
Size: 507 867 888 1805 1802
Fragments arranged by size:
1805 1802 888 867 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2742 3316 4205
Size: 54 1425 50 275 18 912 574 889
Cuts at: 4205 5834 8
Size: 1629 43
Fragments arranged by size:
1629 1425 912 889 574 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 3799 1353
Size: 953 1493 3423
Fragments arranged by size:
3423 1493 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 2822 4456 4830 4976 387
Size: 1064 138 690 543 1634 374 146 1280
Fragments arranged by size:
1634 1280 1064 690 543 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3932 4529 5316 5584 1239
Size: 393 2300 597 787 268 1524
Fragments arranged by size:
2300 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2344 2344
Size: 5869
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 5016 1678
Size: 3338 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2266 2267 2298 4510
Size: 32 135 32 663 31 1 31 2212
Cuts at: 4510 4542 4584 4616 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2212 663 135 42 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2776 3213 3459 669
Size: 988 281 838 437 246 3079
Fragments arranged by size:
3079 988 838 437 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4047
Fragments arranged by size:
4047 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 2744 2969 3827 4186 4345 518
Size: 648 1353 225 225 858 359 159 2042
Fragments arranged by size:
2042 1353 858 648 359 225 225 159
MspA1I CmG'CkG
Cuts at: 1606 2397 3285 3962 5110 5355 1606
Size: 791 888 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 791 677 245
NaeI GCC'GGC
Cuts at: 2407 2416 2407
Size: 9 5860
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4378
Fragments arranged by size:
4378 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NgoMIV G'CCGG_C
Cuts at: 2405 2414 2405
Size: 9 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 3227 4772 1132
Size: 97 1998 1545 2229
Fragments arranged by size:
2229 1998 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 1108
Size: 1632 4237
PfoI T'CCnGG_A
Cuts at: 2733 2733
Size: 5869
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PmlI CAC'GTG
Cuts at: 2521 2521
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2204 1613
Size: 591 5278
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PstI C_TGCA'G
Cuts at: 830 2551 3221 830
Size: 1721 670 3478
Fragments arranged by size:
3478 1721 670
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 2397 2397
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 2900 4117 5829 2900
Size: 1217 1712 2940
Fragments arranged by size:
2940 1712 1217
SexAI A'CCwGG_T
Cuts at: 3165 3165
Size: 5869
SfcI C'TryA_G
Cuts at: 826 1045 2547 2902 3217 4352 5033 5224 826
Size: 219 1502 355 315 1135 681 191 1471
Fragments arranged by size:
1502 1471 1135 681 355 315 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2645 3973 4874 5136 820
Size: 197 926 702 1328 901 262 1553
Fragments arranged by size:
1553 1328 926 901 702 262 197
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 2945 1517
Size: 1428 4441
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 3308 513
Size: 552 939 198 1106 3074
Fragments arranged by size:
3074 1106 939 552 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2131 2435 3896 3913 4066 4251 4696
Size: 1255 551 304 1461 17 153 185 445
Cuts at: 4696 325
Size: 1498
Fragments arranged by size:
1498 1461 1255 551 445 304 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2399 2898 4115
Size: 274 80 33 51 1443 422 499 1217
Cuts at: 4115 5827 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1217 499 422 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 3998 3998
Size: 5869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI