Restriction map for
pFN21AB6526
(Circular) (Six-base) MAPSORT of: pFN21AB6526.seq Check: 5277 from: 1 to: 5782
pFN21AB6526 5782 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 3845 5229 5497 1632
Size: 783 1430 1384 268 1917
Fragments arranged by size:
1917 1430 1384 783 268
AflII C'TTAA_G
Cuts at: 820 1017 2873 820
Size: 197 1856 3729
Fragments arranged by size:
3729 1856 197
AflIII A'CryG_T
Cuts at: 1203 2165 4681 5658 1203
Size: 962 2516 977 1327
Fragments arranged by size:
2516 1327 977 962
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2242 2393 2534 3525 5097 2242
Size: 151 141 991 1572 2927
Fragments arranged by size:
2927 1572 991 151 141
ApaI G_GGCC'C
Cuts at: 2283 2283
Size: 5782
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2765 3091 3317 3469 5578 1493
Size: 1272 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 1272 326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
AvrII C'CTAG_G
Cuts at: 2998 2998
Size: 5782
BamHI G'GATC_C
Cuts at: 2943 3112 3479 2943
Size: 169 367 5246
Fragments arranged by size:
5246 367 169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3103 4558 618
Size: 325 217 627 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 3101 1391
Size: 892 818 4072
Fragments arranged by size:
4072 892 818
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2030 928
Size: 971 131 4680
Fragments arranged by size:
4680 971 131
BbvCI CC'TCA_GC
Cuts at: 2542 2542
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2224 5777 2224
Size: 3553 2229
BlpI GC'TnA_GC
Cuts at: 2577 3199 2577
Size: 622 5160
Bme1580I G_kGCm'C
Cuts at: 1790 2283 3843 4999 1790
Size: 493 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 493
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 4442 1239
Size: 1337 1866 2579
Fragments arranged by size:
2579 1866 1337
Bpu10I CC'TnA_GC
Cuts at: 2542 2542
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2997 4445 882
Size: 617 1498 1448 2219
Fragments arranged by size:
2219 1498 1448 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 2521 4215 4613 4887 5034 5445 2499
Size: 22 1694 398 274 147 411 2836
Fragments arranged by size:
2836 1694 411 398 274 147 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5557
Fragments arranged by size:
5557 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 1186
Size: 1288 4494
BseYI C'CCAG_C
Cuts at: 2254 2938 4985 5481 2254
Size: 684 2047 496 2555
Fragments arranged by size:
2555 2047 684 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2294 2647 1163
Size: 573 519 39 353 4298
Fragments arranged by size:
4298 573 519 353 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 3101 3843 3928 4999 1391
Size: 399 493 818 742 85 1071 2174
Fragments arranged by size:
2174 1071 818 742 493 399 85
BspEI T'CCGG_A
Cuts at: 2499 5445 2499
Size: 2946 2836
BspHI T'CATG_A
Cuts at: 3067 3671 5401 3067
Size: 604 1730 3448
Fragments arranged by size:
3448 1730 604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2370 3137 844
Size: 1526 767 3489
Fragments arranged by size:
3489 1526 767
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 4334
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 2353 1184
Size: 1169 4613
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2224 2943 3112 3479 3864 3881 5333 5419
Size: 252 719 169 367 385 17 1452 86
Cuts at: 5419 5431 5777 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 719 385 367 346 252 169
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2274 2274
Size: 5782
BtgI C'CryG_G
Cuts at: 513 1065 2623 513
Size: 552 1558 3672
Fragments arranged by size:
3672 1558 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3033 487
Size: 683 56 1807 3236
Fragments arranged by size:
3236 1807 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 3298 4092 4112 2378
Size: 920 794 20 4048
Fragments arranged by size:
4048 920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 2137 3086 2137
Size: 949 4833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2111 4789 809
Size: 507 795 2678 1802
Fragments arranged by size:
2678 1802 795 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 3229 4118
Size: 54 1425 50 275 18 352 1047 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1047 889 352 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2429 3712 1353
Size: 1076 1283 3423
Fragments arranged by size:
3423 1283 1076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2546 4369 4743 4889 387
Size: 1064 138 957 1823 374 146 1280
Fragments arranged by size:
1823 1280 1064 957 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2576 3845 4442 5229 5497 1239
Size: 393 783 161 1269 597 787 268 1524
Fragments arranged by size:
1524 1269 787 783 597 393 268 161
EcoO109I rG'GnC_Cy
Cuts at: 2279 2280 2390 2531 2851 2279
Size: 1 110 141 320 5210
Fragments arranged by size:
5210 320 141 110 1
EcoRI G'AATT_C
Cuts at: 2765 3091 3469 2765
Size: 326 378 5078
Fragments arranged by size:
5078 378 326
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2619 2651 4423 4455 4497
Size: 32 135 32 1047 32 1772 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1772 1047 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2163 2701 2971 3126 3372 669
Size: 988 281 225 538 270 155 246 3079
Fragments arranged by size:
3079 988 538 281 270 246 225 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4099 4258 518
Size: 648 2574 359 159 2042
Fragments arranged by size:
2574 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2258 2838 3198 3875 5023 5268 1606
Size: 652 580 360 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 580 360 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2169 3140 4685 1132
Size: 97 940 971 1545 2229
Fragments arranged by size:
2229 1545 971 940 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 2165 4681 2165
Size: 2516 3266
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2390 2390
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PspOMI G'GGCC_C
Cuts at: 2279 2279
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2349 2385 3134 830
Size: 1519 36 749 3478
Fragments arranged by size:
3478 1519 749 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2258 2838 2258
Size: 580 5202
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 2132 4030 5742 2132
Size: 1898 1712 2172
Fragments arranged by size:
2172 1898 1712
SfcI C'TryA_G
Cuts at: 826 1045 2294 2345 2381 2778 3130 4265 4946
Size: 219 1249 51 36 397 352 1135 681
Cuts at: 4946 5137 826
Size: 191 1471
Fragments arranged by size:
1471 1249 1135 681 397 352 219 191
51 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2873 3886 4787 5049 820
Size: 197 926 930 1013 901 262 1553
Fragments arranged by size:
1553 1013 930 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 4702
StyI C'CwwG_G
Cuts at: 513 1065 2010 2998 3039 3221 513
Size: 552 945 988 41 182 3074
Fragments arranged by size:
3074 988 945 552 182 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2506 2739
Size: 274 80 33 51 1443 153 376 233
Cuts at: 2739 4028 5740 96
Size: 1289 1712 138
Fragments arranged by size:
1712 1443 1289 376 274 233 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2438 3911 2438
Size: 1473 4309
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI