Restriction map for
pFN21AB6524
(Circular) (Six-base) MAPSORT of: pFN21AB6524.seq Check: 3143 from: 1 to: 5866
pFN21AB6524 5866 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2470 2470
Size: 5866
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2592 3209 1937
Size: 655 617 4594
Fragments arranged by size:
4594 655 617
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 2654 2984 3929 5313 5581 1632
Size: 866 156 330 945 1384 268 1917
Fragments arranged by size:
1917 1384 945 866 330 268 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 3105 1166
Size: 1939 3927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 2362 3609 5181 2362
Size: 1247 1572 3047
Fragments arranged by size:
3047 1572 1247
ApaLI G'TGCA_C
Cuts at: 2114 3098 3923 5079 2114
Size: 984 825 1156 2901
Fragments arranged by size:
2901 1156 984 825
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 2805 3196 3563 2805
Size: 391 367 5108
Fragments arranged by size:
5108 391 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2713 2785 2890 3061 3142
Size: 325 217 627 926 72 105 171 81
Cuts at: 3142 3187 4642 618
Size: 45 1455 1842
Fragments arranged by size:
1842 1455 926 627 325 217 171 105
81 72 45
BanII G_rGCy'C
Cuts at: 1391 2304 2505 3185 1391
Size: 913 201 680 4072
Fragments arranged by size:
4072 913 680 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2227 4477 136
Size: 107 122 71 631 605 555 2250 1525
Fragments arranged by size:
2250 1525 631 605 555 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2674 2790 2895 3066 3102 3147 3927
Size: 328 556 116 105 171 36 45 780
Cuts at: 3927 5083 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 780 556 328 171 116 105
45 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2288 2390 2831 4526 1239
Size: 947 102 102 441 1695 2579
Fragments arranged by size:
2579 1695 947 441 102 102
Bpu10I CC'TnA_GC
Cuts at: 2151 2151
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2964 4529 882
Size: 617 1465 1565 2219
Fragments arranged by size:
2219 1565 1465 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4299 4697 4971 5118 5529 4299
Size: 398 274 147 411 4636
Fragments arranged by size:
4636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2019 2024 2456 3044 1186
Size: 833 5 432 588 4008
Fragments arranged by size:
4008 833 588 432 5
BseYI C'CCAG_C
Cuts at: 2056 2073 2241 5069 5565 2056
Size: 17 168 2828 496 2357
Fragments arranged by size:
2828 2357 496 168 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2992 1163
Size: 573 1256 4037
Fragments arranged by size:
4037 1256 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3102 3927 4012 5083 2118
Size: 984 825 85 1071 2901
Fragments arranged by size:
2901 1071 984 825 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
BsmBI CGTCTCn'nnnn_
Cuts at: 2126 2126
Size: 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2304 2505 2674 2790 2895 3066
Size: 399 328 186 201 169 116 105 171
Cuts at: 3066 3102 3147 3185 3927 4012 5083 1391
Size: 36 45 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 201 186 171
169 116 105 85 45 38 36
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 2470 3221 844
Size: 1382 244 751 3489
Fragments arranged by size:
3489 1382 751 244
BsrBI CCG'CTC
Cuts at: 1394 1823 3112 3753 1394
Size: 429 1289 641 3507
Fragments arranged by size:
3507 1289 641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2746 4361 4535 65
Size: 1183 1498 1615 174 1396
Fragments arranged by size:
1615 1498 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2946 4510 4697 1700
Size: 110 1136 1564 187 2869
Fragments arranged by size:
2869 1564 1136 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 2036 3926 4938 2036
Size: 1890 1012 2964
Fragments arranged by size:
2964 1890 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 2878 1184
Size: 1694 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 2261 2261
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2805 3196 3563 3948 3965 5417 5503 5515
Size: 833 391 367 385 17 1452 86 12
Cuts at: 5515 5861 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 833 391 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2593 2593
Size: 5866
BtgI C'CryG_G
Cuts at: 513 1065 2004 2545 3016 513
Size: 552 939 541 471 3363
Fragments arranged by size:
3363 939 552 541 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3382 4176 4196 3004
Size: 378 794 20 4674
Fragments arranged by size:
4674 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3048 3068 3313
Size: 54 1425 50 275 18 1218 20 245
Cuts at: 3313 4202 5831 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1218 889 275 245 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2960 3796 1353
Size: 671 936 836 3423
Fragments arranged by size:
3423 936 836 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2288 2390 2498 2654 2831 2984
Size: 393 554 102 102 108 156 177 153
Cuts at: 2984 3929 4526 5313 5581 1239
Size: 945 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 554 393 268 177
156 153 108 102 102
EcoO109I rG'GnC_Cy
Cuts at: 2380 2390 2845 2380
Size: 10 455 5401
Fragments arranged by size:
5401 455 10
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FspI TGC'GCA
Cuts at: 2426 4372 2426
Size: 1946 3920
HaeII r_GCGC'y
Cuts at: 1678 3138 5013 1678
Size: 1460 1875 2531
Fragments arranged by size:
2531 1875 1460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373
Size: 32 135 32 2935 32 42 32 2626
Fragments arranged by size:
2935 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2731 3210 3456 669
Size: 988 281 793 479 246 3079
Fragments arranged by size:
3079 988 793 479 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 4980 5164 1305
Size: 1704 1971 184 2007
Fragments arranged by size:
2007 1971 1704 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 2993 3105 3824 4183 4342 518
Size: 648 1434 393 112 719 359 159 2042
Fragments arranged by size:
2042 1434 719 648 393 359 159 112
MspA1I CmG'CkG
Cuts at: 1606 2174 2518 2667 3282 3959 5107 5352 1606
Size: 568 344 149 615 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 615 568 344 245 149
NaeI GCC'GGC
Cuts at: 2948 2948
Size: 5866
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NgoMIV G'CCGG_C
Cuts at: 2946 2946
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2159 3224 4769 1132
Size: 97 930 1065 1545 2229
Fragments arranged by size:
2229 1545 1065 930 97
PasI CC'CwG_GG
Cuts at: 1348 2674 1348
Size: 1326 4540
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2801 1108
Size: 1693 4173
PfoI T'CCnGG_A
Cuts at: 2808 2808
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2380
Size: 5866
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 2779 3218 830
Size: 1949 439 3478
Fragments arranged by size:
3478 1949 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2518 2518
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SanDI GG'GwC_CC
Cuts at: 2380 2380
Size: 5866
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2775 3214 4349 5030 5221 826
Size: 219 1730 439 1135 681 191 1471
Fragments arranged by size:
1730 1471 1135 681 439 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3970 4871 5133 820
Size: 197 926 2027 901 262 1553
Fragments arranged by size:
2027 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2484 2538 2709 3305 513
Size: 552 939 117 363 54 171 596 3074
Fragments arranged by size:
3074 939 596 552 363 171 117 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 2226 2255 2851 3893 3910 4063
Size: 1255 526 120 29 596 1042 17 153
Cuts at: 4063 4248 4693 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1042 596 526 445 185 153
120 29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4112 5824 96
Size: 274 80 33 51 1443 2135 1712 138
Fragments arranged by size:
2135 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 2706 2738 5826 5858 2160
Size: 32 514 32 3088 32 2168
Fragments arranged by size:
3088 2168 514 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI