Restriction map for
pFN21AB6517
(Circular) (Six-base) MAPSORT of: pFN21AB6517.seq Check: 5677 from: 1 to: 5791
pFN21AB6517 5791 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2065 3134 1937
Size: 128 1069 4594
Fragments arranged by size:
4594 1069 128
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3049 3854 5238 5506 1632
Size: 1417 805 1384 268 1917
Fragments arranged by size:
1917 1417 1384 805 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 2811 4520 2811
Size: 1709 4082
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 3534 5106 3534
Size: 1572 4219
ApaLI G'TGCA_C
Cuts at: 2019 3848 5004 2019
Size: 1829 1156 2806
Fragments arranged by size:
2806 1829 1156
ApoI r'AATT_y
Cuts at: 1493 2752 3012 3055 3100 3326 3478 5587 1493
Size: 1259 260 43 45 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 260 226 152 45 43
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 1870
Size: 73 1173 4545
Fragments arranged by size:
4545 1173 73
BamHI G'GATC_C
Cuts at: 2591 3121 3488 2591
Size: 530 367 4894
Fragments arranged by size:
4894 530 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3112 4567 618
Size: 325 217 627 1325 1455 1842
Fragments arranged by size:
1842 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3110 1391
Size: 1719 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 2207 2612 928
Size: 971 137 171 405 4107
Fragments arranged by size:
4107 971 405 171 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3982 4016 1903
Size: 34 50 34 1961 34 3678
Fragments arranged by size:
3678 1961 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2597 2974 5786 2597
Size: 377 2812 2602
Fragments arranged by size:
2812 2602 377
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2337 3852 5008 1790
Size: 233 314 1515 1156 2573
Fragments arranged by size:
2573 1515 1156 314 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 4480 442
Size: 416 53 83 399 24 2105 958 1753
Fragments arranged by size:
2105 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2262 2817 4451 1239
Size: 1023 555 1634 2579
Fragments arranged by size:
2579 1634 1023 555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2791 3014 3916 4781 5079 2791
Size: 223 902 865 298 3503
Fragments arranged by size:
3503 902 865 298 223
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4454 882
Size: 617 2955 2219
Fragments arranged by size:
2955 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 2596 5785 2596
Size: 3189 2602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3598 3980 4615 275
Size: 53 83 186 936 2065 382 635 1451
Fragments arranged by size:
2065 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 1178
Size: 30 165 30 759 30 4777
Fragments arranged by size:
4777 759 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5791
BseYI C'CCAG_C
Cuts at: 4994 5490 4994
Size: 496 5295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2287 2425 2485 1163
Size: 573 551 138 60 4469
Fragments arranged by size:
4469 573 551 138 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 3852 3937 5008 2023
Size: 1829 85 1071 2806
Fragments arranged by size:
2806 1829 1071 85
BsmI GAATG_Cn'
Cuts at: 2558 3302 3395 2558
Size: 744 93 4954
Fragments arranged by size:
4954 744 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2337 3110 3852 3937 5008 1391
Size: 399 233 314 773 742 85 1071 2174
Fragments arranged by size:
2174 1071 773 742 399 314 233 85
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 2245 2713 2802 3680 5410 2245
Size: 468 89 878 1730 2626
Fragments arranged by size:
2626 1730 878 468 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3146 844
Size: 2302 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4435 4622 1700
Size: 110 2625 187 2869
Fragments arranged by size:
2869 2625 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 2833 4595 2833
Size: 1762 4029
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2188
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2591 2597 2974 3121 3488 3873 3890 5342
Size: 619 6 377 147 367 385 17 1452
Cuts at: 5342 5428 5440 5786 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 619 385 377 367 346 147
86 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2094 2129 529
Size: 35 1530 35 4191
Fragments arranged by size:
4191 1530 35 35
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DraIII CAC_nnn'GTG
Cuts at: 2577 2577
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2306 4798 809
Size: 507 990 2492 1802
Fragments arranged by size:
2492 1802 990 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 3238 4127
Size: 54 1425 50 275 18 421 987 889
Cuts at: 4127 5756 8
Size: 1629 43
Fragments arranged by size:
1629 1425 987 889 421 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 3721 1353
Size: 1298 1070 3423
Fragments arranged by size:
3423 1298 1070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 2817 3049 3854 4451 5238 5506
Size: 393 630 555 232 805 597 787 268
Cuts at: 5506 1239
Size: 1524
Fragments arranged by size:
1524 805 787 630 597 555 393 268
232
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3008 3008
Size: 5791
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 4938 1678
Size: 3260 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2576 2608 2797
Size: 32 135 32 590 32 382 32 189
Cuts at: 2797 2829 2959 2991 4432 4464 4506 4538 1373
Size: 32 130 32 1441 32 42 32 2626
Fragments arranged by size:
2626 1441 590 382 189 135 130 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2767 3135 3381 669
Size: 988 281 829 368 246 3079
Fragments arranged by size:
3079 988 829 368 281 246
HindIII A'AGCT_T
Cuts at: 748 3082 748
Size: 2334 3457
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2747 4905 5089 1305
Size: 1442 2158 184 2007
Fragments arranged by size:
2158 2007 1442 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 10
Size: 54 1425 343 421 3548
Fragments arranged by size:
3548 1425 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 3749 4108 4267 518
Size: 648 1081 1502 359 159 2042
Fragments arranged by size:
2042 1502 1081 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3207 3884 5032 5277 1606
Size: 1601 677 1148 245 2120
Fragments arranged by size:
2120 1601 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5239
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2682 3149 4694 1132
Size: 97 1453 467 1545 2229
Fragments arranged by size:
2229 1545 1453 467 97
PasI CC'CwG_GG
Cuts at: 1348 2038 1348
Size: 690 5101
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3085 1108
Size: 1977 3814
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 2211 3139 4274 4955 5146 826
Size: 219 1166 928 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 928 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2770 3029 3895 4796 5058 820
Size: 197 926 827 259 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 827 262 259 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 3041 152
Size: 2889 2902
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 2238 1517
Size: 721 5070
StyI C'CwwG_G
Cuts at: 513 1065 2181 2704 3230 513
Size: 552 1116 523 526 3074
Fragments arranged by size:
3074 1116 552 526 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2641 3818 3835 3988 4173 4618 325
Size: 1255 1061 1177 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1177 1061 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 2668 2996
Size: 274 80 33 51 1443 378 313 328
Cuts at: 2996 4037 5749 96
Size: 1041 1712 138
Fragments arranged by size:
1712 1443 1041 378 328 313 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 2882 1316
Size: 1566 4225
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2602 2646 2602
Size: 44 5747
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI