Restriction map for
pFN21AB6516
(Circular) (Six-base) MAPSORT of: pFN21AB6516.seq Check: 7567 from: 1 to: 5860
pFN21AB6516 5860 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2588 2588
Size: 5860
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 2660 3181 2660
Size: 521 5339
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2677 2683 2701 2748 2863 3923 5307
Size: 756 289 6 18 47 115 1060 1384
Cuts at: 5307 5575 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1060 756 289 268 115 47
18 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 3603 5175 3603
Size: 1572 4288
ApaI G_GGCC'C
Cuts at: 2610 2610
Size: 5860
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3185 1870
Size: 73 1242 4545
Fragments arranged by size:
4545 1242 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2660 3181 4636 618
Size: 325 217 627 873 521 1455 1842
Fragments arranged by size:
1842 1455 873 627 521 325 217
BanII G_rGCy'C
Cuts at: 1391 2400 2610 3179 1391
Size: 1009 210 569 4072
Fragments arranged by size:
4072 1009 569 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 928
Size: 971 383 4506
Fragments arranged by size:
4506 971 383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2433 5855 2433
Size: 3422 2438
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2610 3921 5077 1790
Size: 820 1311 1156 2573
Fragments arranged by size:
2573 1311 1156 820
BmgBI CAC'GTC
Cuts at: 1318 2340 1318
Size: 1022 4838
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 2605 2754
Size: 416 53 83 399 24 975 213 149
Cuts at: 2754 3591 4549 442
Size: 837 958 1753
Fragments arranged by size:
1753 975 958 837 416 399 213 149
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2775 4520 1239
Size: 1052 484 1745 2579
Fragments arranged by size:
2579 1745 1052 484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 2312 2438 5854 2312
Size: 126 3416 2318
Fragments arranged by size:
3416 2318 126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2478 3667 4049 4684
Size: 53 83 186 936 945 1189 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1451 1189 945 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2316 4293 4691 4965 5112 5523 2316
Size: 1977 398 274 147 411 2653
Fragments arranged by size:
2653 1977 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2541 2571 1178
Size: 30 165 30 1138 30 4467
Fragments arranged by size:
4467 1138 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5860
BseYI C'CCAG_C
Cuts at: 2656 5063 5559 2656
Size: 2407 496 2957
Fragments arranged by size:
2957 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 3921 4006 5077 2072
Size: 1849 85 1071 2855
Fragments arranged by size:
2855 1849 1071 85
BsmI GAATG_Cn'
Cuts at: 3074 3371 3464 3074
Size: 297 93 5470
Fragments arranged by size:
5470 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2400 2610 3179 3921 4006 5077
Size: 399 282 328 210 569 742 85 1071
Cuts at: 5077 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 569 399 328 282 210
85
BspEI T'CCGG_A
Cuts at: 2316 5523 2316
Size: 3207 2653
BspHI T'CATG_A
Cuts at: 2850 3749 5479 2850
Size: 899 1730 3231
Fragments arranged by size:
3231 1730 899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2588 3215 844
Size: 1744 627 3489
Fragments arranged by size:
3489 1744 627
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2433 3190 3557 3942 3959 5411 5497 5509
Size: 461 757 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 757 461 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 2545 2664 513
Size: 552 939 50 491 119 3709
Fragments arranged by size:
3709 939 552 491 119 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 3376 4170 4190 2545
Size: 831 794 20 4215
Fragments arranged by size:
4215 831 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DraIII CAC_nnn'GTG
Cuts at: 2711 2711
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2542 2871 3307
Size: 54 1425 50 275 18 712 329 436
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 712 436 329 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3050 3790 1353
Size: 1697 740 3423
Fragments arranged by size:
3423 1697 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 4447 4821 4967 387
Size: 1064 138 681 2177 374 146 1280
Fragments arranged by size:
2177 1280 1064 681 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2388 2677 2683 2701 2748 2775
Size: 393 659 97 289 6 18 47 27
Cuts at: 2775 2863 3923 4520 5307 5575 1239
Size: 88 1060 597 787 268 1524
Fragments arranged by size:
1524 1060 787 659 597 393 289 268
97 88 47 27 18 6
EcoO109I rG'GnC_Cy
Cuts at: 2607 2647 2607
Size: 40 5820
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 5007 1678
Size: 3329 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 4501 4533 4575
Size: 32 135 32 1241 32 1656 32 42
Cuts at: 4575 4607 1373
Size: 32 2626
Fragments arranged by size:
2626 1656 1241 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 2664 3185 2664
Size: 521 5339
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2544 2873 10
Size: 54 1425 343 712 329 2997
Fragments arranged by size:
2997 1425 712 343 329 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2059 3053 3818 4177 4336 518
Size: 648 893 994 765 359 159 2042
Fragments arranged by size:
2042 994 893 765 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2721 3276 3953 5101 5346 1606
Size: 1115 555 677 1148 245 2120
Fragments arranged by size:
2120 1148 1115 677 555 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2054 2545 2664 513
Size: 552 939 50 491 119 3709
Fragments arranged by size:
3709 939 552 491 119 50
NdeI CA'TA_TG
Cuts at: 387 3157 387
Size: 2770 3090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2898 3218 4763 1132
Size: 97 1669 320 1545 2229
Fragments arranged by size:
2229 1669 1545 320 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 2869 1108
Size: 1082 679 4099
Fragments arranged by size:
4099 1082 679
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3036 3430 3036
Size: 394 5466
PspOMI G'GGCC_C
Cuts at: 2606 2606
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2015 3212 830
Size: 1185 1197 3478
Fragments arranged by size:
3478 1197 1185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2721 2721
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2011 2857 3208 4343 5024 5215 826
Size: 219 966 846 351 1135 681 191 1471
Fragments arranged by size:
1471 1135 966 846 681 351 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 2054 2545 2664 3299 513
Size: 552 939 50 491 119 635 3074
Fragments arranged by size:
3074 939 635 552 491 119 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2332 3887 3904 4057 4242 4687
Size: 1255 507 245 1555 17 153 185 445
Cuts at: 4687 325
Size: 1498
Fragments arranged by size:
1555 1498 1255 507 445 245 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2672 4106 5818
Size: 274 80 33 51 1443 695 1434 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1443 1434 695 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2778 2778
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 2093 3989 2093
Size: 1896 3964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI