Restriction map for
pFN21AB6515
(Circular) (Six-base) MAPSORT of: pFN21AB6515.seq Check: 4355 from: 1 to: 5785
pFN21AB6515 5785 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3084 3912 4285 1563
Size: 1521 828 373 3063
Fragments arranged by size:
3063 1521 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2423 2630 3848 5232 5500 1632
Size: 791 207 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 791 268 207
AflII C'TTAA_G
Cuts at: 820 1017 3001 820
Size: 197 1984 3604
Fragments arranged by size:
3604 1984 197
AflIII A'CryG_T
Cuts at: 1203 2447 4684 5661 1203
Size: 1244 2237 977 1327
Fragments arranged by size:
2237 1327 1244 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 2457 1166
Size: 1291 4494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2172 3528 5100 2172
Size: 1356 1572 2857
Fragments arranged by size:
2857 1572 1356
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 3094 3320 3472 5581 1493
Size: 1601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2841 3110 1870
Size: 73 898 269 4545
Fragments arranged by size:
4545 898 269 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 3106 4561 618
Size: 325 217 627 475 844 1455 1842
Fragments arranged by size:
1842 1455 844 627 475 325 217
BanII G_rGCy'C
Cuts at: 1391 2927 3104 1391
Size: 1536 177 4072
Fragments arranged by size:
4072 1536 177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2032 2411 2726 928
Size: 971 133 379 315 3987
Fragments arranged by size:
3987 971 379 315 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3976 4010 1903
Size: 34 50 34 1955 34 3678
Fragments arranged by size:
3678 1955 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 2493 1548
Size: 945 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2695 5780 2695
Size: 3085 2700
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 3846 5002 1790
Size: 2056 1156 2573
Fragments arranged by size:
2573 2056 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2622 3516 4474
Size: 416 53 83 399 24 1205 894 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1205 958 894 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2523 2967 4445 1239
Size: 1136 148 444 1478 2579
Fragments arranged by size:
2579 1478 1136 444 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2092 2122 2914 2944 1178
Size: 30 165 30 689 30 792 30 4019
Fragments arranged by size:
4019 792 689 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2097 2100 2550 2553 2556 2580 2935 1186
Size: 911 3 450 3 3 24 355 4036
Fragments arranged by size:
4036 911 450 355 24 3 3 3
BseYI C'CCAG_C
Cuts at: 4988 5484 4988
Size: 496 5289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
BsmBI CGTCTCn'nnnn_
Cuts at: 2794 2794
Size: 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2927 3104 3846 3931 5002 1391
Size: 399 1137 177 742 85 1071 2174
Fragments arranged by size:
2174 1137 1071 742 399 177 85
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2695 2884 3115 3482 3867 3884 5336 5422
Size: 723 189 231 367 385 17 1452 86
Cuts at: 5422 5434 5780 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 723 385 367 346 231 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2742 487
Size: 683 56 1516 3530
Fragments arranged by size:
3530 1516 683 56
BtsI GCAGTG_nn'
Cuts at: 2531 3301 4095 4115 2531
Size: 770 794 20 4201
Fragments arranged by size:
4201 794 770 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2423 2523 2630 2967 3848 4445
Size: 393 743 48 100 107 337 881 597
Cuts at: 4445 5232 5500 1239
Size: 787 268 1524
Fragments arranged by size:
1524 881 787 743 597 393 337 268
107 100 48
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2537 2537
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 4932 1678
Size: 3254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373
Size: 32 135 32 2854 32 42 32 2626
Fragments arranged by size:
2854 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2747 748
Size: 1999 3786
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3068 4899 5083 1305
Size: 1763 1831 184 2007
Fragments arranged by size:
2007 1831 1763 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2457 3743 4102 4261 518
Size: 648 1291 1286 359 159 2042
Fragments arranged by size:
2042 1291 1286 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2259 3019 3201 3878 5026 5271 1606
Size: 653 760 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 677 653 245 182
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5233
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2451 2807 3143 4688 1132
Size: 97 1222 356 336 1545 2229
Fragments arranged by size:
2229 1545 1222 356 336 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 2447 4684 2447
Size: 2237 3548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2438 1108
Size: 1330 4455
PfoI T'CCnGG_A
Cuts at: 2186 2391 2866 2186
Size: 205 475 5105
Fragments arranged by size:
5105 475 205
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspXI vC'TCGA_Gb
Cuts at: 2841 2841
Size: 5785
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2926 2958 2926
Size: 32 5753
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2259 3019 2259
Size: 760 5025
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 3133 4268 4949 5140 826
Size: 219 2088 1135 681 191 1471
Fragments arranged by size:
2088 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2841 3001 3889 4790 5052 820
Size: 197 926 898 160 888 901 262 1553
Fragments arranged by size:
1553 926 901 898 888 262 197 160
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 2779 152
Size: 2627 3158
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 2999 1517
Size: 1482 4303
StyI C'CwwG_G
Cuts at: 513 1065 3224 513
Size: 552 2159 3074
Fragments arranged by size:
3074 2159 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2296 2328 2914 2946 5745 5777 2072
Size: 32 192 32 586 32 2799 32 2080
Fragments arranged by size:
2799 2080 586 192 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 2841 1943
Size: 898 4887
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI