Restriction map for
pFN21AB6514
(Circular) (Six-base) MAPSORT of: pFN21AB6514.seq Check: 7462 from: 1 to: 5818
pFN21AB6514 5818 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2880 3161 1937
Size: 943 281 4594
Fragments arranged by size:
4594 943 281
Acc65I G'GTAC_C
Cuts at: 3057 3139 3057
Size: 82 5736
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 4717 5694 1203
Size: 3514 977 1327
Fragments arranged by size:
3514 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 2528 1166
Size: 1362 4456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2109 2680 2861 3561 5133 2109
Size: 571 181 700 1572 2794
Fragments arranged by size:
2794 1572 700 571 181
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 2201 3127 3353 3505 5614 1493
Size: 708 926 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 708 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 3143 1870
Size: 73 1200 4545
Fragments arranged by size:
4545 1200 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2473 2506 2473
Size: 33 5785
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3057 3139 4594 618
Size: 325 217 627 1270 82 1455 1842
Fragments arranged by size:
1842 1455 1270 627 325 217 82
BanII G_rGCy'C
Cuts at: 1391 2396 3137 1391
Size: 1005 741 4072
Fragments arranged by size:
4072 1005 741
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2977 3011 4009 4043 1903
Size: 34 1040 34 998 34 3678
Fragments arranged by size:
3678 1040 998 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 2515 1548
Size: 967 4851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4429 136
Size: 107 122 71 631 605 2757 1525
Fragments arranged by size:
2757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5813 5813
Size: 5818
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 3879 5035 1790
Size: 2089 1156 2573
Fragments arranged by size:
2573 2089 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2871 3549 4507
Size: 416 53 83 399 24 1454 678 958
Cuts at: 4507 442
Size: 1753
Fragments arranged by size:
1753 1454 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2339 2483 4478 1239
Size: 838 262 144 1995 2579
Fragments arranged by size:
2579 1995 838 262 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2380 4481 882
Size: 617 881 2101 2219
Fragments arranged by size:
2219 2101 881 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3625 4007 4642 275
Size: 53 83 186 936 2092 382 635 1451
Fragments arranged by size:
2092 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2212 2242 2337 2367 3060
Size: 30 165 30 809 30 95 30 693
Cuts at: 3060 3090 1178
Size: 30 3906
Fragments arranged by size:
3906 809 693 165 95 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5818
BseYI C'CCAG_C
Cuts at: 2612 2657 2688 5021 5517 2612
Size: 45 31 2333 496 2913
Fragments arranged by size:
2913 2333 496 45 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2488 3268 4029 5057 1540
Size: 275 186 487 780 761 1028 2301
Fragments arranged by size:
2301 1028 780 761 487 275 186
BsiHKAI G_wGCw'C
Cuts at: 2396 3879 3964 5035 2396
Size: 1483 85 1071 3179
Fragments arranged by size:
3179 1483 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 3137 3879 3964 5035 1391
Size: 399 606 741 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 606 399 85
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 844
Size: 2329 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2089 4313 4487 65
Size: 1183 841 2224 174 1396
Fragments arranged by size:
2224 1396 1183 841 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2709 4462 4649 1700
Size: 110 899 1753 187 2869
Fragments arranged by size:
2869 1753 899 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2542 2542
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 3148 3515 3900 3917 5369 5455 5467 5813
Size: 1176 367 385 17 1452 86 12 346
Cuts at: 5813 1972
Size: 1977
Fragments arranged by size:
1977 1452 1176 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2049 2049
Size: 5818
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 3033 513
Size: 552 939 36 993 3298
Fragments arranged by size:
3298 993 939 552 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3025 487
Size: 683 56 1799 3280
Fragments arranged by size:
3280 1799 683 56
BtsI GCAGTG_nn'
Cuts at: 2949 3334 4128 4148 2949
Size: 385 794 20 4619
Fragments arranged by size:
4619 794 385 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2811 2846 529
Size: 35 2247 35 3501
Fragments arranged by size:
3501 2247 35 35
DraI TTT'AAA
Cuts at: 2340 3094 3122 2340
Size: 754 28 5036
Fragments arranged by size:
5036 754 28
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2485 2602 3030
Size: 54 1425 50 275 18 655 117 428
Cuts at: 3030 3036 3265 4154 5783 8
Size: 6 229 889 1629 43
Fragments arranged by size:
1629 1425 889 655 428 275 229 117
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2485 3265 1537
Size: 275 673 780 4090
Fragments arranged by size:
4090 780 673 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3748 1353
Size: 866 1529 3423
Fragments arranged by size:
3423 1529 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2394 2394
Size: 5818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2339 2483 3881 4478 5265 5533
Size: 393 445 262 144 1398 597 787 268
Cuts at: 5533 1239
Size: 1524
Fragments arranged by size:
1524 1398 787 597 445 393 268 262
144
EcoNI CCTnn'n_nnAGG
Cuts at: 2404 2404
Size: 5818
EcoO109I rG'GnC_Cy
Cuts at: 2677 2677
Size: 5818
EcoRI G'AATT_C
Cuts at: 2201 3127 3505 2201
Size: 926 378 4514
Fragments arranged by size:
4514 926 378
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2716 4965 1678
Size: 1038 2249 2531
Fragments arranged by size:
2531 2249 1038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 4459 4491 4533
Size: 32 135 32 765 32 2090 32 42
Cuts at: 4533 4565 1373
Size: 32 2626
Fragments arranged by size:
2626 2090 765 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 2296 3162 3408 669
Size: 988 281 232 126 866 246 3079
Fragments arranged by size:
3079 988 866 281 246 232 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 2296 3408 2296
Size: 1112 4706
KpnI G_GTAC'C
Cuts at: 3061 3143 3061
Size: 82 5736
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2090 4932 5116 1305
Size: 785 2842 184 2007
Fragments arranged by size:
2842 2007 785 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2604 3038 10
Size: 54 1425 343 772 434 2790
Fragments arranged by size:
2790 1425 772 434 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3776 4135 4294 518
Size: 648 1362 1248 359 159 2042
Fragments arranged by size:
2042 1362 1248 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2616 3234 3911 5059 5304 1606
Size: 1010 618 677 1148 245 2120
Fragments arranged by size:
2120 1148 1010 677 618 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2040 3033 513
Size: 552 939 36 993 3298
Fragments arranged by size:
3298 993 939 552 36
NdeI CA'TA_TG
Cuts at: 387 2380 387
Size: 1993 3825
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4916
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PstI C_TGCA'G
Cuts at: 830 3170 830
Size: 2340 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
SacI G_AGCT'C
Cuts at: 2396 2396
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 3166 4301 4982 5173 826
Size: 219 2121 1135 681 191 1471
Fragments arranged by size:
2121 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 3145 1872
Size: 1273 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3922 4823 5085 820
Size: 197 926 1979 901 262 1553
Fragments arranged by size:
1979 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 3033 3257 513
Size: 552 939 36 993 224 3074
Fragments arranged by size:
3074 993 939 552 224 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2853 3845 3862 4015 4200 4645 325
Size: 1255 1273 992 17 153 185 445 1498
Fragments arranged by size:
1498 1273 1255 992 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4064 5776 96
Size: 274 80 33 51 1443 2087 1712 138
Fragments arranged by size:
2087 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 3060 3092 5778 5810 2212
Size: 32 816 32 2686 32 2220
Fragments arranged by size:
2686 2220 816 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5818
XmaI C'CCGG_G
Cuts at: 1870 3143 1870
Size: 1273 4545
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI