Restriction map for
pFN21AB6512
(Circular) (Six-base) MAPSORT of: pFN21AB6512.seq Check: 8618 from: 1 to: 5857
pFN21AB6512 5857 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2065 3006 3200 1937
Size: 128 941 194 4594
Fragments arranged by size:
4594 941 194 128
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2523 2874 3920 5304 5572 1632
Size: 891 351 1046 1384 268 1917
Fragments arranged by size:
1917 1384 1046 891 351 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 2434 2824 4586 2434
Size: 390 1762 3705
Fragments arranged by size:
3705 1762 390
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2181 2438 3600 5172 2181
Size: 257 1162 1572 2866
Fragments arranged by size:
2866 1572 1162 257
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 1870
Size: 73 1239 4545
Fragments arranged by size:
4545 1239 73
BamHI G'GATC_C
Cuts at: 2306 2867 3187 3554 2306
Size: 561 320 367 4609
Fragments arranged by size:
4609 561 367 320
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3178 4633 618
Size: 325 217 627 1391 1455 1842
Fragments arranged by size:
1842 1455 1391 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2803 3085 3176 1391
Size: 1412 282 91 4072
Fragments arranged by size:
4072 1412 282 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2962 928
Size: 971 1063 3823
Fragments arranged by size:
3823 1063 971
BbvCI CC'TCA_GC
Cuts at: 2742 3102 2742
Size: 360 5497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 2188 3918 5074 1790
Size: 398 1730 1156 2573
Fragments arranged by size:
2573 1730 1156 398
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2753 3088 3588
Size: 416 53 83 399 24 1336 335 500
Cuts at: 3588 4546 442
Size: 958 1753
Fragments arranged by size:
1753 1336 958 500 416 399 335 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2814 2918 2923 3069 4517 1239
Size: 893 682 104 5 146 1448 2579
Fragments arranged by size:
2579 1448 893 682 146 104 5
Bpu10I CC'TnA_GC
Cuts at: 2742 3102 3111 2742
Size: 360 9 5488
Fragments arranged by size:
5488 360 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2403 2433 2896 2926 1178
Size: 30 165 30 1000 30 463 30 4109
Fragments arranged by size:
4109 1000 463 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2759 1186
Size: 1573 4284
BseYI C'CCAG_C
Cuts at: 3144 5060 5556 3144
Size: 1916 496 3445
Fragments arranged by size:
3445 1916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3918 4003 5074 3918
Size: 85 1071 4701
Fragments arranged by size:
4701 1071 85
BsmI GAATG_Cn'
Cuts at: 2246 3368 3461 2246
Size: 1122 93 4642
Fragments arranged by size:
4642 1122 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2803 3085 3176 3918 4003 5074
Size: 399 398 615 282 91 742 85 1071
Cuts at: 5074 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 615 399 398 282 91
85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3212 844
Size: 2368 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2421 96
Size: 2325 3532
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2447 794
Size: 1653 4204
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 2395 2795 2395
Size: 400 5457
BstYI r'GATC_y
Cuts at: 1972 2073 2306 2867 3187 3554 3939 3956 5408
Size: 101 233 561 320 367 385 17 1452
Cuts at: 5408 5494 5506 5852 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 561 385 367 346 320 233
101 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DraIII CAC_nnn'GTG
Cuts at: 2914 2914
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3304 4193 5822
Size: 54 1425 50 275 18 1474 889 1629
Cuts at: 5822 8
Size: 43
Fragments arranged by size:
1629 1474 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2523 2814 2874 2918 2923 3069
Size: 393 500 391 291 60 44 5 146
Cuts at: 3069 3920 4517 5304 5572 1239
Size: 851 597 787 268 1524
Fragments arranged by size:
1524 851 787 597 500 393 391 291
268 146 60 44 5
EcoO109I rG'GnC_Cy
Cuts at: 2178 2178
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2555 2555
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2058 2085 2090 2311
Size: 32 135 32 481 5 27 5 221
Cuts at: 2311 2343 2420 2452 2810 2828 2842 2860 4498
Size: 32 77 32 358 18 14 18 1638
Cuts at: 4498 4530 4572 4604 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1638 481 358 221 135 77 42
32 32 32 32 32 32 27 18
18 14 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4035
Fragments arranged by size:
4035 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 2393 2793 3815 4174 4333 518
Size: 648 1126 101 400 1022 359 159 2042
Fragments arranged by size:
2042 1126 1022 648 400 359 159 101
MspA1I CmG'CkG
Cuts at: 1606 2834 2874 3273 3950 5098 5343 1606
Size: 1228 40 399 677 1148 245 2120
Fragments arranged by size:
2120 1228 1148 677 399 245 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3215 4760 1132
Size: 97 1986 1545 2229
Fragments arranged by size:
2229 1986 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2339 1108
Size: 1231 4626
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PstI C_TGCA'G
Cuts at: 830 3209 830
Size: 2379 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2834 2874 2834
Size: 40 5817
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SexAI A'CCwGG_T
Cuts at: 2332 2820 2332
Size: 488 5369
SfcI C'TryA_G
Cuts at: 826 1045 3125 3205 4340 5021 5212 826
Size: 219 2080 80 1135 681 191 1471
Fragments arranged by size:
2080 1471 1135 681 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 3027 1517
Size: 1510 4347
StyI C'CwwG_G
Cuts at: 513 1065 2004 3296 513
Size: 552 939 1292 3074
Fragments arranged by size:
3074 1292 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 3884 3901 4054 4239 4684 325
Size: 1255 1497 807 17 153 185 445 1498
Fragments arranged by size:
1498 1497 1255 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 4103 5815
Size: 274 80 33 51 1443 444 1682 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1682 1443 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 2896 2928 5817 5849 2403
Size: 32 461 32 2889 32 2411
Fragments arranged by size:
2889 2411 461 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 2980
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI