Restriction map for
pFN21AB6510
(Circular) (Six-base) MAPSORT of: pFN21AB6510.seq Check: 704 from: 1 to: 5857
pFN21AB6510 5857 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2564 2691 3920 5304 5572 1632
Size: 431 501 127 1229 1384 268 1917
Fragments arranged by size:
1917 1384 1229 501 431 268 127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 4756 5733 1203
Size: 3553 977 1327
Fragments arranged by size:
3553 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2301 2895 1184
Size: 1117 594 4146
Fragments arranged by size:
4146 1117 594
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 2679
Size: 32 5825
AlwNI CAG_nnn'CTG
Cuts at: 2555 3600 5172 2555
Size: 1045 1572 3240
Fragments arranged by size:
3240 1572 1045
ApaLI G'TGCA_C
Cuts at: 2226 3914 5070 2226
Size: 1688 1156 3013
Fragments arranged by size:
3013 1688 1156
ApoI r'AATT_y
Cuts at: 1493 2114 2143 3166 3392 3544 5653 1493
Size: 621 29 1023 226 152 2109 1697
Fragments arranged by size:
2109 1697 1023 621 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 3182 1870
Size: 73 1239 4545
Fragments arranged by size:
4545 1239 73
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2972 3178 4633 618
Size: 325 217 627 1185 206 1455 1842
Fragments arranged by size:
1842 1455 1185 627 325 217 206
BanII G_rGCy'C
Cuts at: 1391 2112 2985 3176 1391
Size: 721 873 191 4072
Fragments arranged by size:
4072 873 721 191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 4468 136
Size: 107 122 71 631 605 700 2096 1525
Fragments arranged by size:
2096 1525 700 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2176 3022 5852 2176
Size: 846 2830 2181
Fragments arranged by size:
2830 2181 846
BlpI GC'TnA_GC
Cuts at: 2268 3274 2268
Size: 1006 4851
Bme1580I G_kGCm'C
Cuts at: 1790 2230 2775 3918 5074 1790
Size: 440 545 1143 1156 2573
Fragments arranged by size:
2573 1156 1143 545 440
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 3588 4546
Size: 416 53 83 399 24 912 1259 958
Cuts at: 4546 442
Size: 1753
Fragments arranged by size:
1753 1259 958 912 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2756 4517 1239
Size: 1055 462 1761 2579
Fragments arranged by size:
2579 1761 1055 462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 2688 5851 2688
Size: 3163 2694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4290 4688 4962 5109 5520 4290
Size: 398 274 147 411 4627
Fragments arranged by size:
4627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 2901 2931 1178
Size: 30 165 30 1374 30 94 30 4104
Fragments arranged by size:
4104 1374 165 94 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2819 2822 1186
Size: 1009 624 3 4221
Fragments arranged by size:
4221 1009 624 3
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2568 2891 1163
Size: 573 450 382 323 4129
Fragments arranged by size:
4129 573 450 382 323
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2230 2985 3918 4003 5074 2230
Size: 755 933 85 1071 3013
Fragments arranged by size:
3013 1071 933 755 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2230 2775 2985 3176 3918 4003
Size: 399 322 118 545 210 191 742 85
Cuts at: 4003 5074 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 322 210 191
118 85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 2800 3746 5476 2800
Size: 946 1730 3181
Fragments arranged by size:
3181 1730 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 3212 844
Size: 2095 273 3489
Fragments arranged by size:
3489 2095 273
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3917 4929 3917
Size: 1012 4845
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2176 2649 3022 3187 3554 3939 3956 5408
Size: 204 473 373 165 367 385 17 1452
Cuts at: 5408 5494 5506 5852 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 473 385 373 367 346 204
165 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2069 513
Size: 552 939 65 4301
Fragments arranged by size:
4301 939 552 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 4167 4187 3373
Size: 794 20 5043
Fragments arranged by size:
5043 794 20
ClaI AT'CG_AT
Cuts at: 1554 2080 1554
Size: 526 5331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2183 2341 2988
Size: 54 1425 50 275 18 353 158 647
Cuts at: 2988 3111 3304 4193 5822 8
Size: 123 193 889 1629 43
Fragments arranged by size:
1629 1425 889 647 353 275 193 158
123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 3787 1353
Size: 1314 1120 3423
Fragments arranged by size:
3423 1314 1120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2983 2983
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2294 2564 2691 2756 3920 4517
Size: 393 431 231 270 127 65 1164 597
Cuts at: 4517 5304 5572 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1164 787 597 431 393 270 268
231 127 65
EcoNI CCTnn'n_nnAGG
Cuts at: 2801 2801
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
EcoRV GAT'ATC
Cuts at: 2419 2419
Size: 5857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2891 2891
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2403 2435 2471 2503 2777
Size: 32 135 32 831 32 36 32 274
Cuts at: 2777 2809 4498 4530 4572 4604 1373
Size: 32 1689 32 42 32 2626
Fragments arranged by size:
2626 1689 831 274 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2185 2343 2990 3113 10
Size: 54 1425 343 353 158 647 123 2754
Fragments arranged by size:
2754 1425 647 353 343 158 123 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2262 2661 3815 4174 4333 518
Size: 648 1096 399 1154 359 159 2042
Fragments arranged by size:
2042 1154 1096 648 399 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2281 3273 3950 5098 5343 1606
Size: 465 210 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 465 245 210
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NruI TCG'CGA
Cuts at: 2060 2060
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 3215 4760 1132
Size: 97 1986 1545 2229
Fragments arranged by size:
2229 1986 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PfoI T'CCnGG_A
Cuts at: 2031 2031
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2331 2353 2381 2520 3209 830
Size: 1501 22 28 139 689 3478
Fragments arranged by size:
3478 1501 689 139 28 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2281 2281
Size: 5857
SacI G_AGCT'C
Cuts at: 2985 2985
Size: 5857
SacII CC_GC'GG
Cuts at: 2072 2072
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 2468 4105 5817 2468
Size: 1637 1712 2508
Fragments arranged by size:
2508 1712 1637
SfcI C'TryA_G
Cuts at: 826 1045 2327 2349 2377 2516 2642 3205 4340
Size: 219 1282 22 28 139 126 563 1135
Cuts at: 4340 5021 5212 826
Size: 681 191 1471
Fragments arranged by size:
1471 1282 1135 681 563 219 191 139
126 28 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 3296 513
Size: 552 939 285 1007 3074
Fragments arranged by size:
3074 1007 939 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2444 3884 3901 4054 4239 4684 325
Size: 1255 864 1440 17 153 185 445 1498
Fragments arranged by size:
1498 1440 1255 864 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 3129 4103
Size: 274 80 33 51 1443 489 663 974
Cuts at: 4103 5815 96
Size: 1712 138
Fragments arranged by size:
1712 1443 974 663 489 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2983 2983
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI