Restriction map for
pFN21AB6508
(Circular) (Six-base) MAPSORT of: pFN21AB6508.seq Check: 4769 from: 1 to: 5857
pFN21AB6508 5857 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 2304 3178 2304
Size: 874 4983
AclI AA'CG_TT
Cuts at: 1563 2223 3984 4357 1563
Size: 660 1761 373 3063
Fragments arranged by size:
3063 1761 660 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 2279 2755 4756 5733 1203
Size: 1076 476 2001 977 1327
Fragments arranged by size:
2001 1327 1076 977 476
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2919 3600 5172 2919
Size: 681 1572 3604
Fragments arranged by size:
3604 1572 681
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 3182 1870
Size: 73 170 1069 4545
Fragments arranged by size:
4545 1069 170 73
BamHI G'GATC_C
Cuts at: 2118 3187 3554 2118
Size: 1069 367 4421
Fragments arranged by size:
4421 1069 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2304 3178 4633 618
Size: 325 217 627 517 874 1455 1842
Fragments arranged by size:
1842 1455 874 627 517 325 217
BanII G_rGCy'C
Cuts at: 1391 3176 1391
Size: 1785 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 2647 928
Size: 971 481 267 4138
Fragments arranged by size:
4138 971 481 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 2187 3036 1548
Size: 639 849 4369
Fragments arranged by size:
4369 849 639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 3030 4468
Size: 107 122 71 631 605 500 858 1438
Cuts at: 4468 136
Size: 1525
Fragments arranged by size:
1525 1438 858 631 605 500 122 107
71
BglII A'GATC_T
Cuts at: 2193 2472 5852 2193
Size: 279 3380 2198
Fragments arranged by size:
3380 2198 279
BlpI GC'TnA_GC
Cuts at: 2978 3274 2978
Size: 296 5561
Bme1580I G_kGCm'C
Cuts at: 1790 2596 2926 3918 5074 1790
Size: 806 330 992 1156 2573
Fragments arranged by size:
2573 1156 992 806 330
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2282
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2271 4517 1239
Size: 814 218 2246 2579
Fragments arranged by size:
2579 2246 814 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3071 3982 4847 5145 3071
Size: 911 865 298 3783
Fragments arranged by size:
3783 911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2253 3051 4520 882
Size: 617 754 798 1469 2219
Fragments arranged by size:
2219 1469 798 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 2192 2471 5851 2192
Size: 279 3380 2198
Fragments arranged by size:
3380 2198 279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3042 4290 4688 4962 5109 5520 3042
Size: 1248 398 274 147 411 3379
Fragments arranged by size:
3379 1248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2269 2299 1178
Size: 30 165 30 866 30 4736
Fragments arranged by size:
4736 866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 1186
Size: 1072 4785
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2287 3918 4003 5074 2287
Size: 1631 85 1071 3070
Fragments arranged by size:
3070 1631 1071 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2596 2926 3176 3918 4003 5074
Size: 399 497 309 330 250 742 85 1071
Cuts at: 5074 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 497 399 330 309 250
85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2292 2982 3080 3212 844
Size: 1427 21 690 98 132 3489
Fragments arranged by size:
3489 1427 690 132 98 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2865 3148 3744 1394
Size: 429 1042 283 596 3507
Fragments arranged by size:
3507 1042 596 429 283
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 4352 4526 65
Size: 1183 1452 1652 174 1396
Fragments arranged by size:
1652 1452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 2201 96
Size: 2105 3752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 3143 3917 4929 3143
Size: 774 1012 4071
Fragments arranged by size:
4071 1012 774
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2083 2118 2193 2472 3187 3554 3939 3956
Size: 111 35 75 279 715 367 385 17
Cuts at: 3956 5408 5494 5506 5852 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 715 385 367 346 279 111
86 75 35 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2739 3105 513
Size: 552 939 735 366 3265
Fragments arranged by size:
3265 939 735 552 366
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 3373 4167 4187 2056
Size: 1317 794 20 3726
Fragments arranged by size:
3726 1317 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2435 2517 3108
Size: 54 1425 50 275 18 605 82 591
Cuts at: 3108 3304 4193 5822 8
Size: 196 889 1629 43
Fragments arranged by size:
1629 1425 889 605 591 275 196 82
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3787 1353
Size: 2434 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2271 3920 4517 5304 5572 1239
Size: 393 421 218 1649 597 787 268 1524
Fragments arranged by size:
1649 1524 787 597 421 393 268 218
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
EcoRV GAT'ATC
Cuts at: 2446 2446
Size: 5857
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2405 5004 1678
Size: 727 2599 2531
Fragments arranged by size:
2599 2531 727
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 4498 4530 4572
Size: 32 135 32 518 32 2376 32 42
Cuts at: 4572 4604 1373
Size: 32 2626
Fragments arranged by size:
2626 2376 518 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 2308 3182 2308
Size: 874 4983
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 3110 10
Size: 54 1425 343 687 591 2757
Fragments arranged by size:
2757 1425 687 591 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 2610 2894 3815 4174 4333 518
Size: 648 1341 103 284 921 359 159 2042
Fragments arranged by size:
2042 1341 921 648 359 284 159 103
MspA1I CmG'CkG
Cuts at: 1606 3013 3273 3950 5098 5343 1606
Size: 1407 260 677 1148 245 2120
Fragments arranged by size:
2120 1407 1148 677 260 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2759 3215 4760 1132
Size: 97 1530 456 1545 2229
Fragments arranged by size:
2229 1545 1530 456 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5857
PciI A'CATG_T
Cuts at: 2755 4756 2755
Size: 2001 3856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2638 1108
Size: 1530 4327
PfoI T'CCnGG_A
Cuts at: 2777 2777
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2140 3209 830
Size: 1310 1069 3478
Fragments arranged by size:
3478 1310 1069
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 3013 3013
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2136 3205 4340 5021 5212 826
Size: 219 1091 1069 1135 681 191 1471
Fragments arranged by size:
1471 1135 1091 1069 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2115 3184 1872
Size: 243 1069 4545
Fragments arranged by size:
4545 1069 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3086 3961 4862 5124 820
Size: 197 926 1143 875 901 262 1553
Fragments arranged by size:
1553 1143 926 901 875 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 2166 2453 1517
Size: 649 287 4921
Fragments arranged by size:
4921 649 287
StyI C'CwwG_G
Cuts at: 513 1065 2004 3296 513
Size: 552 939 1292 3074
Fragments arranged by size:
3074 1292 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 3901 4054 4239 4684 325
Size: 1255 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2201 3065 4103
Size: 274 80 33 51 1443 224 864 1038
Cuts at: 4103 5815 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1038 864 274 224 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5817 5849 5817
Size: 32 5825
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2305 2800 3103 2251
Size: 54 495 303 5005
Fragments arranged by size:
5005 495 303 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2113 3182 1870
Size: 243 1069 4545
Fragments arranged by size:
4545 1069 243
XmnI GAAnn'nnTTC
Cuts at: 2999 3986 2999
Size: 987 4870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI