Restriction map for
pFN21AB6505
(Circular) (Six-base) MAPSORT of: pFN21AB6505.seq Check: 384 from: 1 to: 5779
pFN21AB6505 5779 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2285 3122 1937
Size: 348 837 4594
Fragments arranged by size:
4594 837 348
Acc65I G'GTAC_C
Cuts at: 2390 3100 2390
Size: 710 5069
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3842 5226 5494 1632
Size: 2210 1384 268 1917
Fragments arranged by size:
2210 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2664 4678 5655 1203
Size: 1461 2014 977 1327
Fragments arranged by size:
2014 1461 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2951 1184
Size: 1767 4012
AlwNI CAG_nnn'CTG
Cuts at: 2260 2279 3522 5094 2260
Size: 19 1243 1572 2945
Fragments arranged by size:
2945 1572 1243 19
ApaI G_GGCC'C
Cuts at: 2121 2214 2121
Size: 93 5686
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2716 3104 1870
Size: 73 288 485 388 4545
Fragments arranged by size:
4545 485 388 288 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 3100 4555 618
Size: 325 217 627 603 710 1455 1842
Fragments arranged by size:
1842 1455 710 627 603 325 217
BanII G_rGCy'C
Cuts at: 1391 2110 2121 2159 2214 2232 2334 3098 1391
Size: 719 11 38 55 18 102 764 4072
Fragments arranged by size:
4072 764 719 102 55 38 18 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 928
Size: 971 664 4144
Fragments arranged by size:
4144 971 664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2348 2382 2838 2872 3970
Size: 34 50 34 327 34 456 34 1098
Cuts at: 3970 4004 1903
Size: 34 3678
Fragments arranged by size:
3678 1098 456 327 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2474 3671 4881 2474
Size: 1197 1210 3372
Fragments arranged by size:
3372 1210 1197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 2274 4390
Size: 107 122 71 631 605 583 19 2116
Cuts at: 4390 136
Size: 1525
Fragments arranged by size:
2116 1525 631 605 583 122 107 71
19
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2214 2672 3840 4996 1790
Size: 331 93 458 1168 1156 2573
Fragments arranged by size:
2573 1168 1156 458 331 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 3032 3510
Size: 416 53 83 399 24 1226 389 478
Cuts at: 3510 4468 442
Size: 958 1753
Fragments arranged by size:
1753 1226 958 478 416 399 389 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2246 2348 2624 2633 2758 4439 1239
Size: 915 92 102 276 9 125 1681 2579
Fragments arranged by size:
2579 1681 915 276 125 102 92 9
Bpu10I CC'TnA_GC
Cuts at: 2153 2469 2968 2153
Size: 316 499 4964
Fragments arranged by size:
4964 499 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 3904 4769 5067 2537
Size: 1367 865 298 3249
Fragments arranged by size:
3249 1367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2680 3570 493
Size: 711 1476 890 2702
Fragments arranged by size:
2702 1476 890 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3968 4603 275
Size: 53 83 186 936 2053 382 635 1451
Fragments arranged by size:
2053 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2390 3062 1186
Size: 1063 141 672 3903
Fragments arranged by size:
3903 1063 672 141
BseYI C'CCAG_C
Cuts at: 2121 2394 2671 4982 5478 2121
Size: 273 277 2311 496 2422
Fragments arranged by size:
2422 2311 496 277 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 2921 1163
Size: 573 510 675 4021
Fragments arranged by size:
4021 675 573 510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3229 3990 5018 1540
Size: 275 186 1228 761 1028 2301
Fragments arranged by size:
2301 1228 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2110 2490 3840 3925 4996 2110
Size: 380 1350 85 1071 2893
Fragments arranged by size:
2893 1350 1071 380 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2121 2159 2214 2232 2334 2490
Size: 399 320 11 38 55 18 102 156
Cuts at: 2490 2672 3098 3840 3925 4996 1391
Size: 182 426 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 320 182 156
102 85 55 38 18 11
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2297 3134 844
Size: 1453 837 3489
Fragments arranged by size:
3489 1453 837
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2771 4423 4610 1700
Size: 110 961 1652 187 2869
Fragments arranged by size:
2869 1652 961 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2020 2512 2020
Size: 492 5287
BstYI r'GATC_y
Cuts at: 1972 2820 3109 3476 3861 3878 5330 5416 5428
Size: 848 289 367 385 17 1452 86 12
Cuts at: 5428 5774 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 848 385 367 346 289 86
17 12
BstZ17I GTA'TAC
Cuts at: 2286 2286
Size: 5779
Bsu36I CC'TnA_GG
Cuts at: 2475 2475
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2425 2827 513
Size: 552 1360 402 3465
Fragments arranged by size:
3465 1360 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2193 2263 3295 4089 4109 2193
Size: 70 1032 794 20 3863
Fragments arranged by size:
3863 1032 794 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2169 2204 529
Size: 35 1605 35 4104
Fragments arranged by size:
4104 1605 35 35
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2319 2502
Size: 54 1425 50 275 18 360 129 183
Cuts at: 2502 2769 2773 3226 4115 5744 8
Size: 267 4 453 889 1629 43
Fragments arranged by size:
1629 1425 889 453 360 275 267 183
129 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 2762 2816 3039 3709 1353
Size: 1142 267 54 223 670 3423
Fragments arranged by size:
3423 1142 670 267 223 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 2813 4366 4740 4886 387
Size: 1064 138 894 330 1553 374 146 1280
Fragments arranged by size:
1553 1280 1064 894 374 330 146 138
EcoICRI GAG'CTC
Cuts at: 2108 2108
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2246 2348 2624 2633 2758 3842
Size: 393 522 92 102 276 9 125 1084
Cuts at: 3842 4439 5226 5494 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1084 787 597 522 393 276 268
125 102 92 9
EcoO109I rG'GnC_Cy
Cuts at: 2118 2211 2257 2380 2684 2832 2956 2118
Size: 93 46 123 304 148 124 4941
Fragments arranged by size:
4941 304 148 124 123 93 46
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FseI GG_CCGG'CC
Cuts at: 2775 2775
Size: 5779
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2756 4926 1678
Size: 1078 2170 2531
Fragments arranged by size:
2531 2170 1078
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2973 3005 4420 4452 4494
Size: 32 135 32 1401 32 1415 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 1415 1401 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KpnI G_GTAC'C
Cuts at: 2394 3104 2394
Size: 710 5069
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 2664 5655 2664
Size: 2991 2788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 2046 4893 5077 1305
Size: 735 6 2847 184 2007
Fragments arranged by size:
2847 2007 735 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2504 10
Size: 54 1425 343 360 312 3285
Fragments arranged by size:
3285 1425 360 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 3737 4096 4255 518
Size: 648 1192 1379 359 159 2042
Fragments arranged by size:
2042 1379 1192 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2829 2871 2906 3195 3872 5020 5265 1606
Size: 1223 42 35 289 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 677 289 245 42 35
NaeI GCC'GGC
Cuts at: 2773 2773
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 2425 513
Size: 552 1360 3867
Fragments arranged by size:
3867 1360 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2771 2771
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2363 2437 3137 4682 1132
Size: 97 1134 74 700 1545 2229
Fragments arranged by size:
2229 1545 1134 700 97 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PfoI T'CCnGG_A
Cuts at: 2045 2223 2307 2787 2045
Size: 178 84 480 5037
Fragments arranged by size:
5037 480 178 84
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3051 3083 3848 3880 5361 5393 3051
Size: 32 765 32 1481 32 3437
Fragments arranged by size:
3437 1481 765 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2684 2956 2380
Size: 304 272 5203
Fragments arranged by size:
5203 304 272
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2117 2210 2117
Size: 93 5686
PstI C_TGCA'G
Cuts at: 830 2851 3131 830
Size: 2021 280 3478
Fragments arranged by size:
3478 2021 280
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2906 2906
Size: 5779
SacI G_AGCT'C
Cuts at: 2110 2110
Size: 5779
SacII CC_GC'GG
Cuts at: 2830 2830
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2495 2762 2816 2495
Size: 267 54 5458
Fragments arranged by size:
5458 267 54
SbfI CC_TGCA'GG
Cuts at: 2851 3131 2851
Size: 280 5499
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2271 2847 3127 4262 4943 5134 826
Size: 219 1226 576 280 1135 681 191 1471
Fragments arranged by size:
1471 1226 1135 681 576 280 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2255 2255
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3883 4784 5046 820
Size: 197 926 609 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 2853 1517
Size: 1336 4443
StyI C'CwwG_G
Cuts at: 513 1065 2425 3218 513
Size: 552 1360 793 3074
Fragments arranged by size:
3074 1360 793 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4025 5737 96
Size: 274 80 33 51 1443 2048 1712 138
Fragments arranged by size:
2048 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2380
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3066 3908 3066
Size: 842 4937
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI DraIII EcoNI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SexAI SfoI SrfI SwaI