Restriction map for
pFN21AB6503
(Circular) (Six-base) MAPSORT of: pFN21AB6503.seq Check: 1324 from: 1 to: 5779
pFN21AB6503 5779 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 2670 3100 2670
Size: 430 5349
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2025 3842 5226 5494 1632
Size: 393 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 393 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2862 4678 5655 1203
Size: 1659 1816 977 1327
Fragments arranged by size:
1816 1659 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 5747
AlwNI CAG_nnn'CTG
Cuts at: 2430 2501 2761 3522 5094 2430
Size: 71 260 761 1572 3115
Fragments arranged by size:
3115 1572 761 260 71
ApaLI G'TGCA_C
Cuts at: 2313 3836 4992 2313
Size: 1523 1156 3100
Fragments arranged by size:
3100 1523 1156
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 3104 1870
Size: 73 1161 4545
Fragments arranged by size:
4545 1161 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2670 3100 4555 618
Size: 325 217 627 883 430 1455 1842
Fragments arranged by size:
1842 1455 883 627 430 325 217
BanII G_rGCy'C
Cuts at: 1391 2814 3098 1391
Size: 1423 284 4072
Fragments arranged by size:
4072 1423 284
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2644 2906 928
Size: 971 745 262 3801
Fragments arranged by size:
3801 971 745 262
BbvCI CC'TCA_GC
Cuts at: 2010 2010
Size: 5779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 3671 4881 2888
Size: 783 1210 3786
Fragments arranged by size:
3786 1210 783
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BfrBI ATG'CAT
Cuts at: 2286 2286
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4390 136
Size: 107 122 71 631 605 2718 1525
Fragments arranged by size:
2718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2317 3840 4996 1790
Size: 520 7 1523 1156 2573
Fragments arranged by size:
2573 1523 1156 520 7
BmgBI CAC'GTC
Cuts at: 1318 3072 1318
Size: 1754 4025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2370 2678 2828 2891 3029 4439 1239
Size: 801 330 308 150 63 138 1410 2579
Fragments arranged by size:
2579 1410 801 330 308 150 138 63
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2696 3904 4769 5067 2696
Size: 1208 865 298 3408
Fragments arranged by size:
3408 1208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 4442 882
Size: 617 1485 1458 2219
Fragments arranged by size:
2219 1485 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3968 4603 275
Size: 53 83 186 936 2053 382 635 1451
Fragments arranged by size:
2053 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 1178
Size: 30 165 30 795 30 4729
Fragments arranged by size:
4729 795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2373 1186
Size: 874 313 4592
Fragments arranged by size:
4592 874 313
BseYI C'CCAG_C
Cuts at: 4982 5478 4982
Size: 496 5283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3229 3990 5018 1540
Size: 275 186 1228 761 1028 2301
Fragments arranged by size:
2301 1228 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2317 2625 2922 3840 3925 4996 2317
Size: 308 297 918 85 1071 3100
Fragments arranged by size:
3100 1071 918 308 297 85
BsmI GAATG_Cn'
Cuts at: 2288 3290 3383 2288
Size: 1002 93 4684
Fragments arranged by size:
4684 1002 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2317 2625 2814 2922 3098 3840
Size: 399 520 7 308 189 108 176 742
Cuts at: 3840 3925 4996 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 308 189 176
108 85 7
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 2236 3668 5398 2236
Size: 1432 1730 2617
Fragments arranged by size:
2617 1730 1432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 844
Size: 2290 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 3666 1394
Size: 429 197 1646 3507
Fragments arranged by size:
3507 1646 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2720 4423 4610 1700
Size: 110 910 1703 187 2869
Fragments arranged by size:
2869 1703 910 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2091 3109 3476 3861 3878 5330 5416 5428
Size: 119 1018 367 385 17 1452 86 12
Cuts at: 5428 5774 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1018 385 367 346 119 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2508 2508
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2292 487
Size: 683 56 1066 3974
Fragments arranged by size:
3974 1066 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2536 2571 529
Size: 35 1972 35 3737
Fragments arranged by size:
3737 1972 35 35
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DraIII CAC_nnn'GTG
Cuts at: 2824 2824
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 3226 4115
Size: 54 1425 50 275 18 639 757 889
Cuts at: 4115 5744 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 757 639 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3709 1353
Size: 999 1357 3423
Fragments arranged by size:
3423 1357 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2040 2370 2678 2828 2891 3029
Size: 393 393 15 330 308 150 63 138
Cuts at: 3029 3842 4439 5226 5494 1239
Size: 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 597 393 393 330 308
268 150 138 63 15
EcoO109I rG'GnC_Cy
Cuts at: 2050 2427 2050
Size: 377 5402
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5779
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2949 4926 1678
Size: 1271 1977 2531
Fragments arranged by size:
2531 1977 1271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 4420 4452 4494
Size: 32 135 32 812 32 2004 32 42
Cuts at: 4494 4526 1373
Size: 32 2626
Fragments arranged by size:
2626 2004 812 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KpnI G_GTAC'C
Cuts at: 2674 3104 2674
Size: 430 5349
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2949 4893 5077 1305
Size: 1644 1944 184 2007
Fragments arranged by size:
2007 1944 1644 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 10
Size: 54 1425 343 639 3318
Fragments arranged by size:
3318 1425 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2987 3737 4096 4255 518
Size: 648 1821 750 359 159 2042
Fragments arranged by size:
2042 1821 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2498 2788 2932 3195 3872 5020 5265 1606
Size: 892 290 144 263 677 1148 245 2120
Fragments arranged by size:
2120 1148 892 677 290 263 245 144
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4288
Fragments arranged by size:
4288 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NsiI A_TGCA'T
Cuts at: 2288 2288
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2866 2992 3137 4682 1132
Size: 97 1637 126 145 1545 2229
Fragments arranged by size:
2229 1637 1545 145 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 2862 4678 2862
Size: 1816 3963
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2427 2427
Size: 5779
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PstI C_TGCA'G
Cuts at: 830 3131 830
Size: 2301 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2498 2788 2932 2498
Size: 290 144 5345
Fragments arranged by size:
5345 290 144
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2352 2352
Size: 5779
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SexAI A'CCwGG_T
Cuts at: 2429 2429
Size: 5779
SfcI C'TryA_G
Cuts at: 826 1045 3127 4262 4943 5134 826
Size: 219 2082 1135 681 191 1471
Fragments arranged by size:
2082 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2675 3883 4784 5046 820
Size: 197 926 732 1208 901 262 1553
Fragments arranged by size:
1553 1208 926 901 732 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 2338 152
Size: 2186 3593
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2004 3218 513
Size: 552 939 1214 3074
Fragments arranged by size:
3074 1214 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2814 3806 3823 3976 4161 4606 325
Size: 1255 1234 992 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1234 992 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 4025 5737
Size: 274 80 33 51 1443 228 1820 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1820 1712 1443 274 228 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2821 2821
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI