Restriction map for
pFN21AB6488
(Circular) (Six-base) MAPSORT of: pFN21AB6488.seq Check: 8723 from: 1 to: 5776
pFN21AB6488 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2408 2838 2408
Size: 430 5346
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3074 3839 5223 5491 1632
Size: 1442 765 1384 268 1917
Fragments arranged by size:
1917 1442 1384 765 268
AfeI AGC'GCT
Cuts at: 2105 2105
Size: 5776
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2870 1184
Size: 1686 4090
AlwNI CAG_nnn'CTG
Cuts at: 2877 3046 3519 5091 2877
Size: 169 473 1572 3562
Fragments arranged by size:
3562 1572 473 169
ApaLI G'TGCA_C
Cuts at: 2395 3833 4989 2395
Size: 1438 1156 3182
Fragments arranged by size:
3182 1438 1156
ApoI r'AATT_y
Cuts at: 1493 2369 2387 3085 3311 3463 5572 1493
Size: 876 18 698 226 152 2109 1697
Fragments arranged by size:
2109 1697 876 698 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 2159 2682 3101 1870
Size: 73 179 37 523 419 4545
Fragments arranged by size:
4545 523 419 179 73 37
AvrII C'CTAG_G
Cuts at: 2587 2587
Size: 5776
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 3097 4552 618
Size: 325 217 627 704 606 1455 1842
Fragments arranged by size:
1842 1455 704 627 606 325 217
BanII G_rGCy'C
Cuts at: 1391 3095 1391
Size: 1704 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3062 928
Size: 971 1163 3642
Fragments arranged by size:
3642 1163 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2028 2062 3967 4001 1903
Size: 34 50 34 7 34 1905 34 3678
Fragments arranged by size:
3678 1905 50 34 34 34 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2217 4387 136
Size: 107 122 71 631 605 545 2170 1525
Fragments arranged by size:
2170 1525 631 605 545 122 107 71
BglII A'GATC_T
Cuts at: 2233 5771 2233
Size: 3538 2238
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2135 2209 2399 2430 3837 4993 1790
Size: 333 12 74 190 31 1407 1156 2573
Fragments arranged by size:
2573 1407 1156 333 190 74 31 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 2555 3507
Size: 416 53 83 399 24 693 445 952
Cuts at: 3507 4465 442
Size: 958 1753
Fragments arranged by size:
1753 958 952 693 445 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2517 3023 3068 4436 1239
Size: 1019 259 506 45 1368 2579
Fragments arranged by size:
2579 1368 1019 506 259 45
Bpu10I CC'TnA_GC
Cuts at: 2457 2457
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2593 3901 4766 5064 2593
Size: 1308 865 298 3305
Fragments arranged by size:
3305 1308 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 3583 3965 4600
Size: 53 83 186 936 608 1442 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1451 1442 936 635 608 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 1178
Size: 30 165 30 1180 30 4341
Fragments arranged by size:
4341 1180 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 1186
Size: 1363 4413
BseYI C'CCAG_C
Cuts at: 2288 2470 4979 5475 2288
Size: 182 2509 496 2589
Fragments arranged by size:
2589 2509 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2293 2830 2840 2890 2980 1163
Size: 573 300 257 537 10 50 90 3959
Fragments arranged by size:
3959 573 537 300 257 90 50 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2399 2951 3837 3922 4993 2024
Size: 375 552 886 85 1071 2807
Fragments arranged by size:
2807 1071 886 552 375 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2123 2135 2209 2399 2430 2951
Size: 399 234 99 12 74 190 31 521
Cuts at: 2951 3095 3837 3922 4993 1391
Size: 144 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 521 399 234 190 144
99 85 74 31 12
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2159 2408 2838 3002 3131 844
Size: 1315 249 430 164 129 3489
Fragments arranged by size:
3489 1315 430 249 164 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 2167 1925
Size: 242 5534
BssSI C'ACGA_G
Cuts at: 2023 3836 4848 2023
Size: 1813 1012 2951
Fragments arranged by size:
2951 1813 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2014 794
Size: 1220 4556
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 2686 1184
Size: 1502 4274
BstYI r'GATC_y
Cuts at: 1972 2233 2517 3106 3473 3858 3875 5327 5413
Size: 261 284 589 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 589 385 367 346 284 261
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2051 2263 2365 2896 3292 4086 4106 2051
Size: 212 102 531 396 794 20 3721
Fragments arranged by size:
3721 794 531 396 212 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2644 2679 529
Size: 35 2080 35 3626
Fragments arranged by size:
3626 2080 35 35
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 2640 2640
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2612 4783 809
Size: 507 1296 2171 1802
Fragments arranged by size:
2171 1802 1296 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3223 4112
Size: 54 1425 50 275 18 1066 327 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1066 889 327 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2517 3023 3068 3074 3839 4436
Size: 393 626 259 506 45 6 765 597
Cuts at: 4436 5223 5491 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 765 626 597 506 393 268
259 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2084
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2991 2991
Size: 5776
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2107 4923 1678
Size: 429 2816 2531
Fragments arranged by size:
2816 2531 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2171 2203 2583
Size: 32 135 32 445 32 122 32 380
Cuts at: 2583 2615 4417 4449 4491 4523 1373
Size: 32 1802 32 42 32 2626
Fragments arranged by size:
2626 1802 445 380 135 122 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2617 3120 3366 669
Size: 988 281 679 503 246 3079
Fragments arranged by size:
3079 988 679 503 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2433 2440 2901 3192 3869 5017 5262 1606
Size: 827 7 461 291 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 677 461 291 245 7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2652 3134 4679 1132
Size: 97 1423 482 1545 2229
Fragments arranged by size:
2229 1545 1423 482 97
PasI CC'CwG_GG
Cuts at: 1348 2954 1348
Size: 1606 4170
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2640 3046 1108
Size: 972 560 406 3838
Fragments arranged by size:
3838 972 560 406
PfoI T'CCnGG_A
Cuts at: 3039 3039
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2991 2991
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2405 2835 2891 3128 830
Size: 1575 430 56 237 3478
Fragments arranged by size:
3478 1575 430 237 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2440 2440
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2401 2831 2887 3124 4259 4940 5131
Size: 219 1356 430 56 237 1135 681 191
Cuts at: 5131 826
Size: 1471
Fragments arranged by size:
1471 1356 1135 681 430 237 219 191
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2124 2161 2684 3103 1872
Size: 252 37 523 419 4545
Fragments arranged by size:
4545 523 419 252 37
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2608 3880 4781 5043 820
Size: 197 926 665 1272 901 262 1553
Fragments arranged by size:
1553 1272 926 901 665 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 2652 3134 1132
Size: 1520 482 3774
Fragments arranged by size:
3774 1520 482
SrfI GCCC'GGGC
Cuts at: 2124 2124
Size: 5776
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2553 3032 1517
Size: 1036 479 4261
Fragments arranged by size:
4261 1036 479
StyI C'CwwG_G
Cuts at: 513 1065 2587 3215 513
Size: 552 1522 628 3074
Fragments arranged by size:
3074 1522 628 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2998 3030 5736 5768 2998
Size: 32 2706 32 3006
Fragments arranged by size:
3006 2706 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3070 3070
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 2122 2159 2682 3101 1870
Size: 252 37 523 419 4545
Fragments arranged by size:
4545 523 419 252 37
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SwaI