Restriction map for
pFN21AB6487
(Circular) (Six-base) MAPSORT of: pFN21AB6487.seq Check: 6389 from: 1 to: 5692
pFN21AB6487 5692 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 3755 5139 5407 1632
Size: 918 1205 1384 268 1917
Fragments arranged by size:
1917 1384 1205 918 268
AflII C'TTAA_G
Cuts at: 820 1017 2582 820
Size: 197 1565 3930
Fragments arranged by size:
3930 1565 197
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2389 2491 3435 5007 2389
Size: 102 944 1572 3074
Fragments arranged by size:
3074 1572 944 102
ApaI G_GGCC'C
Cuts at: 2027 2828 2027
Size: 801 4891
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2762 3001 3227 3379 5488 1493
Size: 1269 239 226 152 2109 1697
Fragments arranged by size:
2109 1697 1269 239 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2068 2075 2472 2820 3017 1870
Size: 73 77 48 7 397 348 197 4545
Fragments arranged by size:
4545 397 348 197 77 73 48 7
BamHI G'GATC_C
Cuts at: 2925 3022 3389 2925
Size: 97 367 5228
Fragments arranged by size:
5228 367 97
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2937 3013 4468 618
Size: 325 217 627 1150 76 1455 1842
Fragments arranged by size:
1842 1455 1150 627 325 217 76
BanII G_rGCy'C
Cuts at: 1391 2027 2828 3011 1391
Size: 636 801 183 4072
Fragments arranged by size:
4072 801 636 183
BbeI G_GCGC'C
Cuts at: 2941 2941
Size: 5692
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2974 928
Size: 971 1075 3646
Fragments arranged by size:
3646 1075 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2039 4303
Size: 107 122 71 631 605 337 30 2264
Cuts at: 4303 136
Size: 1525
Fragments arranged by size:
2264 1525 631 605 337 122 107 71
30
BglII A'GATC_T
Cuts at: 2664 5687 2664
Size: 3023 2669
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2828 3753 4909 1790
Size: 237 801 925 1156 2573
Fragments arranged by size:
2573 1156 925 801 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2354 3423 4381
Size: 416 53 83 399 24 937 1069 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1069 958 937 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 2886 4352 1239
Size: 1625 22 1466 2579
Fragments arranged by size:
2579 1625 1466 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2087 2627 3817 4682 4980 2087
Size: 540 1190 865 298 2799
Fragments arranged by size:
2799 1190 865 540 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2105 2783 4355 882
Size: 617 579 27 678 1572 2219
Fragments arranged by size:
2219 1572 678 617 579 27
BsaAI yAC'GTr
Cuts at: 493 1204 2131 3483 493
Size: 711 927 1352 2702
Fragments arranged by size:
2702 1352 927 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2938 3499 3881 4516
Size: 53 83 186 936 1405 561 382 635
Cuts at: 4516 275
Size: 1451
Fragments arranged by size:
1451 1405 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2265 4125 4523 4797 4944 5355 2265
Size: 1860 398 274 147 411 2602
Fragments arranged by size:
2602 1860 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2238 2260 2268 2290 2420
Size: 30 165 30 835 22 8 22 130
Cuts at: 2420 2450 2534 2564 1178
Size: 30 84 30 4306
Fragments arranged by size:
4306 835 165 130 84 30 30 30
30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 2801 1186
Size: 1306 309 4077
Fragments arranged by size:
4077 1306 309
BseYI C'CCAG_C
Cuts at: 2356 2833 4895 5391 2356
Size: 477 2062 496 2657
Fragments arranged by size:
2657 2062 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2510 2701 1163
Size: 573 666 108 191 4154
Fragments arranged by size:
4154 666 573 191 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2221 2275 3142 3903 4931 1540
Size: 275 186 220 54 867 761 1028 2301
Fragments arranged by size:
2301 1028 867 761 275 220 186 54
BsiHKAI G_wGCw'C
Cuts at: 2913 3753 3838 4909 2913
Size: 840 85 1071 3696
Fragments arranged by size:
3696 1071 840 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2828 2913 3011 3753 3838 4909
Size: 399 237 801 85 98 742 85 1071
Cuts at: 4909 1391
Size: 2174
Fragments arranged by size:
2174 1071 801 742 399 237 98 85
85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 3047 844
Size: 1466 737 3489
Fragments arranged by size:
3489 1466 737
BsrBI CCG'CTC
Cuts at: 1394 1823 2271 3579 1394
Size: 429 448 1308 3507
Fragments arranged by size:
3507 1308 448 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 2512
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2664 2778 2925 3022 3389 3774 3791 5243
Size: 692 114 147 97 367 385 17 1452
Cuts at: 5243 5329 5341 5687 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 692 385 367 346 147 114
97 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2457 2511 2623 2884 513
Size: 552 972 420 54 112 261 3321
Fragments arranged by size:
3321 972 552 420 261 112 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2321 3208 4002 4022 2321
Size: 887 794 20 3991
Fragments arranged by size:
3991 887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2272 2493
Size: 54 1425 50 275 18 388 54 221
Cuts at: 2493 2881 2968 3139 4028 5657 8
Size: 388 87 171 889 1629 43
Fragments arranged by size:
1629 1425 889 388 388 275 221 171
87 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2218 2272 3139 1537
Size: 275 406 54 867 4090
Fragments arranged by size:
4090 867 406 275 54
EarI CTCTTCn'nnn_
Cuts at: 1353 2642 2892 3622 1353
Size: 1289 250 730 3423
Fragments arranged by size:
3423 1289 730 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4279 4653 4799 387
Size: 1064 138 438 2252 374 146 1280
Fragments arranged by size:
2252 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 2864 2886 3755 4352 5139 5407
Size: 393 918 314 22 869 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 918 869 787 597 393 314 268
22
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2393 2843 2381
Size: 12 450 5230
Fragments arranged by size:
5230 450 12
EcoO109I rG'GnC_Cy
Cuts at: 2386 2824 2386
Size: 438 5254
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 2174 4198 2174
Size: 2024 3668
HaeII r_GCGC'y
Cuts at: 1678 2414 2941 4839 1678
Size: 736 527 1898 2531
Fragments arranged by size:
2531 1898 736 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2260 2292 2420 2452 4333
Size: 32 135 32 688 32 128 32 1881
Cuts at: 4333 4365 4407 4439 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1881 688 135 128 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KasI G'GCGC_C
Cuts at: 2937 2937
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2495 2970 10
Size: 54 1425 343 663 475 2732
Fragments arranged by size:
2732 1425 663 475 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 3650 4009 4168 518
Size: 648 1290 1194 359 159 2042
Fragments arranged by size:
2042 1290 1194 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2110 2217 2407 2443 2864 2886 3108
Size: 433 71 107 190 36 421 22 222
Cuts at: 3108 3785 4933 5178 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 433 421 245 222 190
107 71 36 22
NarI GG'CG_CC
Cuts at: 2938 2938
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 2457 513
Size: 552 1392 3748
Fragments arranged by size:
3748 1392 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 2218 3139 2218
Size: 921 4771
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PfoI T'CCnGG_A
Cuts at: 2841 2841
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2958 1613
Size: 1345 4347
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2023 2824 2023
Size: 801 4891
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2111 2143 2111
Size: 32 5660
PstI C_TGCA'G
Cuts at: 830 2418 3044 830
Size: 1588 626 3478
Fragments arranged by size:
3478 1588 626
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2407 2864 2407
Size: 457 5235
SacII CC_GC'GG
Cuts at: 2040 2887 2040
Size: 847 4845
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 2549 3940 5652 2549
Size: 1391 1712 2589
Fragments arranged by size:
2589 1712 1391
SexAI A'CCwGG_T
Cuts at: 2376 2376
Size: 5692
SfcI C'TryA_G
Cuts at: 826 1045 2414 3040 4175 4856 5047 826
Size: 219 1369 626 1135 681 191 1471
Fragments arranged by size:
1471 1369 1135 681 626 219 191
SfoI GGC'GCC
Cuts at: 2939 2939
Size: 5692
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 2474 2822 3019 1872
Size: 602 348 197 4545
Fragments arranged by size:
4545 602 348 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2102 2582 2642 3796 4697
Size: 197 926 132 27 480 60 1154 901
Cuts at: 4697 4959 820
Size: 262 1553
Fragments arranged by size:
1553 1154 926 901 480 262 197 132
60 27
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2018 1517
Size: 501 5191
StyI C'CwwG_G
Cuts at: 513 1065 2457 2754 3131 513
Size: 552 1392 297 377 3074
Fragments arranged by size:
3074 1392 552 377 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2242 2252 2963 3719 3736 3889 4074
Size: 1255 662 10 711 756 17 153 185
Cuts at: 4074 4519 325
Size: 445 1498
Fragments arranged by size:
1498 1255 756 711 662 445 185 153
17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 3938 5650
Size: 274 80 33 51 1443 570 1391 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1443 1391 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 5652 5684 2238
Size: 32 3382 32 2246
Fragments arranged by size:
3382 2246 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2434 1316
Size: 1118 4574
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 2075 1943
Size: 132 5560
XmaI C'CCGG_G
Cuts at: 1870 2472 2820 3017 1870
Size: 602 348 197 4545
Fragments arranged by size:
4545 602 348 197
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI RsrII SacI SanDI SapI
SfiI SrfI SwaI XcmI