Restriction map for
pFN21AB6486
(Circular) (Six-base) MAPSORT of: pFN21AB6486.seq Check: 6610 from: 1 to: 5773
pFN21AB6486 5773 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2596 2596
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 3836 5220 5488 1632
Size: 1073 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 1073 268
AflII C'TTAA_G
Cuts at: 820 1017 2717 820
Size: 197 1700 3876
Fragments arranged by size:
3876 1700 197
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 3019 3022 3516 5088 3019
Size: 3 494 1572 3704
Fragments arranged by size:
3704 1572 494 3
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 2017 2884 3082 3308 3460 5569 1493
Size: 524 867 198 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 524 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 3098 1870
Size: 73 1155 4545
Fragments arranged by size:
4545 1155 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 3094 4549 618
Size: 325 217 627 298 1009 1455 1842
Fragments arranged by size:
1842 1455 1009 627 325 298 217
BanII G_rGCy'C
Cuts at: 1391 2982 3092 1391
Size: 1591 110 4072
Fragments arranged by size:
4072 1591 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4802
BbvCI CC'TCA_GC
Cuts at: 2442 3036 2442
Size: 594 5179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 2835 1548
Size: 1287 4486
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2496 3834 4990 1790
Size: 706 1338 1156 2573
Fragments arranged by size:
2573 1338 1156 706
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2566 4433 1239
Size: 820 507 1867 2579
Fragments arranged by size:
2579 1867 820 507
Bpu10I CC'TnA_GC
Cuts at: 2433 2442 2976 3036 2433
Size: 9 534 60 5170
Fragments arranged by size:
5170 534 60 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 4436 882
Size: 617 636 2301 2219
Fragments arranged by size:
2301 2219 636 617
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 3962 4597 275
Size: 53 83 186 936 2047 382 635 1451
Fragments arranged by size:
2047 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5548
Fragments arranged by size:
5548 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5773
BseYI C'CCAG_C
Cuts at: 2447 2762 4976 5472 2447
Size: 315 2214 496 2748
Fragments arranged by size:
2748 2214 496 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2982 3834 3919 4990 2982
Size: 852 85 1071 3765
Fragments arranged by size:
3765 1071 852 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
BsmBI CGTCTCn'nnnn_
Cuts at: 2032 2032
Size: 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2496 2982 3092 3834 3919 4990 1391
Size: 399 706 486 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 486 399 110 85
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3004 3662 5392 3004
Size: 658 1730 3385
Fragments arranged by size:
3385 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2578 2596 3128 844
Size: 1371 363 18 532 3489
Fragments arranged by size:
3489 1371 532 363 18
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 4417 4604 1700
Size: 110 272 2335 187 2869
Fragments arranged by size:
2869 2335 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 2821 96
Size: 2725 3048
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3022 794
Size: 2228 3545
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2663 513
Size: 552 1598 3623
Fragments arranged by size:
3623 1598 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 3289 4083 4103 3289
Size: 794 20 4959
Fragments arranged by size:
4959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2230 2265 529
Size: 35 1666 35 4037
Fragments arranged by size:
4037 1666 35 35
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DraIII CAC_nnn'GTG
Cuts at: 2314 2734 2314
Size: 420 5353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2296 2479 2694
Size: 54 1425 50 275 18 466 183 215
Cuts at: 2694 3220 4109 5738 8
Size: 526 889 1629 43
Fragments arranged by size:
1629 1425 889 526 466 275 215 183
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 2381 3703 1353
Size: 993 35 1322 3423
Fragments arranged by size:
3423 1322 993 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4360 4734 4880 387
Size: 1064 138 2771 374 146 1280
Fragments arranged by size:
2771 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2980 2980
Size: 5773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2566 2705 3836 4433 5220 5488
Size: 393 427 507 139 1131 597 787 268
Cuts at: 5488 1239
Size: 1524
Fragments arranged by size:
1524 1131 787 597 507 427 393 268
139
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 5773
EcoO109I rG'GnC_Cy
Cuts at: 2575 2575
Size: 5773
EcoRI G'AATT_C
Cuts at: 2884 3082 3460 2884
Size: 198 378 5197
Fragments arranged by size:
5197 378 198
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 4920 1678
Size: 3242 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2943 2975 4414 4446 4488
Size: 32 135 32 1371 32 1439 32 42
Cuts at: 4488 4520 1373
Size: 32 2626
Fragments arranged by size:
2626 1439 1371 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 4887 5071 1305
Size: 750 2832 184 2007
Fragments arranged by size:
2832 2007 750 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2298 2481 2696 10
Size: 54 1425 343 466 183 215 3087
Fragments arranged by size:
3087 1425 466 343 215 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2829 3731 4090 4249 518
Size: 648 1663 902 359 159 2042
Fragments arranged by size:
2042 1663 902 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3019 3040 3189 3866 5014 5259 1606
Size: 1413 21 149 677 1148 245 2120
Fragments arranged by size:
2120 1413 1148 677 245 149 21
NaeI GCC'GGC
Cuts at: 2084 2084
Size: 5773
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5221
NdeI CA'TA_TG
Cuts at: 387 2724 387
Size: 2337 3436
NgoMIV G'CCGG_C
Cuts at: 2082 2082
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2536 1348
Size: 1188 4585
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2653 1108
Size: 1545 4228
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 2575
Size: 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PstI C_TGCA'G
Cuts at: 830 2292 3125 830
Size: 1462 833 3478
Fragments arranged by size:
3478 1462 833
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 3019 3040 3019
Size: 21 5752
SacI G_AGCT'C
Cuts at: 2982 2982
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2288 2415 3121 4256 4937 5128 826
Size: 219 1243 127 706 1135 681 191 1471
Fragments arranged by size:
1471 1243 1135 706 681 219 191 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3100 1872
Size: 1228 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3877 4778 5040 820
Size: 197 926 774 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 774 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 3212 513
Size: 552 2147 3074
Fragments arranged by size:
3074 2147 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2179 2821 4019
Size: 274 80 33 51 1443 202 642 1198
Cuts at: 4019 5731 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1198 642 274 202 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5733 5765 5733
Size: 32 5741
Tth111I GACn'n_nGTC
Cuts at: 1316 2255 1316
Size: 939 4834
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3098 1870
Size: 1228 4545
XmnI GAAnn'nnTTC
Cuts at: 2888 3902 2888
Size: 1014 4759
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI