Restriction map for
pFN21AB6485
(Circular) (Six-base) MAPSORT of: pFN21AB6485.seq Check: 1830 from: 1 to: 5689
pFN21AB6485 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2569 2569
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 3032 1937
Size: 994 101 4594
Fragments arranged by size:
4594 994 101
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2438 2577 2726 3752 5136 5404 1632
Size: 617 189 139 149 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 617 268 189 149 139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2633 1166
Size: 1467 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2539 2600 3432 5004 2539
Size: 61 832 1572 3224
Fragments arranged by size:
3224 1572 832 61
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2697 2718 2760 3014 1870
Size: 73 754 21 42 254 4545
Fragments arranged by size:
4545 754 254 73 42 21
BamHI G'GATC_C
Cuts at: 2502 3019 3386 2502
Size: 517 367 4805
Fragments arranged by size:
4805 517 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2559 3008 1391
Size: 1168 449 4072
Fragments arranged by size:
4072 1168 449
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 2714 928
Size: 971 338 477 3903
Fragments arranged by size:
3903 971 477 338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 2980 1548
Size: 1432 4257
BfrBI ATG'CAT
Cuts at: 2519 2519
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2820 3750 4906 1790
Size: 243 787 930 1156 2573
Fragments arranged by size:
2573 1156 930 787 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 2429 2827 4349 1239
Size: 1083 107 398 1522 2579
Fragments arranged by size:
2579 1522 1083 398 107
Bpu10I CC'TnA_GC
Cuts at: 2015 2015
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2297 4352 882
Size: 617 798 2055 2219
Fragments arranged by size:
2219 2055 798 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2496 4122 4520 4794 4941 5352 2496
Size: 1626 398 274 147 411 2833
Fragments arranged by size:
2833 1626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2432 2489 1186
Size: 874 372 57 4386
Fragments arranged by size:
4386 874 372 57
BseYI C'CCAG_C
Cuts at: 2574 2819 4892 5388 2574
Size: 245 2073 496 2875
Fragments arranged by size:
2875 2073 496 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3139 3900 4928 1540
Size: 275 186 871 267 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 267 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2559 3750 3835 4906 2016
Size: 543 1191 85 1071 2799
Fragments arranged by size:
2799 1191 1071 543 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2033 2559 2820 3008 3750 3835
Size: 399 226 17 526 261 188 742 85
Cuts at: 3835 4906 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 261 226 188
85 17
BspEI T'CCGG_A
Cuts at: 2496 5352 2496
Size: 2856 2833
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 2797 3044 844
Size: 1725 228 247 3489
Fragments arranged by size:
3489 1725 247 228
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2871 4333 4520 1700
Size: 110 1061 1462 187 2869
Fragments arranged by size:
2869 1462 1061 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2743 1184
Size: 1559 4130
BstYI r'GATC_y
Cuts at: 1972 2502 3019 3386 3771 3788 5240 5326 5338
Size: 530 517 367 385 17 1452 86 12
Cuts at: 5338 5684 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 530 517 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2162 2162
Size: 5689
BtgI C'CryG_G
Cuts at: 513 1065 2004 2380 513
Size: 552 939 376 3822
Fragments arranged by size:
3822 939 552 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2238 2628 2742 3205 3999 4019 2238
Size: 390 114 463 794 20 3908
Fragments arranged by size:
3908 794 463 390 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2242 2277 529
Size: 35 1678 35 3941
Fragments arranged by size:
3941 1678 35 35
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2869 2889 3136
Size: 54 1425 50 275 18 1039 20 247
Cuts at: 3136 4025 5654 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 275 247 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 3136 1537
Size: 275 1057 267 4090
Fragments arranged by size:
4090 1057 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 3619 1353
Size: 756 1510 3423
Fragments arranged by size:
3423 1510 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2322 2429 2438 2577 2726 2827
Size: 393 617 73 107 9 139 149 101
Cuts at: 2827 3752 4349 5136 5404 1239
Size: 925 597 787 268 1524
Fragments arranged by size:
1524 925 787 617 597 393 268 149
139 107 101 73 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2114 2042
Size: 72 5617
EcoO109I rG'GnC_Cy
Cuts at: 2149 2722 2940 2949 2149
Size: 573 218 9 4889
Fragments arranged by size:
4889 573 218 9
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2211 4836 1678
Size: 533 2625 2531
Fragments arranged by size:
2625 2531 533
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373
Size: 32 135 32 2758 32 42 32 2626
Fragments arranged by size:
2758 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2002 4803 4987 1305
Size: 697 2801 184 2007
Fragments arranged by size:
2801 2007 697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2891 10
Size: 54 1425 343 1059 2808
Fragments arranged by size:
2808 1425 1059 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2633 2735 3647 4006 4165 518
Size: 648 843 624 102 912 359 159 2042
Fragments arranged by size:
2042 912 843 648 624 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2449 2692 2775 3105 3782 4930 5175 1606
Size: 843 243 83 330 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 330 245 243 83
NaeI GCC'GGC
Cuts at: 2873 2873
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 2004 2380 513
Size: 552 939 376 3822
Fragments arranged by size:
3822 939 552 376
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2871 2871
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NsiI A_TGCA'T
Cuts at: 2521 2521
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2519 3047 4592 1132
Size: 97 1290 528 1545 2229
Fragments arranged by size:
2229 1545 1290 528 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 2926 1108
Size: 1431 387 3871
Fragments arranged by size:
3871 1431 387
PfoI T'CCnGG_A
Cuts at: 2804 2835 2919 2804
Size: 31 84 5574
Fragments arranged by size:
5574 84 31
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2722 2940 2949 2722
Size: 218 9 5462
Fragments arranged by size:
5462 218 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2107 2391 2553 3041 830
Size: 1277 284 162 488 3478
Fragments arranged by size:
3478 1277 488 284 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2449 2692 2449
Size: 243 5446
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SexAI A'CCwGG_T
Cuts at: 2094 2094
Size: 5689
SfcI C'TryA_G
Cuts at: 826 1045 2103 2387 2549 3037 4172 4853 5044
Size: 219 1058 284 162 488 1135 681 191
Cuts at: 5044 826
Size: 1471
Fragments arranged by size:
1471 1135 1058 681 488 284 219 191
162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2720 3016 1872
Size: 848 296 4545
Fragments arranged by size:
4545 848 296
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2760 3793 4694 4956 820
Size: 197 926 817 1033 901 262 1553
Fragments arranged by size:
1553 1033 926 901 817 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 2519 3047 1132
Size: 1387 528 3774
Fragments arranged by size:
3774 1387 528
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2380 2725 3128 513
Size: 552 939 376 345 403 3074
Fragments arranged by size:
3074 939 552 403 376 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2614 3716 3733 3886 4071 4516 325
Size: 1255 1034 1102 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1102 1034 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 2624 1316
Size: 1308 4381
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2582 2582
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 2760 1943
Size: 817 4872
XmaI C'CCGG_G
Cuts at: 1870 2718 3014 1870
Size: 848 296 4545
Fragments arranged by size:
4545 848 296
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NarI NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI