Restriction map for
pFN21AB6482
(Circular) (Six-base) MAPSORT of: pFN21AB6482.seq Check: 4084 from: 1 to: 5773
pFN21AB6482 5773 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 3836 5220 5488 1632
Size: 797 1407 1384 268 1917
Fragments arranged by size:
1917 1407 1384 797 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 2427 1166
Size: 1261 4512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2864 1184
Size: 1680 4093
AlwNI CAG_nnn'CTG
Cuts at: 3516 5088 3516
Size: 1572 4201
ApaI G_GGCC'C
Cuts at: 2129 2501 2129
Size: 372 5401
ApaLI G'TGCA_C
Cuts at: 2886 3830 4986 2886
Size: 944 1156 3673
Fragments arranged by size:
3673 1156 944
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 2906 3098 1870
Size: 73 185 778 192 4545
Fragments arranged by size:
4545 778 192 185 73
AvrII C'CTAG_G
Cuts at: 2281 2281
Size: 5773
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 2460 3094 4549 618
Size: 325 217 627 567 106 634 1455 1842
Fragments arranged by size:
1842 1455 634 627 567 325 217 106
BanII G_rGCy'C
Cuts at: 1391 2072 2129 2146 2501 2718 2735 2899 2907
Size: 681 57 17 355 217 17 164 8
Cuts at: 2907 3092 1391
Size: 185 4072
Fragments arranged by size:
4072 681 355 217 185 164 57 17
17 8
BbeI G_GCGC'C
Cuts at: 2358 2464 2358
Size: 106 5667
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 928
Size: 971 247 4555
Fragments arranged by size:
4555 971 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2818 2852 3964 3998 1903
Size: 34 881 34 1112 34 3678
Fragments arranged by size:
3678 1112 881 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2422 2434 2440 2501 2890 3834 4990
Size: 339 293 12 6 61 389 944 1156
Cuts at: 4990 1790
Size: 2573
Fragments arranged by size:
2573 1156 944 389 339 293 61 12
6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 2712 2795 4433 1239
Size: 1385 88 83 1638 2579
Fragments arranged by size:
2579 1638 1385 88 83
Bpu10I CC'TnA_GC
Cuts at: 2493 2901 2493
Size: 408 5365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 882
Size: 617 2937 2219
Fragments arranged by size:
2937 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2641 2721 3564 493
Size: 711 1437 80 843 2702
Fragments arranged by size:
2702 1437 843 711 80
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2355 2461 3580 3962
Size: 53 83 186 936 822 106 1119 382
Cuts at: 3962 4597 275
Size: 635 1451
Fragments arranged by size:
1451 1119 936 822 635 382 186 106
83 53
BsaWI w'CCGG_w
Cuts at: 2080 2710 4206 4604 4878 5025 5436 2080
Size: 630 1496 398 274 147 411 2417
Fragments arranged by size:
2417 1496 630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 2705 2735 1178
Size: 30 165 30 924 30 348 30 4216
Fragments arranged by size:
4216 924 348 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 2597 2870 1186
Size: 1402 9 273 4089
Fragments arranged by size:
4089 1402 273 9
BseYI C'CCAG_C
Cuts at: 2009 3034 4976 5472 2009
Size: 1025 1942 496 2310
Fragments arranged by size:
2310 1942 1025 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2282 2381 1163
Size: 573 438 108 99 4555
Fragments arranged by size:
4555 573 438 108 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2250 2728 3223 3984 5012 1540
Size: 275 186 249 478 495 761 1028 2301
Fragments arranged by size:
2301 1028 761 495 478 275 249 186
BsiHKAI G_wGCw'C
Cuts at: 2890 3834 3919 4990 2890
Size: 944 85 1071 3673
Fragments arranged by size:
3673 1071 944 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
BsmBI CGTCTCn'nnnn_
Cuts at: 2958 2958
Size: 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2129 2146 2422 2434 2440 2501
Size: 399 282 57 17 276 12 6 61
Cuts at: 2501 2718 2735 2890 2899 2907 3092 3834 3919
Size: 217 17 155 9 8 185 742 85
Cuts at: 3919 4990 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 282 276 217 185
155 85 61 57 17 17 12 9
8 6
BspEI T'CCGG_A
Cuts at: 2080 5436 2080
Size: 3356 2417
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 3128 844
Size: 1433 851 3489
Fragments arranged by size:
3489 1433 851
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2249 4417 4604 1700
Size: 110 439 2168 187 2869
Fragments arranged by size:
2869 2168 439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2786 2786
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 3069 513
Size: 552 939 324 741 3217
Fragments arranged by size:
3217 939 741 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2988 487
Size: 683 56 1762 3272
Fragments arranged by size:
3272 1762 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2754 2848 3289 4083 4103 2186
Size: 568 94 441 794 20 3856
Fragments arranged by size:
3856 794 568 441 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 2643 2725
Size: 54 1425 50 275 18 417 396 82
Cuts at: 2725 2824 3220 4109 5738 8
Size: 99 396 889 1629 43
Fragments arranged by size:
1629 1425 889 417 396 396 275 99
82 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2247 2725 3220 1537
Size: 275 435 478 495 4090
Fragments arranged by size:
4090 495 478 435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 3054 3703 1353
Size: 1212 489 649 3423
Fragments arranged by size:
3423 1212 649 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2387 4360 4734 4880 387
Size: 1064 138 798 1973 374 146 1280
Fragments arranged by size:
1973 1280 1064 798 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2624 2712 2795 3836 4433 5220
Size: 393 797 195 88 83 1041 597 787
Cuts at: 5220 5488 1239
Size: 268 1524
Fragments arranged by size:
1524 1041 797 787 597 393 268 195
88 83
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 2492
Size: 5773
EcoO109I rG'GnC_Cy
Cuts at: 2102 2497 2498 2102
Size: 395 1 5377
Fragments arranged by size:
5377 395 1
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 2358 2396 2464 4920 1678
Size: 680 38 68 2456 2531
Fragments arranged by size:
2531 2456 680 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 4414 4446 4488
Size: 32 135 32 1337 32 1473 32 42
Cuts at: 4488 4520 1373
Size: 32 2626
Fragments arranged by size:
2626 1473 1337 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KasI G'GCGC_C
Cuts at: 2354 2460 2354
Size: 106 5667
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 4887 5071 1305
Size: 1272 2310 184 2007
Fragments arranged by size:
2310 2007 1272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3951
Fragments arranged by size:
3951 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2427 2658 3731 4090 4249 518
Size: 648 1045 216 231 1073 359 159 2042
Fragments arranged by size:
2042 1073 1045 648 359 231 216 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2090 2602 2961 3189 3866 5014 5259
Size: 455 29 512 359 228 677 1148 245
Cuts at: 5259 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 512 455 359 245 228
29
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 5773
NarI GG'CG_CC
Cuts at: 2355 2461 2355
Size: 106 5667
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4282
Fragments arranged by size:
4282 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 2818 3131 4676 1132
Size: 97 1589 313 1545 2229
Fragments arranged by size:
2229 1589 1545 313 97
PasI CC'CwG_GG
Cuts at: 1348 2074 2466 1348
Size: 726 392 4655
Fragments arranged by size:
4655 726 392
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PmlI CAC'GTG
Cuts at: 2641 2721 2641
Size: 80 5693
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2102 2102
Size: 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2125 2497 2125
Size: 372 5401
PstI C_TGCA'G
Cuts at: 830 2274 2601 2939 3125 830
Size: 1444 327 338 186 3478
Fragments arranged by size:
3478 1444 338 327 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2602 2602
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2270 2597 2935 3121 4256 4937 5128
Size: 219 1225 327 338 186 1135 681 191
Cuts at: 5128 826
Size: 1471
Fragments arranged by size:
1471 1225 1135 681 338 327 219 191
186
SfoI GGC'GCC
Cuts at: 2356 2462 2356
Size: 106 5667
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 2130 3100 1872
Size: 258 970 4545
Fragments arranged by size:
4545 970 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3877 4778 5040 820
Size: 197 926 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2004 2281 3212 513
Size: 552 939 277 931 3074
Fragments arranged by size:
3074 939 931 552 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4019 5731
Size: 274 80 33 51 1443 282 1760 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1760 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2705 2737 5733 5765 2705
Size: 32 2996 32 2713
Fragments arranged by size:
2996 2713 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 2128 3098 1870
Size: 258 970 4545
Fragments arranged by size:
4545 970 258
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI