Restriction map for
pFN21AB6478
(Circular) (Six-base) MAPSORT of: pFN21AB6478.seq Check: 2970 from: 1 to: 5770
pFN21AB6478 5770 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2179 2501 3833 5217 5485 1632
Size: 547 322 1332 1384 268 1917
Fragments arranged by size:
1917 1384 1332 547 322 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 2409 4669 5646 1203
Size: 1206 2260 977 1327
Fragments arranged by size:
2260 1327 1206 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 2157 2462 1166
Size: 991 305 4474
Fragments arranged by size:
4474 991 305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2468 3091 4546 618
Size: 325 217 627 372 309 623 1455 1842
Fragments arranged by size:
1842 1455 627 623 372 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2358 2400 2901 2961 2988 3089 1391
Size: 967 42 501 60 27 101 4072
Fragments arranged by size:
4072 967 501 101 60 42 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2127 928
Size: 971 228 4571
Fragments arranged by size:
4571 971 228
BbvCI CC'TCA_GC
Cuts at: 2546 2546
Size: 5770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2732 2766 3961 3995 1903
Size: 34 795 34 1195 34 3678
Fragments arranged by size:
3678 1195 795 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2609 2703 4381
Size: 107 122 71 631 605 937 94 1678
Cuts at: 4381 136
Size: 1525
Fragments arranged by size:
1678 1525 937 631 605 122 107 94
71
BglII A'GATC_T
Cuts at: 2070 5765 2070
Size: 3695 2075
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2471 3831 4987 1790
Size: 681 1360 1156 2573
Fragments arranged by size:
2573 1360 1156 681
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 3501 4459
Size: 416 53 83 399 24 1158 926 958
Cuts at: 4459 442
Size: 1753
Fragments arranged by size:
1753 1158 958 926 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2327 2837 2870 2915 4430 1239
Size: 875 213 510 33 45 1515 2579
Fragments arranged by size:
2579 1515 875 510 213 45 33
Bpu10I CC'TnA_GC
Cuts at: 2368 2511 2546 2368
Size: 143 35 5592
Fragments arranged by size:
5592 143 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2240 2312 1186
Size: 916 138 72 4644
Fragments arranged by size:
4644 916 138 72
BseYI C'CCAG_C
Cuts at: 2641 2666 2967 3065 4973 5469 2641
Size: 25 301 98 1908 496 2942
Fragments arranged by size:
2942 1908 496 301 98 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2485 1163
Size: 573 585 164 4448
Fragments arranged by size:
4448 585 573 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2587 2746 3220 3981 5009 1540
Size: 275 186 586 159 474 761 1028 2301
Fragments arranged by size:
2301 1028 761 586 474 275 186 159
BsiHKAI G_wGCw'C
Cuts at: 2400 3831 3916 4987 2400
Size: 1431 85 1071 3183
Fragments arranged by size:
3183 1431 1071 85
BsiWI C'GTAC_G
Cuts at: 2037 2037
Size: 5770
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2358 2400 2471 2901 2961 2988 3089
Size: 399 568 42 71 430 60 27 101
Cuts at: 3089 3831 3916 4987 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 430 399 101 85
71 60 42 27
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 2787 2799 3659 5389 2787
Size: 12 860 1730 3168
Fragments arranged by size:
3168 1730 860 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 2733 3125 844
Size: 1841 48 392 3489
Fragments arranged by size:
3489 1841 392 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 3007 3657 1394
Size: 429 275 909 650 3507
Fragments arranged by size:
3507 909 650 429 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2162 2493 2942 4414 4601 1700
Size: 110 352 331 449 1472 187 2869
Fragments arranged by size:
2869 1472 449 352 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 2268 1925
Size: 343 5427
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 2641
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2070 2658 2820 3100 3467 3852 3869 5321
Size: 98 588 162 280 367 385 17 1452
Cuts at: 5321 5407 5419 5765 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 588 385 367 346 280 162
98 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2206 2220 2401 2472 2487 2505 2767
Size: 552 1141 14 181 71 15 18 262
Cuts at: 2767 513
Size: 3516
Fragments arranged by size:
3516 1141 552 262 181 71 18 15
14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2634 487
Size: 683 56 1408 3623
Fragments arranged by size:
3623 1408 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 3286 4080 4100 2172
Size: 1114 794 20 3842
Fragments arranged by size:
3842 1114 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735
Size: 54 1425 50 275 18 1387 889 1629
Cuts at: 5735 8
Size: 43
Fragments arranged by size:
1629 1425 1387 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3700 1353
Size: 2347 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2398 2398
Size: 5770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2179 2327 2501 2837 2870 2915
Size: 393 482 65 148 174 336 33 45
Cuts at: 2915 3833 4430 5217 5485 1239
Size: 918 597 787 268 1524
Fragments arranged by size:
1524 918 787 597 482 393 336 268
174 148 65 45 33
EcoO109I rG'GnC_Cy
Cuts at: 2201 2590 2201
Size: 389 5381
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 2297 2698 2861 4917 1678
Size: 619 401 163 2056 2531
Fragments arranged by size:
2531 2056 619 401 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2445 2477 4411 4443 4485
Size: 32 135 32 873 32 1934 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 1934 873 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 2779 3114 3360 669
Size: 988 281 784 57 335 246 3079
Fragments arranged by size:
3079 988 784 335 281 246 57
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2157 2414 2462 3728 4087 4246
Size: 648 843 148 257 48 1266 359 159
Cuts at: 4246 518
Size: 2042
Fragments arranged by size:
2042 1266 843 648 359 257 159 148
48
MspA1I CmG'CkG
Cuts at: 1606 2305 2670 2683 3186 3863 5011 5256 1606
Size: 699 365 13 503 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 503 365 245 13
NaeI GCC'GGC
Cuts at: 2164 2495 2944 2164
Size: 331 449 4990
Fragments arranged by size:
4990 449 331
NcoI C'CATG_G
Cuts at: 513 1065 2220 2472 2767 513
Size: 552 1155 252 295 3516
Fragments arranged by size:
3516 1155 552 295 252
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2162 2493 2942 2162
Size: 331 449 4990
Fragments arranged by size:
4990 449 331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2413 3128 4673 1132
Size: 97 1184 715 1545 2229
Fragments arranged by size:
2229 1545 1184 715 97
PasI CC'CwG_GG
Cuts at: 1348 2379 1348
Size: 1031 4739
PciI A'CATG_T
Cuts at: 2409 4669 2409
Size: 2260 3510
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 1108
Size: 1239 4531
PfoI T'CCnGG_A
Cuts at: 2064 2064
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2201 2590 2201
Size: 389 5381
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2537 2658 2730 3122 830
Size: 1707 121 72 392 3478
Fragments arranged by size:
3478 1707 392 121 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2305 2305
Size: 5770
SacI G_AGCT'C
Cuts at: 2400 2400
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SanDI GG'GwC_CC
Cuts at: 2201 2201
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 2537 3122 2537
Size: 585 5185
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 2533 2654 2726 3118 4253 4934 5125
Size: 219 1488 121 72 392 1135 681 191
Cuts at: 5125 826
Size: 1471
Fragments arranged by size:
1488 1471 1135 681 392 219 191 121
72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2220 2472 2767 2901 3054 3209 513
Size: 552 1155 252 295 134 153 155 3074
Fragments arranged by size:
3074 1155 552 295 252 155 153 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4016 5728 96
Size: 274 80 33 51 1443 2039 1712 138
Fragments arranged by size:
2039 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 2459 2783 1316
Size: 1143 324 4303
Fragments arranged by size:
4303 1143 324
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2890 2386
Size: 504 5266
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI