Restriction map for
pFN21AB6476
(Circular) (Six-base) MAPSORT of: pFN21AB6476.seq Check: 9155 from: 1 to: 5770
pFN21AB6476 5770 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2257 2257
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 2186 2441 3833 5217 5485 1632
Size: 489 65 255 1392 1384 268 1917
Fragments arranged by size:
1917 1392 1384 489 268 255 65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 2734 3513 5085 2734
Size: 779 1572 3419
Fragments arranged by size:
3419 1572 779
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2123 2175 2594 3079 3305 3457 5566 1493
Size: 630 52 419 485 226 152 2109 1697
Fragments arranged by size:
2109 1697 630 485 419 226 152 52
AseI AT'TA_AT
Cuts at: 160 1032 2427 3036 4324 160
Size: 872 1395 609 1288 1606
Fragments arranged by size:
1606 1395 1288 872 609
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2339 3095 1870
Size: 73 396 756 4545
Fragments arranged by size:
4545 756 396 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 3091 4546 618
Size: 325 217 627 262 1042 1455 1842
Fragments arranged by size:
1842 1455 1042 627 325 262 217
BanII G_rGCy'C
Cuts at: 1391 2340 2829 3089 1391
Size: 949 489 260 4072
Fragments arranged by size:
4072 949 489 260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 2352 2864 928
Size: 971 380 73 512 3834
Fragments arranged by size:
3834 971 512 380 73
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 5770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2276 3662 4872 2276
Size: 1386 1210 3174
Fragments arranged by size:
3174 1386 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BfrBI ATG'CAT
Cuts at: 2366 2366
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 2226 3187 2226
Size: 961 4809
Bme1580I G_kGCm'C
Cuts at: 1790 3831 4987 1790
Size: 2041 1156 2573
Fragments arranged by size:
2573 2041 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4430 1239
Size: 3191 2579
Bpu10I CC'TnA_GC
Cuts at: 2403 2403
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2909 4433 882
Size: 617 1410 1524 2219
Fragments arranged by size:
2219 1524 1410 617
BsaAI yAC'GTr
Cuts at: 493 1204 3043 3561 493
Size: 711 1839 518 2702
Fragments arranged by size:
2702 1839 711 518
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2806 2836 1178
Size: 30 165 30 1403 30 4112
Fragments arranged by size:
4112 1403 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5770
BseYI C'CCAG_C
Cuts at: 2021 2305 4973 5469 2021
Size: 284 2668 496 2322
Fragments arranged by size:
2668 2322 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2829 3831 3916 4987 2340
Size: 489 1002 85 1071 3123
Fragments arranged by size:
3123 1071 1002 489 85
BsmI GAATG_Cn'
Cuts at: 2241 3281 3374 2241
Size: 1040 93 4637
Fragments arranged by size:
4637 1040 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2829 3089 3831 3916 4987 1391
Size: 399 550 489 260 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 489 399 260 85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2257 3125 844
Size: 1413 868 3489
Fragments arranged by size:
3489 1413 868
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2368 4265 4439 65
Size: 1183 1120 1897 174 1396
Fragments arranged by size:
1897 1396 1183 1120 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2734 794
Size: 1940 3830
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2256 3100 3467 3852 3869 5321 5407 5419
Size: 284 844 367 385 17 1452 86 12
Cuts at: 5419 5765 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 844 385 367 346 284 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3066 3066
Size: 5770
BtgI C'CryG_G
Cuts at: 513 1065 2004 2880 513
Size: 552 939 876 3403
Fragments arranged by size:
3403 939 876 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 3286 4080 4100 3286
Size: 794 20 4956
Fragments arranged by size:
4956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 2481 2947 3074 2481
Size: 466 127 5177
Fragments arranged by size:
5177 466 127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2573 4777 809
Size: 507 1257 2204 1802
Fragments arranged by size:
2204 1802 1257 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735
Size: 54 1425 50 275 18 1387 889 1629
Cuts at: 5735 8
Size: 43
Fragments arranged by size:
1629 1425 1387 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2595 3700 1353
Size: 1242 1105 3423
Fragments arranged by size:
3423 1242 1105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2338 2827 2338
Size: 489 5281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2186 2441 3833 4430 5217 5485
Size: 393 489 65 255 1392 597 787 268
Cuts at: 5485 1239
Size: 1524
Fragments arranged by size:
1524 1392 787 597 489 393 268 255
65
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2435 2435
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2479 2511 4411 4443 4485
Size: 32 135 32 907 32 1900 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 1900 907 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3728 4087 4246 518
Size: 648 1713 849 359 159 2042
Fragments arranged by size:
2042 1713 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2765 3186 3863 5011 5256 1606
Size: 1159 421 677 1148 245 2120
Fragments arranged by size:
2120 1159 1148 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2880 513
Size: 552 939 876 3403
Fragments arranged by size:
3403 939 876 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NsiI A_TGCA'T
Cuts at: 2368 2368
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2632 3128 4673 1132
Size: 97 1403 496 1545 2229
Fragments arranged by size:
2229 1545 1403 496 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 2538 3340 2538
Size: 802 4968
PstI C_TGCA'G
Cuts at: 830 2730 2739 3122 830
Size: 1900 9 383 3478
Fragments arranged by size:
3478 1900 383 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2765 2765
Size: 5770
SacI G_AGCT'C
Cuts at: 2340 2829 2340
Size: 489 5281
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 2726 2735 2747 2850 3118 4253 4934
Size: 219 1681 9 12 103 268 1135 681
Cuts at: 4934 5125 826
Size: 191 1471
Fragments arranged by size:
1681 1471 1135 681 268 219 191 103
12 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 2803 1517
Size: 1286 4484
StyI C'CwwG_G
Cuts at: 513 1065 2004 2798 2880 2929 3209 513
Size: 552 939 794 82 49 280 3074
Fragments arranged by size:
3074 939 794 552 280 82 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4016 5728 96
Size: 274 80 33 51 1443 2039 1712 138
Fragments arranged by size:
2039 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 2806 2838 5730 5762 2696
Size: 32 78 32 2892 32 2704
Fragments arranged by size:
2892 2704 78 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2395 3040 2395
Size: 645 5125
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 2199 3899 2199
Size: 1700 4070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI