Restriction map for
pFN21AB6470
(Circular) (Six-base) MAPSORT of: pFN21AB6470.seq Check: 6824 from: 1 to: 5767
pFN21AB6470 5767 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 2635 3088 2635
Size: 453 5314
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2059 3830 5214 5482 1632
Size: 427 1771 1384 268 1917
Fragments arranged by size:
1917 1771 1384 427 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2754 4666 5643 1203
Size: 1551 1912 977 1327
Fragments arranged by size:
1912 1551 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2701 3510 5082 2701
Size: 809 1572 3386
Fragments arranged by size:
3386 1572 809
ApaI G_GGCC'C
Cuts at: 2234 2281 2291 2234
Size: 47 10 5710
Fragments arranged by size:
5710 47 10
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 3092 1870
Size: 73 331 818 4545
Fragments arranged by size:
4545 818 331 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 3088 4543 618
Size: 325 217 627 848 453 1455 1842
Fragments arranged by size:
1842 1455 848 627 453 325 217
BanII G_rGCy'C
Cuts at: 1391 2234 2281 2291 2455 2544 2778 3086 1391
Size: 843 47 10 164 89 234 308 4072
Fragments arranged by size:
4072 843 308 234 164 89 47 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2172 3050 928
Size: 971 273 878 3645
Fragments arranged by size:
3645 971 878 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2960 3659 4869 2960
Size: 699 1210 3858
Fragments arranged by size:
3858 1210 699
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3059 4378 136
Size: 107 122 71 631 605 1387 1319 1525
Fragments arranged by size:
1525 1387 1319 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2234 2281 2291 3828 4984 1790
Size: 424 20 47 10 1537 1156 2573
Fragments arranged by size:
2573 1537 1156 424 47 20 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2966 4427 1239
Size: 981 746 1461 2579
Fragments arranged by size:
2579 1461 981 746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2485 3892 4757 5055 2485
Size: 1407 865 298 3197
Fragments arranged by size:
3197 1407 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2589 3574 3956 4591
Size: 53 83 186 936 1056 985 382 635
Cuts at: 4591 275
Size: 1451
Fragments arranged by size:
1451 1056 985 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2568 4200 4598 4872 5019 5430 2568
Size: 1632 398 274 147 411 2905
Fragments arranged by size:
2905 1632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2482 1186
Size: 1296 4471
BseYI C'CCAG_C
Cuts at: 2284 2290 2454 4970 5466 2284
Size: 6 164 2516 496 2585
Fragments arranged by size:
2585 2516 496 164 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2580 1163
Size: 573 844 4350
Fragments arranged by size:
4350 844 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 2259 2824 3278 3371 2259
Size: 565 454 93 4655
Fragments arranged by size:
4655 565 454 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2234 2281 2291 2455 2544 2778
Size: 399 424 20 47 10 164 89 234
Cuts at: 2778 3086 3828 3913 4984 1391
Size: 308 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 308 234 164
89 85 47 20 10
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2718 3122 844
Size: 1874 404 3489
Fragments arranged by size:
3489 1874 404
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2223 4262 4436 65
Size: 1183 975 2039 174 1396
Fragments arranged by size:
2039 1396 1183 975 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 2243 4411 4598 1700
Size: 110 286 147 2168 187 2869
Fragments arranged by size:
2869 2168 286 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2265 794
Size: 1471 4296
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2595 3097 3464 3849 3866 5318 5404 5416
Size: 623 502 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 623 502 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2114 2475 513
Size: 552 939 110 361 3805
Fragments arranged by size:
3805 939 552 361 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2783 3283 4077 4097 2783
Size: 500 794 20 4453
Fragments arranged by size:
4453 794 500 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DraIII CAC_nnn'GTG
Cuts at: 2815 2815
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3060 3214
Size: 54 1425 50 275 18 268 962 154
Cuts at: 3214 4103 5732 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 962 889 275 268 154 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 3697 1353
Size: 1044 1300 3423
Fragments arranged by size:
3423 1300 1044
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2220 2966 3830 4427 5214 5482
Size: 393 427 161 746 864 597 787 268
Cuts at: 5482 1239
Size: 1524
Fragments arranged by size:
1524 864 787 746 597 427 393 268
161
EcoO109I rG'GnC_Cy
Cuts at: 2083 2231 2278 2083
Size: 148 47 5572
Fragments arranged by size:
5572 148 47
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
EcoRV GAT'ATC
Cuts at: 2887 2887
Size: 5767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2408 2408
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2097 2702 4914 1678
Size: 419 605 2212 2531
Fragments arranged by size:
2531 2212 605 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2836 2868 4408
Size: 32 135 32 825 32 407 32 1540
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1540 825 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 2639 3092 2639
Size: 453 5314
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 4881 5065 1305
Size: 987 2589 184 2007
Fragments arranged by size:
2589 2007 987 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3062 10
Size: 54 1425 343 1230 2715
Fragments arranged by size:
2715 1425 1230 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2975 3725 4084 4243 518
Size: 648 843 966 750 359 159 2042
Fragments arranged by size:
2042 966 843 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2556 3183 3860 5008 5253 1606
Size: 950 627 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 677 627 245
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 5767
NcoI C'CATG_G
Cuts at: 513 1065 2004 2475 513
Size: 552 939 471 3805
Fragments arranged by size:
3805 939 552 471
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3125 4670 1132
Size: 97 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2680 1348
Size: 1332 4435
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PfoI T'CCnGG_A
Cuts at: 2236 2236
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 3836 3868 5349 5381 2741
Size: 32 1063 32 1481 32 3127
Fragments arranged by size:
3127 1481 1063 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2230 2277 2287 2230
Size: 47 10 5710
Fragments arranged by size:
5710 47 10
PstI C_TGCA'G
Cuts at: 830 2715 3119 830
Size: 1885 404 3478
Fragments arranged by size:
3478 1885 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2711 3115 4250 4931 5122 826
Size: 219 1666 404 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 681 404 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3059 3059
Size: 5767
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2276 3094 1872
Size: 404 818 4545
Fragments arranged by size:
4545 818 404
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2464 3871 4772 5034 820
Size: 197 926 521 1407 901 262 1553
Fragments arranged by size:
1553 1407 926 901 521 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SrfI GCCC'GGGC
Cuts at: 2276 2276
Size: 5767
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 3021 1517
Size: 1504 4263
StyI C'CwwG_G
Cuts at: 513 1065 2004 2475 3206 513
Size: 552 939 471 731 3074
Fragments arranged by size:
3074 939 731 552 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 2600 2796 3794 3811 3964 4149
Size: 1255 879 141 196 998 17 153 185
Cuts at: 4149 4594 325
Size: 445 1498
Fragments arranged by size:
1498 1255 998 879 445 196 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2274 3092 1870
Size: 404 818 4545
Fragments arranged by size:
4545 818 404
XmnI GAAnn'nnTTC
Cuts at: 2756 3896 2756
Size: 1140 4627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfoI SwaI XcmI