Restriction map for
pFN21AB6469
(Circular) (Six-base) MAPSORT of: pFN21AB6469.seq Check: 3197 from: 1 to: 5677
pFN21AB6469 5677 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2703 3487 278
Size: 53 83 186 936 1167 784 2468
Fragments arranged by size:
2468 1167 936 784 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2286 2998 2286
Size: 712 4965
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2777 3740 5124 5392 1632
Size: 1145 963 1384 268 1917
Fragments arranged by size:
1917 1384 1145 963 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2235 2473 4576 5553 1203
Size: 1032 238 2103 977 1327
Fragments arranged by size:
2103 1327 1032 977 238
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2552 1166
Size: 1386 4291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
AvrII C'CTAG_G
Cuts at: 2395 2395
Size: 5677
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2286 2998 4453 618
Size: 325 217 627 499 712 1455 1842
Fragments arranged by size:
1842 1455 712 627 499 325 217
BanII G_rGCy'C
Cuts at: 1391 2996 1391
Size: 1605 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2695 2765 928
Size: 971 796 70 3840
Fragments arranged by size:
3840 971 796 70
BbvCI CC'TCA_GC
Cuts at: 2505 2505
Size: 5677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3868 3902 1903
Size: 34 50 34 1847 34 3678
Fragments arranged by size:
3678 1847 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 2561 3738 4894 1790
Size: 771 1177 1156 2573
Fragments arranged by size:
2573 1177 1156 771
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3408 4366 442
Size: 416 53 83 399 24 1991 958 1753
Fragments arranged by size:
1991 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4337 1239
Size: 3098 2579
Bpu10I CC'TnA_GC
Cuts at: 2505 2505
Size: 5677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2476 3468 493
Size: 711 1272 992 2702
Fragments arranged by size:
2702 1272 992 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2700 3484 3866 4501
Size: 53 83 186 936 1167 784 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1451 1167 936 784 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2972 1186
Size: 1786 3891
BseYI C'CCAG_C
Cuts at: 2568 4880 5376 2568
Size: 2312 496 2869
Fragments arranged by size:
2869 2312 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2804 1163
Size: 573 306 762 4036
Fragments arranged by size:
4036 762 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2561 2996 3738 3823 4894 1391
Size: 399 771 435 742 85 1071 2174
Fragments arranged by size:
2174 1071 771 742 435 399 85
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2337 3566 5296 2337
Size: 1229 1730 2718
Fragments arranged by size:
2718 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2718 3032 844
Size: 1874 314 3489
Fragments arranged by size:
3489 1874 314
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 4172 4346 65
Size: 1183 1071 1853 174 1396
Fragments arranged by size:
1853 1396 1183 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 2591 2620 2444
Size: 147 29 5501
Fragments arranged by size:
5501 147 29
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2590 2808 513
Size: 552 1525 218 3382
Fragments arranged by size:
3382 1525 552 218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 3193 3987 4007 2025
Size: 1168 794 20 3695
Fragments arranged by size:
3695 1168 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642
Size: 54 1425 50 275 18 1294 889 1629
Cuts at: 5642 8
Size: 43
Fragments arranged by size:
1629 1425 1294 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3607 1353
Size: 1527 727 3423
Fragments arranged by size:
3423 1527 727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2777 3740 4337 5124 5392 1239
Size: 393 1145 963 597 787 268 1524
Fragments arranged by size:
1524 1145 963 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2297 2392 2297
Size: 95 5582
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
EcoRV GAT'ATC
Cuts at: 2539 2626 2539
Size: 87 5590
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4318 4350 4392 4424 1373
Size: 32 135 32 2746 32 42 32 2626
Fragments arranged by size:
2746 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2615 748
Size: 1867 3810
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 2290 3002 2290
Size: 712 4965
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2071 2477 2494 4791 4975 1305
Size: 766 406 17 2297 184 2007
Fragments arranged by size:
2297 2007 766 406 184 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2378 2423 2442 2552 3635 3994
Size: 648 1147 65 45 19 110 1083 359
Cuts at: 3994 4153 518
Size: 159 2042
Fragments arranged by size:
2042 1147 1083 648 359 159 110 65
45 19
MspA1I CmG'CkG
Cuts at: 1606 2572 2692 3093 3770 4918 5163 1606
Size: 966 120 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 401 245 120
NcoI C'CATG_G
Cuts at: 513 1065 2590 513
Size: 552 1525 3600
Fragments arranged by size:
3600 1525 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2239 3035 4580 1132
Size: 97 1010 796 1545 2229
Fragments arranged by size:
2229 1545 1010 796 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 2235 4576 2235
Size: 2341 3336
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 2776 1108
Size: 936 732 4009
Fragments arranged by size:
4009 936 732
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PmlI CAC'GTG
Cuts at: 2476 2476
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2392 2392
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2514 2546 2514
Size: 32 5645
PstI C_TGCA'G
Cuts at: 830 2697 2733 3029 830
Size: 1867 36 296 3478
Fragments arranged by size:
3478 1867 296 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2692 2692
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 2733 3029 2733
Size: 296 5381
ScaI AGT'ACT
Cuts at: 2145 3925 5637 2145
Size: 1780 1712 2185
Fragments arranged by size:
2185 1780 1712
SexAI A'CCwGG_T
Cuts at: 2583 2583
Size: 5677
SfcI C'TryA_G
Cuts at: 826 1045 2406 2693 2729 3025 4160 4841 5032
Size: 219 1361 287 36 296 1135 681 191
Cuts at: 5032 826
Size: 1471
Fragments arranged by size:
1471 1361 1135 681 296 287 219 191
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2747 1517
Size: 1230 4447
StyI C'CwwG_G
Cuts at: 513 1065 2395 2590 3116 513
Size: 552 1330 195 526 3074
Fragments arranged by size:
3074 1330 552 526 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2143 2784 3923
Size: 274 80 33 51 1443 166 641 1139
Cuts at: 3923 5635 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1139 641 274 166 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2770 2011
Size: 759 4918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2701 3485 276
Size: 53 83 186 936 1167 784 2468
Fragments arranged by size:
2468 1167 936 784 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI