Restriction map for
pFN21AB6468
(Circular) (Six-base) MAPSORT of: pFN21AB6468.seq Check: 5287 from: 1 to: 5767
pFN21AB6468 5767 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2223 3110 1937
Size: 286 887 4594
Fragments arranged by size:
4594 887 286
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2407 3830 5214 5482 1632
Size: 735 40 1423 1384 268 1917
Fragments arranged by size:
1917 1423 1384 735 268 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2150 1166
Size: 984 4783
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 3510 5082 3510
Size: 1572 4195
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3086 1391
Size: 1695 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2549 928
Size: 971 135 515 4146
Fragments arranged by size:
4146 971 515 135
BbvCI CC'TCA_GC
Cuts at: 2709 2709
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2041 2075 2980 3014 3958 3992 1903
Size: 34 104 34 905 34 944 34 3678
Fragments arranged by size:
3678 944 905 104 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2734 2734
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2929 2958 5762 2929
Size: 29 2804 2934
Fragments arranged by size:
2934 2804 29
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2488 3498 4456
Size: 416 53 83 399 24 1071 1010 958
Cuts at: 4456 442
Size: 1753
Fragments arranged by size:
1753 1071 1010 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 2349 4427 1239
Size: 909 201 2078 2579
Fragments arranged by size:
2579 2078 909 201
Bpu10I CC'TnA_GC
Cuts at: 2709 2709
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 2165 5761 2165
Size: 3596 2171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 2857 1186
Size: 1546 125 4096
Fragments arranged by size:
4096 1546 125
BseYI C'CCAG_C
Cuts at: 4970 5466 4970
Size: 496 5271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1996 3020 1163
Size: 573 260 1024 3910
Fragments arranged by size:
3910 1024 573 260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 3217 3978 5006 1540
Size: 275 186 64 1152 761 1028 2301
Fragments arranged by size:
2301 1152 1028 761 275 186 64
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 2736 3278 3371 2736
Size: 542 93 5132
Fragments arranged by size:
5132 542 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3086 3828 3913 4984 1391
Size: 399 1296 742 85 1071 2174
Fragments arranged by size:
2174 1296 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 2173 2358 3656 5386 2173
Size: 185 1298 1730 2554
Fragments arranged by size:
2554 1730 1298 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 4262 4436 65
Size: 1183 999 2015 174 1396
Fragments arranged by size:
2015 1396 1183 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2312 794
Size: 1518 4249
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2429 2929 2958 3097 3464 3849 3866 5318
Size: 457 500 29 139 367 385 17 1452
Cuts at: 5318 5404 5416 5762 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 500 457 385 367 346 139
86 29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4015
Fragments arranged by size:
4015 939 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2046 2347 3283 4077 4097 2046
Size: 301 936 794 20 3716
Fragments arranged by size:
3716 936 794 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2536 2571 529
Size: 35 1972 35 3725
Fragments arranged by size:
3725 1972 35 35
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2611 3214
Size: 54 1425 50 275 18 264 517 603
Cuts at: 3214 4103 5732 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 603 517 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2807 2889 3697 1353
Size: 1454 82 808 3423
Fragments arranged by size:
3423 1454 808 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2349 2367 2407 3830 4427 5214
Size: 393 516 201 18 40 1423 597 787
Cuts at: 5214 5482 1239
Size: 268 1524
Fragments arranged by size:
1524 1423 787 597 516 393 268 201
40 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 2825
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2543 2302
Size: 241 5526
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2025 4914 1678
Size: 347 2889 2531
Fragments arranged by size:
2889 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2950 2982 4408
Size: 32 135 32 902 32 444 32 1426
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1426 902 444 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3111 3357 669
Size: 988 281 915 258 246 3079
Fragments arranged by size:
3079 988 915 281 258 246
HindIII A'AGCT_T
Cuts at: 748 2415 748
Size: 1667 4100
HpaI GTT'AAC
Cuts at: 2853 3357 2853
Size: 504 5263
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2534 4881 5065 1305
Size: 1229 2347 184 2007
Fragments arranged by size:
2347 2007 1229 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2613 10
Size: 54 1425 343 781 3164
Fragments arranged by size:
3164 1425 781 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2178 3725 4084 4243 518
Size: 648 984 28 1547 359 159 2042
Fragments arranged by size:
2042 1547 984 648 359 159 28
MspA1I CmG'CkG
Cuts at: 1606 2903 3183 3860 5008 5253 1606
Size: 1297 280 677 1148 245 2120
Fragments arranged by size:
2120 1297 1148 677 280 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4015
Fragments arranged by size:
4015 939 552 261
NdeI CA'TA_TG
Cuts at: 387 2890 387
Size: 2503 3264
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2736 2736
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3125 4670 1132
Size: 97 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2903 2903
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2224 3115 4250 4931 5122 826
Size: 219 1179 891 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 891 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2265 2952 3206 513
Size: 552 939 261 687 254 3074
Fragments arranged by size:
3074 939 687 552 261 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI