Restriction map for
pFN21AB6458
(Circular) (Six-base) MAPSORT of: pFN21AB6458.seq Check: 4439 from: 1 to: 5758
pFN21AB6458 5758 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 3101 1937
Size: 292 872 4594
Fragments arranged by size:
4594 872 292
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 2764 2997 3821 5205 5473 1632
Size: 632 500 233 824 1384 268 1917
Fragments arranged by size:
1917 1384 824 632 500 268 233
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 2197 4487 2197
Size: 2290 3468
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2780 2790 1184
Size: 1596 10 4152
Fragments arranged by size:
4152 1596 10
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaI G_GGCC'C
Cuts at: 2726 2726
Size: 5758
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2328 3067 3293 3445 5554 1493
Size: 835 739 226 152 2109 1697
Fragments arranged by size:
2109 1697 835 739 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 3083 1870
Size: 73 265 875 4545
Fragments arranged by size:
4545 875 265 73
BamHI G'GATC_C
Cuts at: 2650 3088 3455 2650
Size: 438 367 4953
Fragments arranged by size:
4953 438 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 4534 618
Size: 325 217 627 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2726 3077 1391
Size: 1335 351 4072
Fragments arranged by size:
4072 1335 351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BbvCI CC'TCA_GC
Cuts at: 2322 2322
Size: 5758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4369 136
Size: 107 122 71 631 605 337 2360 1525
Fragments arranged by size:
2360 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2263 5753 2263
Size: 3490 2268
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2726 3819 4975 1790
Size: 936 1093 1156 2573
Fragments arranged by size:
2573 1156 1093 936
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2474 2693 3489
Size: 416 53 83 399 24 1057 219 796
Cuts at: 3489 4447 442
Size: 958 1753
Fragments arranged by size:
1753 1057 958 796 416 399 219 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 4418 1239
Size: 1523 1656 2579
Fragments arranged by size:
2579 1656 1523
Bpu10I CC'TnA_GC
Cuts at: 2322 2322
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 3883 4748 5046 2438
Size: 1445 865 298 3150
Fragments arranged by size:
3150 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2353 4421 882
Size: 617 854 2068 2219
Fragments arranged by size:
2219 2068 854 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 2649 5752 2649
Size: 3103 2655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 2567 1186
Size: 1306 75 4377
Fragments arranged by size:
4377 1306 75
BseYI C'CCAG_C
Cuts at: 2832 4961 5457 2832
Size: 2129 496 3133
Fragments arranged by size:
3133 2129 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2205 2934 1163
Size: 573 469 729 3987
Fragments arranged by size:
3987 729 573 469
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2726 3077 3819 3904 4975 1391
Size: 399 936 351 742 85 1071 2174
Fragments arranged by size:
2174 1071 936 742 399 351 85
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 3113 844
Size: 1210 1059 3489
Fragments arranged by size:
3489 1210 1059
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2399 4253 4427 65
Size: 1183 1151 1854 174 1396
Fragments arranged by size:
1854 1396 1183 1151 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2840 2840
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2263 2650 3088 3455 3840 3857 5309 5395
Size: 291 387 438 367 385 17 1452 86
Cuts at: 5395 5407 5753 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 438 387 385 367 346 291
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2343 2848 513
Size: 552 939 339 505 3423
Fragments arranged by size:
3423 939 552 505 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2514 2697 2940 3274 4068 4088 2514
Size: 183 243 334 794 20 4184
Fragments arranged by size:
4184 794 334 243 183 20
ClaI AT'CG_AT
Cuts at: 1554 2337 1554
Size: 783 4975
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2183 2218 529
Size: 35 1619 35 4069
Fragments arranged by size:
4069 1619 35 35
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2819 2819
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3023 3205 4094
Size: 54 1425 50 275 18 1193 182 889
Cuts at: 4094 5723 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1193 889 275 182 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 2582 3688 1353
Size: 887 342 1106 3423
Fragments arranged by size:
3423 1106 887 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2762 2764 2997 3821 4418 5205
Size: 393 632 498 2 233 824 597 787
Cuts at: 5205 5473 1239
Size: 268 1524
Fragments arranged by size:
1524 824 787 632 597 498 393 268
233 2
EcoO109I rG'GnC_Cy
Cuts at: 2722 2723 2800 2722
Size: 1 77 5680
Fragments arranged by size:
5680 77 1
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 5758
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 4399 4431 4473
Size: 32 135 32 611 32 2184 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 611 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 4872 5056 1305
Size: 981 2586 184 2007
Fragments arranged by size:
2586 2007 981 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3025 10
Size: 54 1425 343 1193 2743
Fragments arranged by size:
2743 1425 1193 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2091 3716 4075 4234 518
Size: 648 925 1625 359 159 2042
Fragments arranged by size:
2042 1625 925 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2784 3174 3851 4999 5244 1606
Size: 435 743 390 677 1148 245 2120
Fragments arranged by size:
2120 1148 743 677 435 390 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2848 513
Size: 552 939 844 3423
Fragments arranged by size:
3423 939 844 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2767 3116 4661 1132
Size: 97 1538 349 1545 2229
Fragments arranged by size:
2229 1545 1538 349 97
PasI CC'CwG_GG
Cuts at: 1348 2734 1348
Size: 1386 4372
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2012 2044 3827 3859 5340 5372 2012
Size: 32 1783 32 1481 32 2398
Fragments arranged by size:
2398 1783 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2722 2722
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2692 2965 3110 830
Size: 1862 273 145 3478
Fragments arranged by size:
3478 1862 273 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2688 2961 3106 4241 4922 5113 826
Size: 219 1643 273 145 1135 681 191 1471
Fragments arranged by size:
1643 1471 1135 681 273 219 191 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 3862 4763 5025 820
Size: 197 926 510 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 510 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 2767 3116 1132
Size: 1635 349 3774
Fragments arranged by size:
3774 1635 349
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2848 3197 513
Size: 552 939 844 349 3074
Fragments arranged by size:
3074 939 844 552 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 3785 3802 3955 4140 4585 325
Size: 1255 843 1362 17 153 185 445 1498
Fragments arranged by size:
1498 1362 1255 843 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 4004 5716
Size: 274 80 33 51 1443 296 1731 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1731 1712 1443 296 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 5718 5750 2829
Size: 32 2857 32 2837
Fragments arranged by size:
2857 2837 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI