Restriction map for
pFN21AB6453
(Circular) (Six-base) MAPSORT of: pFN21AB6453.seq Check: 4791 from: 1 to: 5659
pFN21AB6453 5659 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 2612 3722 5106 5374 1632
Size: 680 300 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 680 300 268
AfeI AGC'GCT
Cuts at: 2564 2564
Size: 5659
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2335 3402 4974 2335
Size: 1067 1572 3020
Fragments arranged by size:
3020 1572 1067
ApaI G_GGCC'C
Cuts at: 2129 2129
Size: 5659
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2486 2968 3194 3346 5455 1493
Size: 993 482 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 482 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2537 2570 2537
Size: 33 5626
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2766 2844 2980 4435 618
Size: 325 217 627 979 78 136 1455 1842
Fragments arranged by size:
1842 1455 979 627 325 217 136 78
BanII G_rGCy'C
Cuts at: 1391 2055 2129 2978 1391
Size: 664 74 849 4072
Fragments arranged by size:
4072 849 664 74
BbeI G_GCGC'C
Cuts at: 2848 2848
Size: 5659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 928
Size: 971 147 4541
Fragments arranged by size:
4541 971 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3850 3884 1903
Size: 34 50 34 1829 34 3678
Fragments arranged by size:
3678 1829 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2458 2926 1548
Size: 910 468 4281
Fragments arranged by size:
4281 910 468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2193 5654 2193
Size: 3461 2198
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2420 3720 4876 1790
Size: 339 291 1300 1156 2573
Fragments arranged by size:
2573 1300 1156 339 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2163 3390 4348
Size: 416 53 83 399 24 746 1227 958
Cuts at: 4348 442
Size: 1753
Fragments arranged by size:
1753 1227 958 746 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 4319 1239
Size: 1331 1749 2579
Fragments arranged by size:
2579 1749 1331
Bpu10I CC'TnA_GC
Cuts at: 2054 2054
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3784 4649 4947 2174
Size: 1610 865 298 2886
Fragments arranged by size:
2886 1610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2285 2625 4322 882
Size: 617 786 340 1697 2219
Fragments arranged by size:
2219 1697 786 617 340
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2845 3466 3848 4483
Size: 53 83 186 936 1312 621 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1312 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2462 2465 1186
Size: 1276 3 4380
Fragments arranged by size:
4380 1276 3
BseYI C'CCAG_C
Cuts at: 2128 2565 4862 5358 2128
Size: 437 2297 496 2429
Fragments arranged by size:
2429 2297 496 437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 3109 3870 4898 1540
Size: 275 186 90 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186 90
BsiHKAI G_wGCw'C
Cuts at: 2055 3720 3805 4876 2055
Size: 1665 85 1071 2838
Fragments arranged by size:
2838 1665 1071 85
BsmI GAATG_Cn'
Cuts at: 2695 2824 3170 3263 2695
Size: 129 346 93 5091
Fragments arranged by size:
5091 346 129 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2129 2420 2978 3720 3805 4876
Size: 399 265 74 291 558 742 85 1071
Cuts at: 4876 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 558 399 291 265 85
74
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2188 2890 3014 844
Size: 1344 702 124 3489
Fragments arranged by size:
3489 1344 702 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2030 3546 1394
Size: 429 207 1516 3507
Fragments arranged by size:
3507 1516 429 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 4303 4490 1700
Size: 110 746 1747 187 2869
Fragments arranged by size:
2869 1747 746 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2743 794
Size: 1949 3710
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2193 2901 2989 3356 3741 3758 5210 5296
Size: 221 708 88 367 385 17 1452 86
Cuts at: 5296 5308 5654 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 708 385 367 346 221 88
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3175 3969 3989 2929
Size: 246 794 20 4599
Fragments arranged by size:
4599 794 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DraIII CAC_nnn'GTG
Cuts at: 2171 2171
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 3106 3995
Size: 54 1425 50 275 18 258 1018 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1018 889 275 258 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2088 3106 1537
Size: 275 276 1018 4090
Fragments arranged by size:
4090 1018 276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3589 1353
Size: 719 1517 3423
Fragments arranged by size:
3423 1517 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 4246 4620 4766 387
Size: 1064 138 462 2195 374 146 1280
Fragments arranged by size:
2195 1280 1064 462 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2053
Size: 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2570 2612 3722 4319 5106 5374
Size: 393 680 258 42 1110 597 787 268
Cuts at: 5374 1239
Size: 1524
Fragments arranged by size:
1524 1110 787 680 597 393 268 258
42
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2526 2138
Size: 388 5271
EcoO109I rG'GnC_Cy
Cuts at: 2125 2143 2596 2914 2125
Size: 18 453 318 4870
Fragments arranged by size:
4870 453 318 18
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2566 2716 2848 4806 1678
Size: 888 150 132 1958 2531
Fragments arranged by size:
2531 1958 888 150 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2806 2838 4300 4332 4374
Size: 32 135 32 1234 32 1462 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 1462 1234 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KasI G'GCGC_C
Cuts at: 2844 2844
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3617 3976 4135 518
Size: 648 2451 359 159 2042
Fragments arranged by size:
2451 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2321 3075 3752 4900 5145 1606
Size: 715 754 677 1148 245 2120
Fragments arranged by size:
2120 1148 754 715 677 245
NarI GG'CG_CC
Cuts at: 2845 2845
Size: 5659
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5107
NdeI CA'TA_TG
Cuts at: 387 2515 387
Size: 2128 3531
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2143
Size: 5659
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2125 2125
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2739 3011 830
Size: 1909 272 3478
Fragments arranged by size:
3478 1909 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2321 2321
Size: 5659
SacI G_AGCT'C
Cuts at: 2055 2055
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2735 3007 4142 4823 5014 826
Size: 219 1690 272 1135 681 191 1471
Fragments arranged by size:
1690 1471 1135 681 272 219 191
SfoI GGC'GCC
Cuts at: 2846 2846
Size: 5659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 3763 4664 4926 820
Size: 197 926 246 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2673 2716 2910 3098 513
Size: 552 1608 43 194 188 3074
Fragments arranged by size:
3074 1608 552 194 188 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2182 3686 3703 3856 4041 4486 325
Size: 1255 602 1504 17 153 185 445 1498
Fragments arranged by size:
1504 1498 1255 602 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 2577 3788 2577
Size: 1211 4448
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI