Restriction map for
pFN21AB6448
(Circular) (Six-base) MAPSORT of: pFN21AB6448.seq Check: 9340 from: 1 to: 5752
pFN21AB6448 5752 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2602
Size: 5752
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3051 3879 4252 1563
Size: 1488 828 373 3063
Fragments arranged by size:
3063 1488 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3815 5199 5467 1632
Size: 2183 1384 268 1917
Fragments arranged by size:
2183 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2709 1166
Size: 1543 4209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaI G_GGCC'C
Cuts at: 3018 3018
Size: 5752
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2375 3061 3287 3439 5548 1493
Size: 882 686 226 152 2109 1697
Fragments arranged by size:
2109 1697 882 686 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2514 4306 160
Size: 872 1482 1792 1606
Fragments arranged by size:
1792 1606 1482 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2154 3077 1870
Size: 73 109 102 923 4545
Fragments arranged by size:
4545 923 109 102 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2071 2905 3018 3071 1391
Size: 661 19 834 113 53 4072
Fragments arranged by size:
4072 834 661 113 53 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2226 3644 4854 2226
Size: 1418 1210 3124
Fragments arranged by size:
3124 1418 1210
BclI T'GATC_A
Cuts at: 1548 3033 1548
Size: 1485 4267
BfrBI ATG'CAT
Cuts at: 2652 2652
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4363 136
Size: 107 122 71 631 605 337 2354 1525
Fragments arranged by size:
2354 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 3018 3813 4969 1790
Size: 1228 795 1156 2573
Fragments arranged by size:
2573 1228 1156 795
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2089 3483 4441
Size: 416 53 83 399 24 672 1394 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1394 958 672 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2085 4412 1239
Size: 825 21 2327 2579
Fragments arranged by size:
2579 2327 825 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 2873 5746 2873
Size: 2873 2879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2665 4185 4583 4857 5004 5415 2665
Size: 1520 398 274 147 411 3002
Fragments arranged by size:
3002 1520 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2177 2195 2433 1186
Size: 886 105 18 238 4505
Fragments arranged by size:
4505 886 238 105 18
BseYI C'CCAG_C
Cuts at: 2538 4955 5451 2538
Size: 2417 496 2839
Fragments arranged by size:
2839 2417 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2071 2905 3018 3071 3813 3898
Size: 399 262 19 834 113 53 742 85
Cuts at: 3898 4969 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 834 742 399 262 113 85
53 19
BspEI T'CCGG_A
Cuts at: 2665 5415 2665
Size: 2750 3002
BspHI T'CATG_A
Cuts at: 2380 3641 5371 2380
Size: 1261 1730 2761
Fragments arranged by size:
2761 1730 1261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2568 2602 3107 844
Size: 1409 315 34 505 3489
Fragments arranged by size:
3489 1409 505 315 34
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2269 4247 4421 65
Size: 1183 1021 1978 174 1396
Fragments arranged by size:
1978 1396 1183 1021 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 2280 96
Size: 2184 3568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2832 2832
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747
Size: 1110 367 385 17 1452 86 12 346
Cuts at: 5747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2952 3199
Size: 54 1425 50 275 18 498 624 247
Cuts at: 3199 4088 5717 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 624 498 275 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 3682 1353
Size: 1068 1261 3423
Fragments arranged by size:
3423 1261 1068
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 4339 4713 4859 387
Size: 1064 138 447 2303 374 146 1280
Fragments arranged by size:
2303 1280 1064 447 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2085 3815 4412 5199 5467 1239
Size: 393 432 21 1730 597 787 268 1524
Fragments arranged by size:
1730 1524 787 597 432 393 268 21
EcoO109I rG'GnC_Cy
Cuts at: 2233 3014 3015 2233
Size: 781 1 4970
Fragments arranged by size:
4970 781 1
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 2102 4258 2102
Size: 2156 3596
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 2581 2613 4393
Size: 32 135 32 850 32 127 32 1780
Cuts at: 4393 4425 4467 4499 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1780 850 135 127 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 4866 5050 1305
Size: 1077 2484 184 2007
Fragments arranged by size:
2484 2007 1077 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 2954 10
Size: 54 1425 343 498 624 2808
Fragments arranged by size:
2808 1425 624 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2573 2604 2709 2825 2879 3710 4069
Size: 648 1407 31 105 116 54 831 359
Cuts at: 4069 4228 518
Size: 159 2042
Fragments arranged by size:
2042 1407 831 648 359 159 116 105
54 31
MspA1I CmG'CkG
Cuts at: 1606 2018 3168 3845 4993 5238 1606
Size: 412 1150 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 677 412 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NsiI A_TGCA'T
Cuts at: 2654 2654
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4084
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2233
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 3014 3014
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2403 2445 3104 830
Size: 1573 42 659 3478
Fragments arranged by size:
3478 1573 659 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2399 2441 3100 4235 4916 5107 826
Size: 219 1354 42 659 1135 681 191 1471
Fragments arranged by size:
1471 1354 1135 681 659 219 191 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2054 2156 3079 1872
Size: 182 102 923 4545
Fragments arranged by size:
4545 923 182 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 2257 3676 5
Size: 47 2205 1419 2081
Fragments arranged by size:
2205 2081 1419 47
StuI AGG'CCT
Cuts at: 1517 2990 1517
Size: 1473 4279
StyI C'CwwG_G
Cuts at: 513 1065 2004 2991 3191 513
Size: 552 939 987 200 3074
Fragments arranged by size:
3074 987 939 552 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2309 2721 3779 3796 3949 4134
Size: 1255 633 96 412 1058 17 153 185
Cuts at: 4134 4579 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1058 633 445 412 185 153
96 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 2307 2829
Size: 274 80 33 51 1443 303 27 522
Cuts at: 2829 3998 5710 96
Size: 1169 1712 138
Fragments arranged by size:
1712 1443 1169 522 303 274 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2777 2832 2777
Size: 55 5697
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2052 2154 3077 1870
Size: 182 102 923 4545
Fragments arranged by size:
4545 923 182 102
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI