Restriction map for
pFN21AB6446
(Circular) (Six-base) MAPSORT of: pFN21AB6446.seq Check: 7879 from: 1 to: 5746
pFN21AB6446 5746 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2054 2501 2575 2054
Size: 447 74 5225
Fragments arranged by size:
5225 447 74
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 2979 3067 2979
Size: 88 5658
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 3809 5193 5461 1632
Size: 671 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 671 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 2915 4475 2915
Size: 1560 4186
AleI CACnn'nnGTG
Cuts at: 1166 2798 1166
Size: 1632 4114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2852 1184
Size: 1668 4078
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2409 2574 3003 3071 1870
Size: 73 466 165 429 68 4545
Fragments arranged by size:
4545 466 429 165 73 68
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2711 2789 2979 3067
Size: 325 217 627 255 669 78 190 88
Cuts at: 3067 4522 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 669 627 325 255 217 190
88 78
BanII G_rGCy'C
Cuts at: 1391 2229 2410 2418 2581 3065 1391
Size: 838 181 8 163 484 4072
Fragments arranged by size:
4072 838 484 181 163 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2725 928
Size: 971 826 3949
Fragments arranged by size:
3949 971 826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2793 3638 4848 2793
Size: 845 1210 3691
Fragments arranged by size:
3691 1210 845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 2243 2826
Size: 107 122 71 631 605 442 129 583
Cuts at: 2826 4357 136
Size: 1531 1525
Fragments arranged by size:
1531 1525 631 605 583 442 129 122
107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 3807 4963 1790
Size: 2017 1156 2573
Fragments arranged by size:
2573 2017 1156
BmgBI CAC'GTC
Cuts at: 1318 2892 1318
Size: 1574 4172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 4406 1239
Size: 929 2238 2579
Fragments arranged by size:
2579 2238 929
Bpu10I CC'TnA_GC
Cuts at: 2090 2090
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 3871 4736 5034 2234
Size: 1637 865 298 2946
Fragments arranged by size:
2946 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2093 2671 2902 4409 882
Size: 617 594 578 231 1507 2219
Fragments arranged by size:
2219 1507 617 594 578 231
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2676 3553 3935 4570
Size: 53 83 186 936 1143 877 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1143 936 877 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2361 2391 1178
Size: 30 165 30 958 30 4533
Fragments arranged by size:
4533 958 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2415 2418 2445 2483 3029 1186
Size: 958 271 3 27 38 546 3903
Fragments arranged by size:
3903 958 546 271 38 27 3
BseYI C'CCAG_C
Cuts at: 2104 2693 2967 4949 5445 2104
Size: 589 274 1982 496 2405
Fragments arranged by size:
2405 1982 589 496 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2938 1163
Size: 573 1202 3971
Fragments arranged by size:
3971 1202 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2614 3196 3957 4985 1540
Size: 275 186 613 582 761 1028 2301
Fragments arranged by size:
2301 1028 761 613 582 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2346 2410 2492 3807 3892 4963 2136
Size: 210 64 82 1315 85 1071 2919
Fragments arranged by size:
2919 1315 1071 210 85 82 64
BsmI GAATG_Cn'
Cuts at: 2527 2548 3257 3350 2527
Size: 21 709 93 4923
Fragments arranged by size:
4923 709 93 21
BsmBI CGTCTCn'nnnn_
Cuts at: 2876 2876
Size: 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2229 2346 2410 2418 2492 2581
Size: 399 346 93 117 64 8 74 89
Cuts at: 2581 3065 3807 3892 4963 1391
Size: 484 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 346 117 93
89 85 74 64 8
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 2501 2575 3101 844
Size: 1210 447 74 526 3489
Fragments arranged by size:
3489 1210 526 447 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2544 4390 4577 1700
Size: 110 734 1846 187 2869
Fragments arranged by size:
2869 1846 734 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2533 2800 2975 2506
Size: 27 267 175 5277
Fragments arranged by size:
5277 267 175 27
BstYI r'GATC_y
Cuts at: 1972 2514 3076 3443 3828 3845 5297 5383 5395
Size: 542 562 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 562 542 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2823 2823
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DraIII CAC_nnn'GTG
Cuts at: 2140 2140
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2611 3193 4082
Size: 54 1425 50 275 18 781 582 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 781 582 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2611 3193 1537
Size: 275 799 582 4090
Fragments arranged by size:
4090 799 582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 2472 3676 1353
Size: 1101 18 1204 3423
Fragments arranged by size:
3423 1204 1101 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2671 4333 4707 4853 387
Size: 1064 138 1082 1662 374 146 1280
Fragments arranged by size:
1662 1280 1082 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2408 2408
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2303 3809 4406 5193 5461 1239
Size: 393 536 135 1506 597 787 268 1524
Fragments arranged by size:
1524 1506 787 597 536 393 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2465
Size: 5746
EcoO109I rG'GnC_Cy
Cuts at: 2201 2963 2201
Size: 762 4984
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 4387 4419 4461
Size: 32 135 32 1329 32 1454 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 1454 1329 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 2983 3071 2983
Size: 88 5658
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 2894 3704 4063 4222 518
Size: 648 1632 96 810 359 159 2042
Fragments arranged by size:
2042 1632 810 648 359 159 96
MspA1I CmG'CkG
Cuts at: 1606 2629 2952 2971 3162 3839 4987 5232 1606
Size: 1023 323 19 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 1023 677 323 245 191 19
NaeI GCC'GGC
Cuts at: 2546 2546
Size: 5746
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NgoMIV G'CCGG_C
Cuts at: 2544 2544
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 2611 3193 2611
Size: 582 5164
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2811 2811
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2201 2963 2201
Size: 762 4984
PshAI GACnn'nnGTC
Cuts at: 1613 2868 1613
Size: 1255 4491
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 2586 3098 830
Size: 1756 512 3478
Fragments arranged by size:
3478 1756 512
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2629 2952 2971 2629
Size: 323 19 5404
Fragments arranged by size:
5404 323 19
SacI G_AGCT'C
Cuts at: 2410 2410
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SanDI GG'GwC_CC
Cuts at: 2963 2963
Size: 5746
SapI GCTCTTCn'nnn_
Cuts at: 2472 2472
Size: 5746
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2582 3094 4229 4910 5101 826
Size: 219 1537 512 1135 681 191 1471
Fragments arranged by size:
1537 1471 1135 681 512 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2576 3005 3073 1872
Size: 704 429 68 4545
Fragments arranged by size:
4545 704 429 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 3850 4751 5013 820
Size: 197 926 306 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 4666
StyI C'CwwG_G
Cuts at: 513 1065 3185 513
Size: 552 2120 3074
Fragments arranged by size:
3074 2120 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2778 2926 3773 3790 3943 4128 4573
Size: 1255 1198 148 847 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 1198 847 445 185 153 148
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 2361 2393 5706 5738 2126
Size: 32 203 32 3313 32 2134
Fragments arranged by size:
3313 2134 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2065 1316
Size: 749 4997
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2648 2648
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 2574 3003 3071 1870
Size: 704 429 68 4545
Fragments arranged by size:
4545 704 429 68
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI