Restriction map for
pFN21AB6445
(Circular) (Six-base) MAPSORT of: pFN21AB6445.seq Check: 1724 from: 1 to: 5650
pFN21AB6445 5650 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2569 2287
Size: 282 5368
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 2655 2907 2993 1937
Size: 370 348 252 86 4594
Fragments arranged by size:
4594 370 348 252 86
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 3713 5097 5365 1632
Size: 503 1578 1384 268 1917
Fragments arranged by size:
1917 1578 1384 503 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2556 4549 5526 1203
Size: 1353 1993 977 1327
Fragments arranged by size:
1993 1353 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 2512 2732 4379 2512
Size: 220 1647 3783
Fragments arranged by size:
3783 1647 220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 2275 1184
Size: 882 209 4559
Fragments arranged by size:
4559 882 209
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaI G_GGCC'C
Cuts at: 2453 2453
Size: 5650
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2036 2531 2959 3185 3337 5446 1493
Size: 543 495 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 543 495 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2338 2362 2674 2975 1870
Size: 73 375 20 24 312 301 4545
Fragments arranged by size:
4545 375 312 301 73 24 20
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2861 2971 4426 618
Size: 325 217 627 500 574 110 1455 1842
Fragments arranged by size:
1842 1455 627 574 500 325 217 110
BanII G_rGCy'C
Cuts at: 1391 2090 2442 2453 2766 2894 2901 2969 1391
Size: 699 352 11 313 128 7 68 4072
Fragments arranged by size:
4072 699 352 313 128 68 11 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 4067
Fragments arranged by size:
4067 971 612
BbvCI CC'TCA_GC
Cuts at: 2816 2816
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2686 2720 3841 3875 1903
Size: 34 749 34 1121 34 3678
Fragments arranged by size:
3678 1121 749 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 4261 136
Size: 107 122 71 631 605 348 2241 1525
Fragments arranged by size:
2241 1525 631 605 348 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 2024 3067 2024
Size: 1043 4607
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2290 2453 3711 4867 1790
Size: 493 7 163 1258 1156 2573
Fragments arranged by size:
2573 1258 1156 493 163 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2293 3381 4339
Size: 416 53 83 399 24 876 1088 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 1088 958 876 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2104 4310 1239
Size: 865 2206 2579
Fragments arranged by size:
2579 2206 865
Bpu10I CC'TnA_GC
Cuts at: 2816 2816
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2420 3457 3839 4474
Size: 53 83 186 936 887 1037 382 635
Cuts at: 4474 275
Size: 1451
Fragments arranged by size:
1451 1037 936 887 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 1178
Size: 30 165 30 1350 30 4045
Fragments arranged by size:
4045 1350 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2450 1186
Size: 1117 147 4386
Fragments arranged by size:
4386 1117 147
BseYI C'CCAG_C
Cuts at: 2446 4853 5349 2446
Size: 2407 496 2747
Fragments arranged by size:
2747 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 1163
Size: 573 341 4736
Fragments arranged by size:
4736 573 341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 2636 2712 3711 3796 4867 2090
Size: 546 76 999 85 1071 2873
Fragments arranged by size:
2873 1071 999 546 85 76
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2283 2290 2442 2453 2636 2712
Size: 399 300 193 7 152 11 183 76
Cuts at: 2712 2766 2894 2901 2969 3711 3796 4867 1391
Size: 54 128 7 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 300 193 183 152
128 85 76 68 54 11 7 7
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 2569 2616 2667 2691 3005 844
Size: 1443 282 47 51 24 314 3489
Fragments arranged by size:
3489 1443 314 282 51 47 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2079 3537 1394
Size: 429 256 1458 3507
Fragments arranged by size:
3507 1458 429 256
BsrDI GCAATG_nn'
Cuts at: 65 1248 2796 4145 4319 65
Size: 1183 1548 1349 174 1396
Fragments arranged by size:
1548 1396 1349 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2919 4294 4481 1700
Size: 110 1109 1375 187 2869
Fragments arranged by size:
2869 1375 1109 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2833
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645
Size: 1008 367 385 17 1452 86 12 346
Cuts at: 5645 1972
Size: 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2886 2886
Size: 5650
BtgI C'CryG_G
Cuts at: 513 1065 2848 513
Size: 552 1783 3315
Fragments arranged by size:
3315 1783 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2382 487
Size: 683 56 1156 3755
Fragments arranged by size:
3755 1156 683 56
BtsI GCAGTG_nn'
Cuts at: 2236 2407 3166 3960 3980 2236
Size: 171 759 794 20 3906
Fragments arranged by size:
3906 794 759 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615
Size: 54 1425 50 275 18 1267 889 1629
Cuts at: 5615 8
Size: 43
Fragments arranged by size:
1629 1425 1267 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2088 2088
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2135 3713 4310 5097 5365 1239
Size: 393 472 31 1578 597 787 268 1524
Fragments arranged by size:
1578 1524 787 597 472 393 268 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2101 2101
Size: 5650
EcoO109I rG'GnC_Cy
Cuts at: 2148 2812 2148
Size: 664 4986
EcoRI G'AATT_C
Cuts at: 2036 2531 2959 3337 2036
Size: 495 428 378 4349
Fragments arranged by size:
4349 495 428 378
EcoRV GAT'ATC
Cuts at: 2938 2938
Size: 5650
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2718 2750 4291
Size: 32 135 32 926 32 188 32 1541
Cuts at: 4291 4323 4365 4397 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1541 926 188 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 2538 3249 2538
Size: 711 4939
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 4764 4948 1305
Size: 1359 2100 184 2007
Fragments arranged by size:
2100 2007 1359 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2561 2831 3608 3967 4126 518
Size: 648 1395 270 777 359 159 2042
Fragments arranged by size:
2042 1395 777 648 359 270 159
MspA1I CmG'CkG
Cuts at: 1606 2265 2850 3066 3743 4891 5136 1606
Size: 659 585 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 659 585 245 216
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2518 1348
Size: 1170 4480
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2684 2716 3719 3751 5232 5264 2684
Size: 32 1003 32 1481 32 3070
Fragments arranged by size:
3070 1481 1003 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2148
Size: 5650
PshAI GACnn'nnGTC
Cuts at: 1613 2597 1613
Size: 984 4666
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PspOMI G'GGCC_C
Cuts at: 2449 2449
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2688 3002 830
Size: 1858 314 3478
Fragments arranged by size:
3478 1858 314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SacI G_AGCT'C
Cuts at: 2090 2090
Size: 5650
SacII CC_GC'GG
Cuts at: 2851 2851
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 2164 3898 5610 2164
Size: 1734 1712 2204
Fragments arranged by size:
2204 1734 1712
SexAI A'CCwGG_T
Cuts at: 2508 2508
Size: 5650
SfcI C'TryA_G
Cuts at: 826 1045 2684 2998 4133 4814 5005 826
Size: 219 1639 314 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 681 314 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2320 2364 2977 1872
Size: 448 44 613 4545
Fragments arranged by size:
4545 613 448 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 3754 4655 4917 820
Size: 197 926 395 1416 901 262 1553
Fragments arranged by size:
1553 1416 926 901 395 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2187 1517
Size: 670 4980
StyI C'CwwG_G
Cuts at: 513 1065 2901 3089 513
Size: 552 1836 188 3074
Fragments arranged by size:
3074 1836 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 2082 2291 3677 3694 3847 4032
Size: 1255 468 34 209 1386 17 153 185
Cuts at: 4032 4477 325
Size: 445 1498
Fragments arranged by size:
1498 1386 1255 468 445 209 185 153
34 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2162 2838 3896
Size: 274 80 33 51 1443 185 676 1058
Cuts at: 3896 5608 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1058 676 274 185 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 2854 2886 5610 5642 2753
Size: 32 69 32 2724 32 2761
Fragments arranged by size:
2761 2724 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 2338 1943
Size: 395 5255
XmaI C'CCGG_G
Cuts at: 1870 2318 2362 2975 1870
Size: 448 44 613 4545
Fragments arranged by size:
4545 613 448 44
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SanDI SapI SfiI SfoI
SrfI SwaI XcmI