Restriction map for
pFN21AB6433
(Circular) (Six-base) MAPSORT of: pFN21AB6433.seq Check: 6955 from: 1 to: 5644
pFN21AB6433 5644 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2270 2270
Size: 5644
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 3707 5091 5359 1632
Size: 580 1495 1384 268 1917
Fragments arranged by size:
1917 1495 1384 580 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2444 2615 1166
Size: 1278 171 4195
Fragments arranged by size:
4195 1278 171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2278 1184
Size: 1094 4550
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaI G_GGCC'C
Cuts at: 2421 2780 2796 2421
Size: 359 16 5269
Fragments arranged by size:
5269 359 16
ApaLI G'TGCA_C
Cuts at: 2916 3701 4857 2916
Size: 785 1156 3703
Fragments arranged by size:
3703 1156 785
ApoI r'AATT_y
Cuts at: 1493 2318 2953 3179 3331 5440 1493
Size: 825 635 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2599 2723 2741 2969 1870
Size: 73 656 124 18 228 4545
Fragments arranged by size:
4545 656 228 124 73 18
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 2717 2737 2965 4420
Size: 325 217 627 486 444 20 228 1455
Cuts at: 4420 618
Size: 1842
Fragments arranged by size:
1842 1455 627 486 444 325 228 217
20
BanII G_rGCy'C
Cuts at: 1391 2079 2157 2368 2421 2780 2796 2963 1391
Size: 688 78 211 53 359 16 167 4072
Fragments arranged by size:
4072 688 359 211 167 78 53 16
BbeI G_GCGC'C
Cuts at: 2721 2721
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2101 2278 928
Size: 971 202 177 4294
Fragments arranged by size:
4294 971 202 177
BbvCI CC'TCA_GC
Cuts at: 2198 2198
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2191 2225 3835 3869 1903
Size: 34 50 34 170 34 1610 34 3678
Fragments arranged by size:
3678 1610 170 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 4255 136
Size: 107 122 71 631 605 599 1984 1525
Fragments arranged by size:
1984 1525 631 605 599 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 2162 3061 2162
Size: 899 4745
Bme1580I G_kGCm'C
Cuts at: 1790 2276 2421 2780 2796 2920 3705 4861 1790
Size: 486 145 359 16 124 785 1156 2573
Fragments arranged by size:
2573 1156 785 486 359 145 124 16
BmgBI CAC'GTC
Cuts at: 1318 2029 1318
Size: 711 4933
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 4304 1239
Size: 902 2163 2579
Fragments arranged by size:
2579 2163 902
Bpu10I CC'TnA_GC
Cuts at: 2198 2198
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2580 4307 882
Size: 617 1081 1727 2219
Fragments arranged by size:
2219 1727 1081 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2718 2748 3451 3833
Size: 53 83 186 936 1185 30 703 382
Cuts at: 3833 4468 275
Size: 635 1451
Fragments arranged by size:
1451 1185 936 703 635 382 186 83
53 30
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 1186
Size: 1110 4534
BseYI C'CCAG_C
Cuts at: 2048 2685 4847 5343 2048
Size: 637 2162 496 2349
Fragments arranged by size:
2349 2162 637 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 1163
Size: 573 483 4588
Fragments arranged by size:
4588 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2646 2920 3705 3790 4861 2157
Size: 489 274 785 85 1071 2940
Fragments arranged by size:
2940 1071 785 489 274 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2157 2276 2368 2421 2646 2780
Size: 399 289 78 119 92 53 225 134
Cuts at: 2780 2796 2920 2963 3705 3790 4861 1391
Size: 16 124 43 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 225 134 124
119 92 85 78 53 43 16
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2270 2999 844
Size: 1426 729 3489
Fragments arranged by size:
3489 1426 729
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2108 4139 4313 65
Size: 1183 860 2031 174 1396
Fragments arranged by size:
2031 1396 1183 860 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2734 2849 4288 4475 1700
Size: 110 924 115 1439 187 2869
Fragments arranged by size:
2869 1439 924 187 115 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 2704 2706 1925
Size: 779 2 4863
Fragments arranged by size:
4863 779 2
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2941 2941
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639
Size: 1002 367 385 17 1452 86 12 346
Cuts at: 5639 1972
Size: 1977
Fragments arranged by size:
1977 1452 1002 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2814 2877 2814
Size: 63 5581
BtgI C'CryG_G
Cuts at: 513 1065 2331 2358 2439 513
Size: 552 1266 27 81 3718
Fragments arranged by size:
3718 1266 552 81 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2772 487
Size: 683 56 1546 3359
Fragments arranged by size:
3359 1546 683 56
BtsI GCAGTG_nn'
Cuts at: 2869 3160 3954 3974 2869
Size: 291 794 20 4539
Fragments arranged by size:
4539 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DraIII CAC_nnn'GTG
Cuts at: 2242 2242
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2370 2436
Size: 54 1425 50 275 18 504 36 66
Cuts at: 2436 2511 3091 3980 5609 8
Size: 75 580 889 1629 43
Fragments arranged by size:
1629 1425 889 580 504 275 75 66
54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 4231 4605 4751 387
Size: 1064 138 444 2198 374 146 1280
Fragments arranged by size:
2198 1280 1064 444 374 146 138
EcoICRI GAG'CTC
Cuts at: 2155 2155
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2212 3707 4304 5091 5359 1239
Size: 393 509 71 1495 597 787 268 1524
Fragments arranged by size:
1524 1495 787 597 509 393 268 71
EcoO109I rG'GnC_Cy
Cuts at: 2417 2776 2777 2793 2417
Size: 359 1 16 5268
Fragments arranged by size:
5268 359 16 1
EcoRI G'AATT_C
Cuts at: 2318 2953 3331 2318
Size: 635 378 4631
Fragments arranged by size:
4631 635 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2721 4791 1678
Size: 1043 2070 2531
Fragments arranged by size:
2531 2070 1043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 4285 4317 4359
Size: 32 135 32 719 32 1962 32 42
Cuts at: 4359 4391 1373
Size: 32 2626
Fragments arranged by size:
2626 1962 719 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2843 2988 3234 669
Size: 988 281 905 145 246 3079
Fragments arranged by size:
3079 988 905 281 246 145
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2717 2717
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 2438 2513 10
Size: 54 1425 343 540 66 75 3141
Fragments arranged by size:
3141 1425 540 343 75 66 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2444 2479 2615 3602 3961 4120
Size: 648 861 417 35 136 987 359 159
Cuts at: 4120 518
Size: 2042
Fragments arranged by size:
2042 987 861 648 417 359 159 136
35
MspA1I CmG'CkG
Cuts at: 1606 2041 2161 2856 3060 3737 4885 5130 1606
Size: 435 120 695 204 677 1148 245 2120
Fragments arranged by size:
2120 1148 695 677 435 245 204 120
NaeI GCC'GGC
Cuts at: 2851 2851
Size: 5644
NarI GG'CG_CC
Cuts at: 2718 2718
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2358 513
Size: 552 1293 3799
Fragments arranged by size:
3799 1293 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2849 2849
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2136 2574 3002 4547 1132
Size: 97 907 438 428 1545 2229
Fragments arranged by size:
2229 1545 907 438 428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2198 2230 3713 3745 5226 5258 2198
Size: 32 1483 32 1481 32 2584
Fragments arranged by size:
2584 1483 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2838 1613
Size: 1225 4419
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2417 2776 2792 2417
Size: 359 16 5269
Fragments arranged by size:
5269 359 16
PstI C_TGCA'G
Cuts at: 830 2391 2996 830
Size: 1561 605 3478
Fragments arranged by size:
3478 1561 605
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2041 2161 2041
Size: 120 5524
SacI G_AGCT'C
Cuts at: 2157 2157
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SexAI A'CCwGG_T
Cuts at: 2637 2637
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2143 2387 2992 4127 4808 4999 826
Size: 219 1098 244 605 1135 681 191 1471
Fragments arranged by size:
1471 1135 1098 681 605 244 219 191
SfoI GGC'GCC
Cuts at: 2719 2719
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2743 2971 1872
Size: 871 228 4545
Fragments arranged by size:
4545 871 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2280 2358 2487 3083 513
Size: 552 1215 78 129 596 3074
Fragments arranged by size:
3074 1215 596 552 129 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 2272 2935 3671 3688 3841 4026
Size: 1255 621 71 663 736 17 153 185
Cuts at: 4026 4471 325
Size: 445 1498
Fragments arranged by size:
1498 1255 736 663 621 445 185 153
71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 3890 5602
Size: 274 80 33 51 1443 267 1646 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1646 1443 274 267 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2365 2834 2011
Size: 354 469 4821
Fragments arranged by size:
4821 469 354
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2741 2969 1870
Size: 871 228 4545
Fragments arranged by size:
4545 871 228
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SrfI SwaI