Restriction map for
pFN21AB6430
(Circular) (Six-base) MAPSORT of: pFN21AB6430.seq Check: 3193 from: 1 to: 5734
pFN21AB6430 5734 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3797 5181 5449 1632
Size: 2165 1384 268 1917
Fragments arranged by size:
2165 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 2653 2862 4633 5610 1203
Size: 1450 209 1771 977 1327
Fragments arranged by size:
1771 1450 1327 977 209
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 2401 4463 2401
Size: 2062 3672
AleI CACnn'nnGTG
Cuts at: 1166 2582 1166
Size: 1416 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AlwNI CAG_nnn'CTG
Cuts at: 2332 3477 5049 2332
Size: 1145 1572 3017
Fragments arranged by size:
3017 1572 1145
ApaI G_GGCC'C
Cuts at: 2068 2707 2068
Size: 639 5095
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 3059 1870
Size: 73 116 1000 4545
Fragments arranged by size:
4545 1000 116 73
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2236 2836 3055 4510
Size: 325 217 627 245 204 600 219 1455
Cuts at: 4510 618
Size: 1842
Fragments arranged by size:
1842 1455 627 600 325 245 219 217
204
BanII G_rGCy'C
Cuts at: 1391 2068 2096 2151 2707 2781 2898 3029 3053
Size: 677 28 55 556 74 117 131 24
Cuts at: 3053 1391
Size: 4072
Fragments arranged by size:
4072 677 556 131 117 74 55 28
24
BbeI G_GCGC'C
Cuts at: 2036 2240 2036
Size: 204 5530
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2913 928
Size: 971 116 898 3749
Fragments arranged by size:
3749 971 898 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 4345 136
Size: 107 122 71 631 605 874 1799 1525
Fragments arranged by size:
1799 1525 874 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2514 5729 2514
Size: 3215 2519
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2579 2707 3795 4951 1790
Size: 278 511 128 1088 1156 2573
Fragments arranged by size:
2573 1156 1088 511 278 128
BmgBI CAC'GTC
Cuts at: 1318 2773 1318
Size: 1455 4279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2591 2627 2865 3009 4394 1239
Size: 1121 231 36 238 144 1385 2579
Fragments arranged by size:
2579 1385 1121 238 231 144 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 2780 4397 882
Size: 617 576 705 1617 2219
Fragments arranged by size:
2219 1617 705 617 576
BsaAI yAC'GTr
Cuts at: 493 1204 2173 2580 2656 2865 3525 493
Size: 711 969 407 76 209 660 2702
Fragments arranged by size:
2702 969 711 660 407 209 76
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2033 2237 2413
Size: 53 83 186 936 479 21 204 176
Cuts at: 2413 3541 3923 4558 275
Size: 1128 382 635 1451
Fragments arranged by size:
1451 1128 936 635 479 382 204 186
176 83 53 21
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2403 2433 2468 2498 2564
Size: 30 165 30 1000 30 35 30 66
Cuts at: 2564 2594 2982 3012 1178
Size: 30 388 30 3900
Fragments arranged by size:
3900 1000 388 165 66 35 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2408 2864 1186
Size: 1105 117 456 4056
Fragments arranged by size:
4056 1105 456 117
BseYI C'CCAG_C
Cuts at: 2700 4937 5433 2700
Size: 2237 496 3001
Fragments arranged by size:
3001 2237 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2894 1163
Size: 573 1158 4003
Fragments arranged by size:
4003 1158 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 3000 3184 3945 4973 1540
Size: 275 186 139 860 184 761 1028 2301
Fragments arranged by size:
2301 1028 860 761 275 186 184 139
BsiHKAI G_wGCw'C
Cuts at: 2781 3029 3795 3880 4951 2781
Size: 248 766 85 1071 3564
Fragments arranged by size:
3564 1071 766 248 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2096 2151 2579 2707 2781 2898
Size: 399 278 28 55 428 128 74 117
Cuts at: 2898 3029 3053 3795 3880 4951 1391
Size: 131 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 278 131 128
117 85 74 55 28 24
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 2487 3089 844
Size: 1487 156 602 3489
Fragments arranged by size:
3489 1487 602 156
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 2632 1925
Size: 707 5027
BssSI C'ACGA_G
Cuts at: 2780 3794 4806 2780
Size: 1014 1012 3708
Fragments arranged by size:
3708 1014 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2191 2332 794
Size: 1397 141 4196
Fragments arranged by size:
4196 1397 141
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2514 3064 3431 3816 3833 5285 5371 5383
Size: 542 550 367 385 17 1452 86 12
Cuts at: 5383 5729 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 550 542 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2693 2693
Size: 5734
BtgI C'CryG_G
Cuts at: 513 1065 2140 2163 2231 2240 2422 513
Size: 552 1075 23 68 9 182 3825
Fragments arranged by size:
3825 1075 552 182 68 23 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 487
Size: 683 56 917 4078
Fragments arranged by size:
4078 917 683 56
BtsI GCAGTG_nn'
Cuts at: 2502 3250 4044 4064 2502
Size: 748 794 20 4172
Fragments arranged by size:
4172 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2004 2039 529
Size: 35 1440 35 4224
Fragments arranged by size:
4224 1440 35 35
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2538 3181
Size: 54 1425 50 275 18 307 401 643
Cuts at: 3181 4070 5699 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 643 401 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2137 3181 1537
Size: 275 325 1044 4090
Fragments arranged by size:
4090 1044 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2268 3017 3664 1353
Size: 698 217 749 647 3423
Fragments arranged by size:
3423 749 698 647 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2779 3027 2779
Size: 248 5486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2591 2627 2865 3009 3797 4394
Size: 393 728 231 36 238 144 788 597
Cuts at: 4394 5181 5449 1239
Size: 787 268 1524
Fragments arranged by size:
1524 788 787 728 597 393 268 238
231 144 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2471
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2064 2065 2476 2704 2064
Size: 1 411 228 5094
Fragments arranged by size:
5094 411 228 1
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2036 2121 2240 4881 1678
Size: 358 85 119 2641 2531
Fragments arranged by size:
2641 2531 358 119 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2387 2403 2419
Size: 32 135 32 620 32 163 16 16
Cuts at: 2419 2435 2468 2500 2982 3014 4375 4407 4449
Size: 16 33 32 482 32 1361 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 1361 620 482 163 135 42 33
32 32 32 32 32 32 32 16
16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KasI G'GCGC_C
Cuts at: 2032 2236 2032
Size: 204 5530
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 4848 5032 1305
Size: 1125 2418 184 2007
Fragments arranged by size:
2418 2007 1125 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3912
Fragments arranged by size:
3912 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2582 3692 4051 4210 518
Size: 648 996 420 1110 359 159 2042
Fragments arranged by size:
2042 1110 996 648 420 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2233 2424 2498 2746 2828 2978 3150
Size: 536 91 191 74 248 82 150 172
Cuts at: 3150 3827 4975 5220 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 248 245 191 172
150 91 82 74
NarI GG'CG_CC
Cuts at: 2033 2237 2033
Size: 204 5530
NcoI C'CATG_G
Cuts at: 513 1065 2163 513
Size: 552 1098 4084
Fragments arranged by size:
4084 1098 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2707 1348
Size: 1359 4375
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2773 1108
Size: 1665 4069
PfoI T'CCnGG_A
Cuts at: 2486 2486
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PmlI CAC'GTG
Cuts at: 2580 2656 2865 2580
Size: 76 209 5449
Fragments arranged by size:
5449 209 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2476
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2064 2703 2064
Size: 639 5095
PstI C_TGCA'G
Cuts at: 830 2328 2631 2800 3086 830
Size: 1498 303 169 286 3478
Fragments arranged by size:
3478 1498 303 286 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2828 2978 2828
Size: 150 5584
SacI G_AGCT'C
Cuts at: 2781 3029 2781
Size: 248 5486
SacII CC_GC'GG
Cuts at: 2143 2234 2425 2143
Size: 91 191 5452
Fragments arranged by size:
5452 191 91
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SapI GCTCTTCn'nnn_
Cuts at: 3017 3017
Size: 5734
SbfI CC_TGCA'GG
Cuts at: 2800 3086 2800
Size: 286 5448
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2324 2627 2796 2979 3082 4217 4898
Size: 219 1279 303 169 183 103 1135 681
Cuts at: 4898 5089 826
Size: 191 1471
Fragments arranged by size:
1471 1279 1135 681 303 219 191 183
169 103
SfoI GGC'GCC
Cuts at: 2034 2238 2034
Size: 204 5530
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3838 4739 5001 820
Size: 197 926 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 2794 1517
Size: 1277 4457
StyI C'CwwG_G
Cuts at: 513 1065 2015 2163 2292 3173 513
Size: 552 950 148 129 881 3074
Fragments arranged by size:
3074 950 881 552 148 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 2233 3761 3778 3931 4116 4561
Size: 1255 513 140 1528 17 153 185 445
Cuts at: 4561 325
Size: 1498
Fragments arranged by size:
1528 1498 1255 513 445 185 153 140
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 5694 5726 2564
Size: 32 3098 32 2572
Fragments arranged by size:
3098 2572 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2170 2384 2941 2170
Size: 214 557 4963
Fragments arranged by size:
4963 557 214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI