Restriction map for
pFN21AB6427
(Circular) (Six-base) MAPSORT of: pFN21AB6427.seq Check: 6907 from: 1 to: 5623
pFN21AB6427 5623 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2860 2860
Size: 5623
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3433 278
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2966 1937
Size: 1029 4594
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 3750 4123 1563
Size: 2187 373 3063
Fragments arranged by size:
3063 2187 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 2297 2459 3686 5070 5338 1632
Size: 532 133 162 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 532 268 162 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 4352 4352
Size: 5623
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5623
AlwNI CAG_nnn'CTG
Cuts at: 2035 2149 2404 3366 4938 2035
Size: 114 255 962 1572 2720
Fragments arranged by size:
2720 1572 962 255 114
ApaLI G'TGCA_C
Cuts at: 2247 3680 4836 2247
Size: 1433 1156 3034
Fragments arranged by size:
3034 1433 1156
ApoI r'AATT_y
Cuts at: 1493 2654 2932 3158 3310 5419 1493
Size: 1161 278 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 278 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2302 2948 1870
Size: 73 359 646 4545
Fragments arranged by size:
4545 646 359 73
AvrII C'CTAG_G
Cuts at: 2371 2371
Size: 5623
BamHI G'GATC_C
Cuts at: 2953 3320 2953
Size: 367 5256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2944 4399 618
Size: 325 217 627 1157 1455 1842
Fragments arranged by size:
1842 1455 1157 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2487 2942 1391
Size: 651 445 455 4072
Fragments arranged by size:
4072 651 455 445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2823 928
Size: 971 924 3728
Fragments arranged by size:
3728 971 924
BbvCI CC'TCA_GC
Cuts at: 2586 2586
Size: 5623
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4725 3515
Size: 1210 4413
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4234 136
Size: 107 122 71 631 605 2562 1525
Fragments arranged by size:
2562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5618 5618
Size: 5623
BlpI GC'TnA_GC
Cuts at: 2036 3040 2036
Size: 1004 4619
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2799 3684 4840 1790
Size: 461 548 885 1156 2573
Fragments arranged by size:
2573 1156 885 548 461
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 2539 2773
Size: 416 53 83 399 24 827 295 234
Cuts at: 2773 3354 4312 442
Size: 581 958 1753
Fragments arranged by size:
1753 958 827 581 416 399 295 234
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2630 4283 1239
Size: 1166 225 1653 2579
Fragments arranged by size:
2579 1653 1166 225
Bpu10I CC'TnA_GC
Cuts at: 2195 2586 2195
Size: 391 5232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 4286 882
Size: 617 1203 1584 2219
Fragments arranged by size:
2219 1584 1203 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3430 3812 4447 275
Size: 53 83 186 936 1897 382 635 1451
Fragments arranged by size:
1897 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4056 4454 4728 4875 5286 4056
Size: 398 274 147 411 4393
Fragments arranged by size:
4393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 1178
Size: 30 165 30 931 30 4437
Fragments arranged by size:
4437 931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2290 2477 2660 1186
Size: 1104 187 183 4149
Fragments arranged by size:
4149 1104 187 183
BseYI C'CCAG_C
Cuts at: 2142 2803 2880 4826 5322 2142
Size: 661 77 1946 496 2443
Fragments arranged by size:
2443 1946 661 496 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5050
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2251 2309 2487 2541 3684 3769 4840 2042
Size: 209 58 178 54 1143 85 1071 2825
Fragments arranged by size:
2825 1143 1071 209 178 85 58 54
BsmI GAATG_Cn'
Cuts at: 2908 3134 3227 2908
Size: 226 93 5304
Fragments arranged by size:
5304 226 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2165 2165
Size: 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2251 2309 2487 2541 2799 2942
Size: 399 252 209 58 178 54 258 143
Cuts at: 2942 3684 3769 4840 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 258 252 209 178
143 85 58 54
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 2522 2860 2978 844
Size: 1622 56 338 118 3489
Fragments arranged by size:
3489 1622 338 118 56
BsrBI CCG'CTC
Cuts at: 1394 1823 3510 1394
Size: 429 1687 3507
Fragments arranged by size:
3507 1687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2909 4267 4454 1700
Size: 110 1099 1358 187 2869
Fragments arranged by size:
2869 1358 1099 187 110
BsrGI T'GTAC_A
Cuts at: 96 2662 96
Size: 2566 3057
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 2798 3683 4695 2798
Size: 885 1012 3726
Fragments arranged by size:
3726 1012 885
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5623
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5623
BstXI CCAn_nnnn'nTGG
Cuts at: 2787 2840 2787
Size: 53 5570
BstYI r'GATC_y
Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618
Size: 981 367 385 17 1452 86 12 346
Cuts at: 5618 1972
Size: 1977
Fragments arranged by size:
1977 1452 981 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2338 2451 513
Size: 552 939 165 169 113 3685
Fragments arranged by size:
3685 939 552 169 165 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4884
Fragments arranged by size:
4884 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3933 3953 3139
Size: 794 20 4809
Fragments arranged by size:
4809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DraIII CAC_nnn'GTG
Cuts at: 2626 2626
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2842 2919
Size: 54 1425 50 275 18 177 835 77
Cuts at: 2919 3070 3959 5588 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 889 835 275 177 151 77
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 3553 1353
Size: 1082 1118 3423
Fragments arranged by size:
3423 1118 1082
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2606 2914 4210 4584 4730 387
Size: 1064 138 1017 308 1296 374 146 1280
Fragments arranged by size:
1296 1280 1064 1017 374 308 146 138
EcoICRI GAG'CTC
Cuts at: 2040 2485 2040
Size: 445 5178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2297 2405 2459 2630 3686 4283
Size: 393 532 133 108 54 171 1056 597
Cuts at: 4283 5070 5338 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1056 787 597 532 393 268 171
133 108 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 2315
Size: 5623
EcoO109I rG'GnC_Cy
Cuts at: 2238 2401 2511 2238
Size: 163 110 5350
Fragments arranged by size:
5350 163 110
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
EcoRV GAT'ATC
Cuts at: 2026 2026
Size: 5623
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 4770 1678
Size: 3092 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 4264 4296 4338
Size: 32 135 32 526 32 2134 32 42
Cuts at: 4338 4370 1373
Size: 32 2626
Fragments arranged by size:
2626 2134 526 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 2967 3213 669
Size: 988 281 589 440 246 3079
Fragments arranged by size:
3079 988 589 440 281 246
HindIII A'AGCT_T
Cuts at: 748 2283 748
Size: 1535 4088
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 3222 3222
Size: 5623
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4737 4921 1305
Size: 3432 184 2007
Fragments arranged by size:
3432 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3624
Fragments arranged by size:
3624 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2070 2228 3581 3940 4099 518
Size: 648 904 158 1353 359 159 2042
Fragments arranged by size:
2042 1353 904 648 359 159 158
MspA1I CmG'CkG
Cuts at: 1606 2035 2254 2550 2608 2847 2880 3039 3716
Size: 429 219 296 58 239 33 159 677
Cuts at: 3716 4864 5109 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 429 296 245 239 219
159 58 33
NcoI C'CATG_G
Cuts at: 513 1065 2004 2169 513
Size: 552 939 165 3967
Fragments arranged by size:
3967 939 552 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5623
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2981 4526 1132
Size: 97 1752 1545 2229
Fragments arranged by size:
2229 1752 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5623
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 1108
Size: 1041 4582
PfoI T'CCnGG_A
Cuts at: 2570 2570
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 5205 5237 3692
Size: 32 1481 32 4078
Fragments arranged by size:
4078 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2238 2401 2511 2238
Size: 163 110 5350
Fragments arranged by size:
5350 163 110
PshAI GACnn'nnGTC
Cuts at: 1613 2318 1613
Size: 705 4918
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PspXI vC'TCGA_Gb
Cuts at: 2302 2302
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2871 2975 830
Size: 2041 104 3478
Fragments arranged by size:
3478 2041 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
PvuII CAG'CTG
Cuts at: 2035 2254 2550 2608 2035
Size: 219 296 58 5050
Fragments arranged by size:
5050 296 219 58
SacI G_AGCT'C
Cuts at: 2042 2487 2042
Size: 445 5178
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SanDI GG'GwC_CC
Cuts at: 2238 2238
Size: 5623
SapI GCTCTTCn'nnn_
Cuts at: 2435 2435
Size: 5623
SbfI CC_TGCA'GG
Cuts at: 2871 2975 2871
Size: 104 5519
ScaI AGT'ACT
Cuts at: 2600 3871 5583 2600
Size: 1271 1712 2640
Fragments arranged by size:
2640 1712 1271
SexAI A'CCwGG_T
Cuts at: 2622 2622
Size: 5623
SfcI C'TryA_G
Cuts at: 826 1045 2743 2867 2971 4106 4787 4978 826
Size: 219 1698 124 104 1135 681 191 1471
Fragments arranged by size:
1698 1471 1135 681 219 191 124 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 2909 1700
Size: 1209 4414
SmaI CCC'GGG
Cuts at: 1872 2950 1872
Size: 1078 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 3727 4628 4890 820
Size: 197 926 359 1425 901 262 1553
Fragments arranged by size:
1553 1425 926 901 359 262 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2981 1132
Size: 1849 3774
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 1517
Size: 1062 4561
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2169 2371 2542 3062 513
Size: 552 939 6 159 202 171 520 3074
Fragments arranged by size:
3074 939 552 520 202 171 159 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 2351 2521 2716 2795 2872 3650
Size: 1255 762 9 170 195 79 77 778
Cuts at: 3650 3667 3820 4005 4450 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 778 762 445 195 185 170
153 79 77 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 2662 3869
Size: 274 80 33 51 1443 621 64 1207
Cuts at: 3869 5581 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1207 621 274 138 80 64
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2334 2366 5583 5615 2334
Size: 32 3217 32 2342
Fragments arranged by size:
3217 2342 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2236 1316
Size: 920 4703
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2914 2914
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 2302 1943
Size: 359 5264
XmaI C'CCGG_G
Cuts at: 1870 2948 1870
Size: 1078 4545
XmnI GAAnn'nnTTC
Cuts at: 2444 2678 3752 2444
Size: 234 1074 4315
Fragments arranged by size:
4315 1074 234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3431 276
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
RsrII SacII SfiI SfoI SrfI SwaI