Restriction map for
pFN21AB6426
(Circular) (Six-base) MAPSORT of: pFN21AB6426.seq Check: 6096 from: 1 to: 5563
pFN21AB6426 5563 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 1937
Size: 969 4594
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 5010 5278 1632
Size: 1994 1384 268 1917
Fragments arranged by size:
1994 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 4462 5439 1203
Size: 3259 977 1327
Fragments arranged by size:
3259 1327 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 2413 4292 2413
Size: 1879 3684
AleI CACnn'nnGTG
Cuts at: 1166 2250 2453 1166
Size: 1084 203 4276
Fragments arranged by size:
4276 1084 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 2470 3306 4878 2470
Size: 836 1572 3155
Fragments arranged by size:
3155 1572 836
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2872 3098 3250 5359 1493
Size: 1379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2216 2888 1870
Size: 73 177 96 672 4545
Fragments arranged by size:
4545 672 177 96 73
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2711 2884 4339 618
Size: 325 217 627 259 665 173 1455 1842
Fragments arranged by size:
1842 1455 665 627 325 259 217 173
BanII G_rGCy'C
Cuts at: 1391 2882 1391
Size: 1491 4072
BbeI G_GCGC'C
Cuts at: 2050 2715 2050
Size: 665 4898
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2634 928
Size: 971 735 3857
Fragments arranged by size:
3857 971 735
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2627 2661 3754 3788 1903
Size: 34 690 34 1093 34 3678
Fragments arranged by size:
3678 1093 690 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2394 4174 136
Size: 107 122 71 631 605 722 1780 1525
Fragments arranged by size:
1780 1525 722 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2376 5558 2376
Size: 3182 2381
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 3624 4780 1790
Size: 1834 1156 2573
Fragments arranged by size:
2573 1834 1156
BmgBI CAC'GTC
Cuts at: 1318 2509 1318
Size: 1191 4372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3294 4252 442
Size: 416 53 83 399 24 1877 958 1753
Fragments arranged by size:
1877 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2835 4223 1239
Size: 1596 1388 2579
Fragments arranged by size:
2579 1596 1388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 3688 4553 4851 2744
Size: 944 865 298 3456
Fragments arranged by size:
3456 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 2726 4226 882
Size: 617 672 555 1500 2219
Fragments arranged by size:
2219 1500 672 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3354 493
Size: 711 2150 2702
Fragments arranged by size:
2702 2150 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2047 2538 2712 3370
Size: 53 83 186 936 514 491 174 658
Cuts at: 3370 3752 4387 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 658 635 514 491 382 186
174 83 53
BsaWI w'CCGG_w
Cuts at: 3996 4394 4668 4815 5226 3996
Size: 398 274 147 411 4333
Fragments arranged by size:
4333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2547 2577 1178
Size: 30 165 30 1144 30 4164
Fragments arranged by size:
4164 1144 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5563
BseYI C'CCAG_C
Cuts at: 2517 4766 5262 2517
Size: 2249 496 2818
Fragments arranged by size:
2818 2249 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2531 1163
Size: 573 795 4195
Fragments arranged by size:
4195 795 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2533 3013 3774 4802 1540
Size: 275 186 532 480 761 1028 2301
Fragments arranged by size:
2301 1028 761 532 480 275 186
BsiHKAI G_wGCw'C
Cuts at: 2614 2747 3624 3709 4780 2614
Size: 133 877 85 1071 3397
Fragments arranged by size:
3397 1071 877 133 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
BsmBI CGTCTCn'nnnn_
Cuts at: 2290 2545 2290
Size: 255 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2614 2747 2882 3624 3709 4780 1391
Size: 399 824 133 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 824 742 399 135 133 85
BspEI T'CCGG_A
Cuts at: 5226 5226
Size: 5563
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 2490 2918 844
Size: 1574 72 428 3489
Fragments arranged by size:
3489 1574 428 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 1394
Size: 429 1627 3507
Fragments arranged by size:
3507 1627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4058 4232 65
Size: 1183 2810 174 1396
Fragments arranged by size:
2810 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4207 4394 1700
Size: 110 2397 187 2869
Fragments arranged by size:
2869 2397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 2246 3623 4635 2246
Size: 1377 1012 3174
Fragments arranged by size:
3174 1377 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5563
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2376 2754 2893 3260 3645 3662 5114 5200
Size: 404 378 139 367 385 17 1452 86
Cuts at: 5200 5212 5558 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 404 385 378 367 346 139
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2236 2389 2533 513
Size: 552 1171 153 144 3543
Fragments arranged by size:
3543 1171 552 153 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2338 487
Size: 683 56 1112 3712
Fragments arranged by size:
3712 1112 683 56
BtsI GCAGTG_nn'
Cuts at: 3079 3873 3893 3079
Size: 794 20 4749
Fragments arranged by size:
4749 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2671 2706 529
Size: 35 2107 35 3386
Fragments arranged by size:
3386 2107 35 35
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DraIII CAC_nnn'GTG
Cuts at: 2620 2620
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2537 4570 809
Size: 507 1221 2033 1802
Fragments arranged by size:
2033 1802 1221 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2358 2568
Size: 54 1425 50 275 18 241 287 210
Cuts at: 2568 3010 3899 5528 8
Size: 442 889 1629 43
Fragments arranged by size:
1629 1425 889 442 287 275 241 210
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3493 1353
Size: 2140 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2449 2836 4150 4524 4670 387
Size: 1064 138 860 387 1314 374 146 1280
Fragments arranged by size:
1314 1280 1064 860 387 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2835 3626 4223 5010 5278 1239
Size: 393 1203 791 597 787 268 1524
Fragments arranged by size:
1524 1203 791 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2467 2467
Size: 5563
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2608 2188
Size: 420 5143
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 2050 2715 4710 1678
Size: 372 665 1995 2531
Fragments arranged by size:
2531 1995 665 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 4204 4236 4278
Size: 32 135 32 827 32 1773 32 42
Cuts at: 4278 4310 1373
Size: 32 2626
Fragments arranged by size:
2626 1773 827 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3153 669
Size: 988 281 969 246 3079
Fragments arranged by size:
3079 988 969 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KasI G'GCGC_C
Cuts at: 2046 2711 2046
Size: 665 4898
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4677 4861 1305
Size: 729 2643 184 2007
Fragments arranged by size:
2643 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 3003
Fragments arranged by size:
3003 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2250 2453 3521 3880 4039 518
Size: 648 1084 203 1068 359 159 2042
Fragments arranged by size:
2042 1084 1068 648 359 203 159
MspA1I CmG'CkG
Cuts at: 1606 2659 2979 3656 4804 5049 1606
Size: 1053 320 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 320 245
NarI GG'CG_CC
Cuts at: 2047 2712 2047
Size: 665 4898
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5011
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2921 4466 1132
Size: 97 1692 1545 2229
Fragments arranged by size:
2229 1692 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5563
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2780 2812 2780
Size: 32 5531
PstI C_TGCA'G
Cuts at: 830 2415 2915 830
Size: 1585 500 3478
Fragments arranged by size:
3478 1585 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
PvuII CAG'CTG
Cuts at: 2659 2659
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 2078 3811 5523 2078
Size: 1733 1712 2118
Fragments arranged by size:
2118 1733 1712
SexAI A'CCwGG_T
Cuts at: 2616 2616
Size: 5563
SfcI C'TryA_G
Cuts at: 826 1045 2411 2911 4046 4727 4918 826
Size: 219 1366 500 1135 681 191 1471
Fragments arranged by size:
1471 1366 1135 681 500 219 191
SfoI GGC'GCC
Cuts at: 2048 2713 2048
Size: 665 4898
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 3667 4568 4830 820
Size: 197 926 816 908 901 262 1553
Fragments arranged by size:
1553 926 908 901 816 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 2397 1517
Size: 880 4683
StyI C'CwwG_G
Cuts at: 513 1065 2172 3002 513
Size: 552 1107 830 3074
Fragments arranged by size:
3074 1107 830 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3590 3607 3760 3945 4390 325
Size: 1255 2010 17 153 185 445 1498
Fragments arranged by size:
2010 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 3809 5521
Size: 274 80 33 51 1443 99 1733 1712
Cuts at: 5521 96
Size: 138
Fragments arranged by size:
1733 1712 1443 274 138 99 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2547 2579 5523 5555 2547
Size: 32 2944 32 2555
Fragments arranged by size:
2944 2555 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2656 2722 2656
Size: 66 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 2225 2324 3692 2225
Size: 99 1368 4096
Fragments arranged by size:
4096 1368 99
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI