Restriction map for
pFN21AB6424
(Circular) (Six-base) MAPSORT of: pFN21AB6424.seq Check: 5895 from: 1 to: 5725
pFN21AB6424 5725 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2909 2909
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2659 3068 1937
Size: 722 409 4594
Fragments arranged by size:
4594 722 409
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 2677 4624 5601 1203
Size: 1474 1947 977 1327
Fragments arranged by size:
1947 1474 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2240 4279 160
Size: 872 1208 2039 1606
Fragments arranged by size:
2039 1606 1208 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 3050 1870
Size: 73 1107 4545
Fragments arranged by size:
4545 1107 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3044 1391
Size: 1653 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2189 3617 4827 2189
Size: 1428 1210 3087
Fragments arranged by size:
3087 1428 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BfrBI ATG'CAT
Cuts at: 2188 2275 2188
Size: 87 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 3786 4942 1790
Size: 1996 1156 2573
Fragments arranged by size:
2573 1996 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2556 3456 4414
Size: 416 53 83 399 24 1139 900 958
Cuts at: 4414 442
Size: 1753
Fragments arranged by size:
1753 1139 958 900 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 4385 1239
Size: 1338 1808 2579
Fragments arranged by size:
2579 1808 1338
Bpu10I CC'TnA_GC
Cuts at: 2956 2956
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 3850 4715 5013 2211
Size: 1639 865 298 2923
Fragments arranged by size:
2923 1639 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2851 3532 3914 4549
Size: 53 83 186 936 1318 681 382 635
Cuts at: 4549 275
Size: 1451
Fragments arranged by size:
1451 1318 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5725
BseYI C'CCAG_C
Cuts at: 2526 4928 5424 2526
Size: 2402 496 2827
Fragments arranged by size:
2827 2402 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2793 2937 1163
Size: 573 1057 144 3951
Fragments arranged by size:
3951 1057 573 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3786 3871 4942 3786
Size: 85 1071 4569
Fragments arranged by size:
4569 1071 85
BsmI GAATG_Cn'
Cuts at: 2551 3236 3329 2551
Size: 685 93 4947
Fragments arranged by size:
4947 685 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2858 2858
Size: 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3044 3786 3871 4942 1391
Size: 399 1254 742 85 1071 2174
Fragments arranged by size:
2174 1254 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 2800 2935 3614 5344 2800
Size: 135 679 1730 3181
Fragments arranged by size:
3181 1730 679 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 3080 844
Size: 2065 171 3489
Fragments arranged by size:
3489 2065 171
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2754 4369 4556 1700
Size: 110 944 1615 187 2869
Fragments arranged by size:
2869 1615 944 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720
Size: 1083 367 385 17 1452 86 12 346
Cuts at: 5720 1972
Size: 1977
Fragments arranged by size:
1977 1452 1083 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 3241 4035 4055 3241
Size: 794 20 4911
Fragments arranged by size:
4911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 4061 5690
Size: 54 1425 50 275 18 1342 889 1629
Cuts at: 5690 8
Size: 43
Fragments arranged by size:
1629 1425 1342 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2629 3655 1353
Size: 1276 1026 3423
Fragments arranged by size:
3423 1276 1026
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2577 3788 4385 5172 5440 1239
Size: 393 945 1211 597 787 268 1524
Fragments arranged by size:
1524 1211 945 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2292 2292
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
EcoRV GAT'ATC
Cuts at: 2689 2689
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 4872 1678
Size: 3194 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4839 5023 1305
Size: 3534 184 2007
Fragments arranged by size:
3534 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3903
Fragments arranged by size:
3903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3683 4042 4201 518
Size: 648 1815 702 359 159 2042
Fragments arranged by size:
2042 1815 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3141 3818 4966 5211 1606
Size: 1535 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 677 245
NaeI GCC'GGC
Cuts at: 2756 2756
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NgoMIV G'CCGG_C
Cuts at: 2754 2754
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NsiI A_TGCA'T
Cuts at: 2190 2277 2190
Size: 87 5638
NspI r_CATG'y
Cuts at: 1132 1229 2079 2279 2681 3083 4628 1132
Size: 97 850 200 402 402 1545 2229
Fragments arranged by size:
2229 1545 850 402 402 200 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 2677 4624 2677
Size: 1947 3778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2634 1108
Size: 1526 4199
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2292 2292
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2134 2166 2134
Size: 32 5693
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2829 3073 4208 4889 5080 826
Size: 219 1784 244 1135 681 191 1471
Fragments arranged by size:
1784 1471 1135 681 244 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 3829 4730 4992 820
Size: 197 926 283 1603 901 262 1553
Fragments arranged by size:
1603 1553 926 901 283 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 2279 3083 1132
Size: 1147 804 3774
Fragments arranged by size:
3774 1147 804
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 3164 513
Size: 552 2099 3074
Fragments arranged by size:
3074 2099 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2290 3752 3769 3922 4107 4552 325
Size: 1255 710 1462 17 153 185 445 1498
Fragments arranged by size:
1498 1462 1255 710 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 2824 3854 2824
Size: 1030 4695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI