Restriction map for
pFN21AB6420
(Circular) (Six-base) MAPSORT of: pFN21AB6420.seq Check: 9881 from: 1 to: 5722
pFN21AB6420 5722 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2062 3065 1937
Size: 125 1003 4594
Fragments arranged by size:
4594 1003 125
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2579 3785 5169 5437 1632
Size: 620 327 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 620 327 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 2931 2963 2114
Size: 32 785 32 4873
Fragments arranged by size:
4873 785 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2071 3465 5037 2071
Size: 1394 1572 2756
Fragments arranged by size:
2756 1572 1394
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2879 3031 3257 3409 5518 1493
Size: 1386 152 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 226 152 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2319 2958 3047 1870
Size: 73 99 277 639 89 4545
Fragments arranged by size:
4545 639 277 99 89 73
AvrII C'CTAG_G
Cuts at: 2990 2990
Size: 5722
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2245 3043 4498 618
Size: 325 217 627 458 798 1455 1842
Fragments arranged by size:
1842 1455 798 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 2320 2959 3041 1391
Size: 875 54 639 82 4072
Fragments arranged by size:
4072 875 639 82 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4751
BbvCI CC'TCA_GC
Cuts at: 2013 2013
Size: 5722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2300 2334 3913 3947 1903
Size: 34 363 34 1579 34 3678
Fragments arranged by size:
3678 1579 363 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4333 136
Size: 107 122 71 631 605 2661 1525
Fragments arranged by size:
2661 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 2895 3139 2895
Size: 244 5478
Bme1580I G_kGCm'C
Cuts at: 1790 3783 4939 1790
Size: 1993 1156 2573
Fragments arranged by size:
2573 1993 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3453 4411 442
Size: 416 53 83 399 24 2036 958 1753
Fragments arranged by size:
2036 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 4382 1239
Size: 1464 1679 2579
Fragments arranged by size:
2579 1679 1464
Bpu10I CC'TnA_GC
Cuts at: 2013 2013
Size: 5722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2739 3847 4712 5010 2171
Size: 568 1108 865 298 2883
Fragments arranged by size:
2883 1108 865 568 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 2609 5716 2609
Size: 3107 2615
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2845 4155 4553 4827 4974 5385 2845
Size: 1310 398 274 147 411 3182
Fragments arranged by size:
3182 1310 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 2501 2531 2931
Size: 30 165 30 711 30 357 30 400
Cuts at: 2931 2961 1178
Size: 30 3939
Fragments arranged by size:
3939 711 400 357 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5722
BseYI C'CCAG_C
Cuts at: 2288 4925 5421 2288
Size: 2637 496 2589
Fragments arranged by size:
2637 2589 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3783 3868 4939 3783
Size: 85 1071 4566
Fragments arranged by size:
4566 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2320 2959 3041 3783 3868 4939
Size: 399 476 54 639 82 742 85 1071
Cuts at: 4939 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 639 476 399 85 82
54
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2353 2532 4217 4391 65
Size: 1183 1105 179 1685 174 1396
Fragments arranged by size:
1685 1396 1183 1105 179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 4366 4553 1700
Size: 110 292 2264 187 2869
Fragments arranged by size:
2869 2264 292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 2052 4526 2052
Size: 2474 3248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2652 3052 3419 3804 3821 5273 5359 5371
Size: 680 400 367 385 17 1452 86 12
Cuts at: 5371 5717 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 680 400 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2033 2430 513
Size: 552 939 29 397 3805
Fragments arranged by size:
3805 939 552 397 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2415 3238 4032 4052 2415
Size: 823 794 20 4085
Fragments arranged by size:
4085 823 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3169 4058 5687
Size: 54 1425 50 275 18 1339 889 1629
Cuts at: 5687 8
Size: 43
Fragments arranged by size:
1629 1425 1339 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 2616 2677 2922 3652 1353
Size: 852 411 61 245 730 3423
Fragments arranged by size:
3423 852 730 411 245 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2579 2703 3785 4382 5169 5437
Size: 393 620 327 124 1082 597 787 268
Cuts at: 5437 1239
Size: 1524
Fragments arranged by size:
1524 1082 787 620 597 393 327 268
124
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 5722
EcoRI G'AATT_C
Cuts at: 2879 3031 3409 2879
Size: 152 378 5192
Fragments arranged by size:
5192 378 152
EcoRV GAT'ATC
Cuts at: 2611 2611
Size: 5722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2688 2688
Size: 5722
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4363 4395 4437 4469 1373
Size: 32 135 32 2791 32 42 32 2626
Fragments arranged by size:
2791 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 3066 3312 669
Size: 988 281 346 782 246 3079
Fragments arranged by size:
3079 988 782 346 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 4836 5020 1305
Size: 1182 2349 184 2007
Fragments arranged by size:
2349 2007 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3900
Fragments arranged by size:
3900 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3680 4039 4198 518
Size: 648 2514 359 159 2042
Fragments arranged by size:
2514 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2035 2314 3138 3815 4963 5208 1606
Size: 423 6 279 824 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 423 279 245 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 2430 513
Size: 552 939 426 3805
Fragments arranged by size:
3805 939 552 426
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 3080 4625 1132
Size: 97 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5722
PfoI T'CCnGG_A
Cuts at: 2108 2118 2108
Size: 10 5712
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2114 2146 2931 2963 3791 3823 5304 5336 2114
Size: 32 785 32 828 32 1481 32 2500
Fragments arranged by size:
2500 1481 828 785 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 2333 3292 2333
Size: 959 4763
PstI C_TGCA'G
Cuts at: 830 3074 830
Size: 2244 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2029 2029
Size: 5722
SacII CC_GC'GG
Cuts at: 2036 2036
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 3070 4205 4886 5077 826
Size: 219 2025 1135 681 191 1471
Fragments arranged by size:
2025 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 2960 3049 1872
Size: 1088 89 4545
Fragments arranged by size:
4545 1088 89
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2319 2754 3826 4727 4989
Size: 197 926 207 169 435 1072 901 262
Cuts at: 4989 820
Size: 1553
Fragments arranged by size:
1553 1072 926 901 435 262 207 197
169
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 2579 3646 5
Size: 47 2527 1067 2081
Fragments arranged by size:
2527 2081 1067 47
StuI AGG'CCT
Cuts at: 1517 2716 1517
Size: 1199 4523
StyI C'CwwG_G
Cuts at: 513 1065 2004 2430 2862 2990 3161 513
Size: 552 939 426 432 128 171 3074
Fragments arranged by size:
3074 939 552 432 426 171 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2999 3749 3766 3919 4104 4549 325
Size: 1255 1419 750 17 153 185 445 1498
Fragments arranged by size:
1498 1419 1255 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3968 5680 96
Size: 274 80 33 51 1443 1991 1712 138
Fragments arranged by size:
1991 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 2299 3058 2299
Size: 759 4963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2428
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 2319 1943
Size: 376 5346
XmaI C'CCGG_G
Cuts at: 1870 2958 3047 1870
Size: 1088 89 4545
Fragments arranged by size:
4545 1088 89
XmnI GAAnn'nnTTC
Cuts at: 2129 2729 3851 2129
Size: 600 1122 4000
Fragments arranged by size:
4000 1122 600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI