Restriction map for
pFN21AB6418
(Circular) (Six-base) MAPSORT of: pFN21AB6418.seq Check: 7523 from: 1 to: 5716
pFN21AB6418 5716 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2325 3059 1937
Size: 388 734 4594
Fragments arranged by size:
4594 734 388
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3779 5163 5431 1632
Size: 2147 1384 268 1917
Fragments arranged by size:
2147 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 2072 2075 1166
Size: 906 3 4807
Fragments arranged by size:
4807 906 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 2185 3459 5031 2185
Size: 1274 1572 2870
Fragments arranged by size:
2870 1572 1274
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2418 2451 3025 3251 3403 5512 1493
Size: 925 33 574 226 152 2109 1697
Fragments arranged by size:
2109 1697 925 574 226 152 33
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 3041 1870
Size: 73 528 570 4545
Fragments arranged by size:
4545 570 528 73
BamHI G'GATC_C
Cuts at: 2937 3046 3413 2937
Size: 109 367 5240
Fragments arranged by size:
5240 367 109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2708 2815 3037 4492 618
Size: 325 217 627 921 107 222 1455 1842
Fragments arranged by size:
1842 1455 921 627 325 222 217 107
BanII G_rGCy'C
Cuts at: 1391 2015 2388 2472 2628 3035 1391
Size: 624 373 84 156 407 4072
Fragments arranged by size:
4072 624 407 373 156 84
BbeI G_GCGC'C
Cuts at: 2712 2712
Size: 5716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 928
Size: 971 741 4004
Fragments arranged by size:
4004 971 741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2323 3608 4818 2323
Size: 1285 1210 3221
Fragments arranged by size:
3221 1285 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2837 4327 136
Size: 107 122 71 631 605 1165 1490 1525
Fragments arranged by size:
1525 1490 1165 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 3777 4933 1790
Size: 1987 1156 2573
Fragments arranged by size:
2573 1987 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2990 4376 1239
Size: 1751 1386 2579
Fragments arranged by size:
2579 1751 1386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 3841 4706 5004 2042
Size: 1799 865 298 2754
Fragments arranged by size:
2754 1799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2709 3523 3905 4540
Size: 53 83 186 936 1176 814 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1176 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2763 2793 1178
Size: 30 165 30 1360 30 4101
Fragments arranged by size:
4101 1360 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2489 1186
Size: 1021 282 4413
Fragments arranged by size:
4413 1021 282
BseYI C'CCAG_C
Cuts at: 2218 2399 4919 5415 2218
Size: 181 2520 496 2519
Fragments arranged by size:
2520 2519 496 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2015 2388 2472 2826 3777 3862 4933 2015
Size: 373 84 354 951 85 1071 2798
Fragments arranged by size:
2798 1071 951 373 354 85 84
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2388 2472 2628 2826 3035 3777
Size: 399 225 373 84 156 198 209 742
Cuts at: 3777 3862 4933 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 225 209 198
156 85 84
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2974 3603 1394
Size: 429 1151 629 3507
Fragments arranged by size:
3507 1151 629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2747 4360 4547 1700
Size: 110 937 1613 187 2869
Fragments arranged by size:
2869 1613 937 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2493 794
Size: 1699 4017
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2704 2782 2854 2077
Size: 627 78 72 4939
Fragments arranged by size:
4939 627 78 72
BstYI r'GATC_y
Cuts at: 1972 2937 3046 3413 3798 3815 5267 5353 5365
Size: 965 109 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 965 385 367 346 109 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 4156
Fragments arranged by size:
4156 1008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2369 487
Size: 683 56 1143 3834
Fragments arranged by size:
3834 1143 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 2967 3232 4026 4046 2491
Size: 476 265 794 20 4161
Fragments arranged by size:
4161 794 476 265 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2154 2209
Size: 54 1425 50 275 18 249 75 55
Cuts at: 2209 2361 2745 3163 4052 5681 8
Size: 152 384 418 889 1629 43
Fragments arranged by size:
1629 1425 889 418 384 275 249 152
75 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2516 3646 1353
Size: 1163 1130 3423
Fragments arranged by size:
3423 1163 1130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2013 2386 2470 2013
Size: 373 84 5259
Fragments arranged by size:
5259 373 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2990 3779 4376 5163 5431 1239
Size: 393 1358 789 597 787 268 1524
Fragments arranged by size:
1524 1358 789 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2418 2451 3025 3403 2418
Size: 33 574 378 4731
Fragments arranged by size:
4731 574 378 33
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2712 4863 1678
Size: 1034 2151 2531
Fragments arranged by size:
2531 2151 1034
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 2864 2896 4357
Size: 32 135 32 1028 32 232 32 1461
Cuts at: 4357 4389 4431 4463 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1461 1028 232 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KasI G'GCGC_C
Cuts at: 2708 2708
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3363
Fragments arranged by size:
3363 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2075 2702 2871 3674 4033 4192
Size: 648 906 3 627 169 803 359 159
Cuts at: 4192 518
Size: 2042
Fragments arranged by size:
2042 906 803 648 627 359 169 159
3
MspA1I CmG'CkG
Cuts at: 1606 2954 3132 3809 4957 5202 1606
Size: 1348 178 677 1148 245 2120
Fragments arranged by size:
2120 1348 1148 677 245 178
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 5716
NarI GG'CG_CC
Cuts at: 2709 2709
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2597 3074 4619 1132
Size: 97 1368 477 1545 2229
Fragments arranged by size:
2229 1545 1368 477 97
PasI CC'CwG_GG
Cuts at: 1348 2639 1348
Size: 1291 4425
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 2530 2573 1108
Size: 1077 345 43 4251
Fragments arranged by size:
4251 1077 345 43
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 2426 3286 2426
Size: 860 4856
PspXI vC'TCGA_Gb
Cuts at: 2471 2471
Size: 5716
PstI C_TGCA'G
Cuts at: 830 2799 3068 830
Size: 1969 269 3478
Fragments arranged by size:
3478 1969 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SacI G_AGCT'C
Cuts at: 2015 2388 2472 2015
Size: 373 84 5259
Fragments arranged by size:
5259 373 84
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 2795 3064 4199 4880 5071 826
Size: 219 1750 269 1135 681 191 1471
Fragments arranged by size:
1750 1471 1135 681 269 219 191
SfoI GGC'GCC
Cuts at: 2710 2710
Size: 5716
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2471 3820 4721 4983 820
Size: 197 926 114 414 1349 901 262 1553
Fragments arranged by size:
1553 1349 926 901 414 262 197 114
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2832 3155 513
Size: 552 1767 323 3074
Fragments arranged by size:
3074 1767 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2430 2788 3743 3760 3913 4098 4543
Size: 1255 850 358 955 17 153 185 445
Cuts at: 4543 325
Size: 1498
Fragments arranged by size:
1498 1255 955 850 445 358 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 3962 5674
Size: 274 80 33 51 1443 135 1850 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1850 1712 1443 274 138 135 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 5676 5708 2763
Size: 32 2881 32 2771
Fragments arranged by size:
2881 2771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 2471 1943
Size: 528 5188
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 2451 2882 3845 2451
Size: 431 963 4322
Fragments arranged by size:
4322 963 431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI